44. C. Suarez et al., Open membranes are the precursors for assembly of large DNA viruses.
Cell. Microbiol. 15(11), 1883–95 (2013)
45. C. Suarez et al., African swine fever virus assembles a single membrane derived from
rupture of the endoplasmic reticulum. Cell. Microbiol. 17(11), 1683–98 (2015)
46. R.L. Felts et al., 3D visualization of HIV transfer at the virological synapse between
dendritic cells and T cells. Proc. Natl. Acad. Sc.i U. S. A. 107(30), 13336–41 (2010)
47. A.A. Linaroudis, Interpretation of electron tomograms of biological specimens by means of
the Scaling Index Method, in Faculty of Chemistry. Technische Universität München (2006)
48. Y. Cheng et al., A primer to single-particle cryo-electron microscopy. Cell 161(3), 438–49
(2015)
49. A. Leschziner, The orthogonal tilt reconstruction method. Methods Enzymol. 482, 237–62
(2010)
50. J.O. Ortiz et al., Mapping 70S ribosomes in intact cells by cryoelectron tomography and
pattern recognition. J. Struct. Biol. 156(2), 334–41 (2006)
51. M. Beck et al., Visual proteomics of the human pathogenLeptospira interrogans. Nat.
Methods 6(11), 817–U55 (2009)
52. F. Forster, B.G. Han, M. Beck, Visual proteomics. Methods Enzymol. 483, 215–43 (2010)
53. M. Beck et al., Exploring the spatial and temporal organization of a cell’s proteome.
J. Struct. Biol. 173(3), 483–96 (2011)
54. J.A. Briggs, Structural biology in situ–the potential of subtomogram averaging. Curr. Opin.
Struct. Biol. 23(2), 261–7 (2013)
55. K. Grunewald et al., Three-dimensional structure of herpes simplex virus from cryo-electron
tomography. Science 302(5649), 1396–8 (2003)
56. N. Grigorieff, S.C. Harrison, Near-atomic resolution reconstructions of icosahedral viruses
from electron cryo-microscopy. Curr. Opin. Struct. Biol. 21(2), 265–73 (2011)
57. Baker, T.S., N.H. Olson, S.D. Fuller, Adding the third dimension to virus life cycles:
three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs.
Microbiol. Mol. Biol. Rev. 63(4), 862–922 (1999) table of contents
58. B.A. Afzelius, A.B. Maunsbach, Biological ultrastructure research; the first 50 years. Tissue
Cell 36(2), 83–94 (2004)
59. K.L. McDonald, R.I. Webb, Freeze substitution in 3 hours or less. J. Microsc. 243(3), 227–
233 (2011)
60. J.J. Wolosewick, K.R. Porter, Microtrabecular lattice of the cytoplasmic ground substance.
Artifact or reality. J. Cell. Biol. 82(1), 114–39 (1979)
61. J. Heuser, Whatever happened to the ‘microtrabecular concept’? Biol. Cell 94(9), 561–96
(2002)
62. M.H. Ellisman, K.R. Porter, Microtrabecular structure of the axoplasmic matrix: visualization of cross-linking structures and their distribution. J. Cell. Biol. 87(2 Pt 1), 464–79 (1980)
63. H. Kondo, On the real structure of the cytoplasmic matrix: learning from the
embedment-free electron microscopy. Arch. Histol. Cytol. 58(4), 397–415 (1995)
64. M. Gruska et al., Electron tomography of vitreous sections from cultured mammalian cells.
J. Struct. Biol. 161(3), 384–392 (2008)
65. T. Wagenknecht, C. Hsieh, M. Marko, Skeletal muscle triad junction ultrastructure by
focused-ion-beam milling of muscle and cryo-electron tomography. Eur. J. Transl. Myol. 25
(1), 4823 (2015)
66. Y. Fukuda, A. Leis, A. Rigort, Preparation of vitrified cells for TEM by Cryo-FIB
Microscopy, in Biological Field Emission Scanning Electron Microscopy, ed. by B. Humbel,
R. Fleck (2016)
67. M. Marko et al., Focused-ion-beam thinning of frozen-hydrated biological specimens for
cryo-electron microscopy. Nat. Methods 4(3), 215–217 (2007)
68. A. Schertel et al., Cryo FIB-SEM: volume imaging of cellular ultrastructure in native frozen
specimens. J. Struct. Biol. 184(2), 355–360 (2013)
69. B.D. Engel, et al., Native architecture of the Chlamydomonas chloroplast revealed by in situ
cryo-electron tomography. Elife 4 (2015)
1 Electron Tomography: A Primer
29
Cell. Microbiol. 15(11), 1883–95 (2013)
45. C. Suarez et al., African swine fever virus assembles a single membrane derived from
rupture of the endoplasmic reticulum. Cell. Microbiol. 17(11), 1683–98 (2015)
46. R.L. Felts et al., 3D visualization of HIV transfer at the virological synapse between
dendritic cells and T cells. Proc. Natl. Acad. Sc.i U. S. A. 107(30), 13336–41 (2010)
47. A.A. Linaroudis, Interpretation of electron tomograms of biological specimens by means of
the Scaling Index Method, in Faculty of Chemistry. Technische Universität München (2006)
48. Y. Cheng et al., A primer to single-particle cryo-electron microscopy. Cell 161(3), 438–49
(2015)
49. A. Leschziner, The orthogonal tilt reconstruction method. Methods Enzymol. 482, 237–62
(2010)
50. J.O. Ortiz et al., Mapping 70S ribosomes in intact cells by cryoelectron tomography and
pattern recognition. J. Struct. Biol. 156(2), 334–41 (2006)
51. M. Beck et al., Visual proteomics of the human pathogenLeptospira interrogans. Nat.
Methods 6(11), 817–U55 (2009)
52. F. Forster, B.G. Han, M. Beck, Visual proteomics. Methods Enzymol. 483, 215–43 (2010)
53. M. Beck et al., Exploring the spatial and temporal organization of a cell’s proteome.
J. Struct. Biol. 173(3), 483–96 (2011)
54. J.A. Briggs, Structural biology in situ–the potential of subtomogram averaging. Curr. Opin.
Struct. Biol. 23(2), 261–7 (2013)
55. K. Grunewald et al., Three-dimensional structure of herpes simplex virus from cryo-electron
tomography. Science 302(5649), 1396–8 (2003)
56. N. Grigorieff, S.C. Harrison, Near-atomic resolution reconstructions of icosahedral viruses
from electron cryo-microscopy. Curr. Opin. Struct. Biol. 21(2), 265–73 (2011)
57. Baker, T.S., N.H. Olson, S.D. Fuller, Adding the third dimension to virus life cycles:
three-dimensional reconstruction of icosahedral viruses from cryo-electron micrographs.
Microbiol. Mol. Biol. Rev. 63(4), 862–922 (1999) table of contents
58. B.A. Afzelius, A.B. Maunsbach, Biological ultrastructure research; the first 50 years. Tissue
Cell 36(2), 83–94 (2004)
59. K.L. McDonald, R.I. Webb, Freeze substitution in 3 hours or less. J. Microsc. 243(3), 227–
233 (2011)
60. J.J. Wolosewick, K.R. Porter, Microtrabecular lattice of the cytoplasmic ground substance.
Artifact or reality. J. Cell. Biol. 82(1), 114–39 (1979)
61. J. Heuser, Whatever happened to the ‘microtrabecular concept’? Biol. Cell 94(9), 561–96
(2002)
62. M.H. Ellisman, K.R. Porter, Microtrabecular structure of the axoplasmic matrix: visualization of cross-linking structures and their distribution. J. Cell. Biol. 87(2 Pt 1), 464–79 (1980)
63. H. Kondo, On the real structure of the cytoplasmic matrix: learning from the
embedment-free electron microscopy. Arch. Histol. Cytol. 58(4), 397–415 (1995)
64. M. Gruska et al., Electron tomography of vitreous sections from cultured mammalian cells.
J. Struct. Biol. 161(3), 384–392 (2008)
65. T. Wagenknecht, C. Hsieh, M. Marko, Skeletal muscle triad junction ultrastructure by
focused-ion-beam milling of muscle and cryo-electron tomography. Eur. J. Transl. Myol. 25
(1), 4823 (2015)
66. Y. Fukuda, A. Leis, A. Rigort, Preparation of vitrified cells for TEM by Cryo-FIB
Microscopy, in Biological Field Emission Scanning Electron Microscopy, ed. by B. Humbel,
R. Fleck (2016)
67. M. Marko et al., Focused-ion-beam thinning of frozen-hydrated biological specimens for
cryo-electron microscopy. Nat. Methods 4(3), 215–217 (2007)
68. A. Schertel et al., Cryo FIB-SEM: volume imaging of cellular ultrastructure in native frozen
specimens. J. Struct. Biol. 184(2), 355–360 (2013)
69. B.D. Engel, et al., Native architecture of the Chlamydomonas chloroplast revealed by in situ
cryo-electron tomography. Elife 4 (2015)
1 Electron Tomography: A Primer
29