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74. F. Greve, S. Frerker, A.G. Bittermann, C. Burkhardt, A. Hierlemann, H. Hall, Molecular
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75. B. Maco, M. Cantoni, A. Holtmaat, A. Kreshuk, F.A. Hamprecht, G.W. Knott,
Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites.
Nat. Protoc. 9, 1354–1366 (2014). doi:10.1038/nprot.2014.101
76. D. Drobne, 3D imaging of cells and tissues by focused ion beam/scanning electron
microscopy (FIB/SEM), Nanoimaging (Humana Press, Totowa, NJ, 2013), pp. 275–292
77. A.J. Bushby, K.M.Y. Png, R.D. Young, C. Pinali, C. Knupp, A.J. Quantock, Imaging
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79. B. Maco, A. Holtmaat, M. Cantoni, A. Kreshuk, C.N. Straehle, F.A. Hamprecht, G.W. Knott,
Correlative in Vivo 2 Photon and focused Ion Beam scanning electron microscopy of cortical
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80. B. Maco, A. Holtmaat, M. Cantoni, A. Kreshuk, C.N. Straehle, F.A. Hamprecht, G.W. Knott,
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neurons. Methods Cell Biol. 124, 339–361 (2013). doi:10.1016/B978-0-12-801075-4.00016-1
5 Volume Scanning Electron Microscopy: Serial Block-Face …
147
mammalian cells with ion-abrasion scanning electron microscopy. J. Struct. Biol. 166, 1–7
(2009). doi:10.1016/j.jsb.2008.11.005
65. C. Kizilyaprak, J. Daraspe, B.M. Humbel, Focused ion beam scanning electron microscopy in
biology. J. Microsc. 254, 109–114 (2014). doi:10.1111/jmi.12127
66. C. Villinger, H. Gregorius, C. Kranz, K. Höhn, C. Münzberg, G. von Wichert, B. Mizaikoff,
G. Wanner, P. Walther, FIB/SEM tomography with TEM-like resolution for 3D imaging of
high-pressure frozen cells. Histochem. Cell Biol. 138, 549–556 (2012). doi:10.1007/s00418012-1020-6
67. D.A.M. de Winter, C.T.W.M. Schneijdenberg, M.N. Lebbink, B. Lich, A.J. Verkleij, M.R.
Drury, B.M. Humbel, Tomography of insulating biological and geological materials using
focused ion beam (FIB) sectioning and low-kV BSE imaging. J. Microsc. 233, 372–383
(2009). doi:10.1111/j.1365-2818.2009.03139.x
68. A. Rigort, F.J.B. Bäuerlein, E. Villa, M. Eibauer, T. Laugks, W. Baumeister, J.M. Plitzko,
Focused ion beam micromachining of eukaryotic cells for cryoelectron tomography. Proc.
Natl. Acad. Sci. U.S.A. 109, 4449–4454 (2012). doi:10.1073/pnas.1201333109
69. L. Chatel-Chaix, M. Cortese, I. Romero-Brey, S. Bender, C.J. Neufeldt, W. Fischl,
P. Scaturro, N. Schieber, Y. Schwab, B. Fischer, A. Ruggieri, Dengue virus perturbs
mitochondrial morphodynamics to dampen innate immune responses. Cell Host Microbe 20
(3), 1–16 (2016). doi:10.1016/j.chom.2016.07.008
70. G.E. Murphy, K. Narayan, B.C. Lowekamp, L.M. Hartnell, J.A.W. Heymann, J. Fu, S.
Subramaniam, Correlative 3D imaging of whole mammalian cells with light and electron
microscopy. J. Struct. Biol. 176, 268–278 (2011). doi:10.1016/j.jsb.2011.08.013
71. J. Harapin, M. Börmel, K.T. Sapra, D. Brunner, A. Kaech, O. Medalia, Structural analysis of
multicellular organisms with cryo-electron tomography. Nat. Publishing Group 12, 634–636
(2015). doi:10.1038/nmeth.3401
72. L.H.P. Hekking, M.N. Lebbink, D.A.M. de Winter, C.T.W.M. Schneijdenberg, C.M. Brand,
B.M. Humbel, A.J. Verkleij, J.A. Post, Focused ion beam-scanning electron microscope:
exploring large volumes of atherosclerotic tissue. J. Microsc. 235, 336–347 (2009). doi:10.
1111/j.1365-2818.2009.03274.x
73. S. Hirashima, K. Ohta, T. Kanazawa, S. Okayama, A. Togo, N. Uchimura, J. Kusukawa, K.-I.
Nakamura, Three-dimensional ultrastructural analysis of cells in the periodontal ligament
using focused ion beam/scanning electron microscope tomography. Sci Rep 6, 39435 (2016).
doi:10.1038/srep39435
74. F. Greve, S. Frerker, A.G. Bittermann, C. Burkhardt, A. Hierlemann, H. Hall, Molecular
design and characterization of the neuron-microelectrode array interface. Biomaterials 28,
5246–5258 (2007)
75. B. Maco, M. Cantoni, A. Holtmaat, A. Kreshuk, F.A. Hamprecht, G.W. Knott,
Semiautomated correlative 3D electron microscopy of in vivo-imaged axons and dendrites.
Nat. Protoc. 9, 1354–1366 (2014). doi:10.1038/nprot.2014.101
76. D. Drobne, 3D imaging of cells and tissues by focused ion beam/scanning electron
microscopy (FIB/SEM), Nanoimaging (Humana Press, Totowa, NJ, 2013), pp. 275–292
77. A.J. Bushby, K.M.Y. Png, R.D. Young, C. Pinali, C. Knupp, A.J. Quantock, Imaging
three-dimensional tissue architectures by focused ion beam scanning electron microscopy.
Nat. Protoc. 6, 845–858 (2011). doi:10.1038/nprot.2011.332
78. L.A. Giannuzzi, F.A. Stevie, Introduction to Focused Ion Beams (Springer, Boston, 2005)
79. B. Maco, A. Holtmaat, M. Cantoni, A. Kreshuk, C.N. Straehle, F.A. Hamprecht, G.W. Knott,
Correlative in Vivo 2 Photon and focused Ion Beam scanning electron microscopy of cortical
Neurons. PLoS ONE 8, e57405 (2013). doi:10.1371/journal.pone.0057405.g003
80. B. Maco, A. Holtmaat, M. Cantoni, A. Kreshuk, C.N. Straehle, F.A. Hamprecht, G.W. Knott,
Correlative in vivo 2 photon and focused ion beam scanning electron microscopy of cortical
neurons. Methods Cell Biol. 124, 339–361 (2013). doi:10.1016/B978-0-12-801075-4.00016-1
5 Volume Scanning Electron Microscopy: Serial Block-Face …
147