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148
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Site-specific 3D imaging of cells and tissues with a dual beam microscope. J. Struct. Biol.
155, 63–73 (2006). doi:10.1016/j.jsb.2006.03.006
82. E. Brama, C.J. Peddie, G. Wilkes, Y. Gu, L.M. Collinson, M.L. Jones, ultraLM and miniLM:
Locator tools for smart tracking of fluorescent cells in correlative light and electron
microscopy. Wellcome Open Res. 1, 19–26 (2016). doi:10.12688/wellcomeopenres.10299.1
83. A. Schertel, N. Snaidero, H.-M. Han, T. Ruhwedel, M. Laue, M. Grabenbauer, W. Möbius,
Cryo FIB-SEM: Volume imaging of cellular ultrastructure in native frozen specimens.
J. Struct. Biol. 184, 355–360 (2013). doi:10.1016/j.jsb.2013.09.024
84. J. Arnold, J. Mahamid, V. Lucic, A. de Marco, J.J. Fernandez, T. Laugks, T. Mayer, A.A.
Hyman, W. Baumeister, J.M. Plitzko, Site-specific cryo-focused ion beam sample preparation
guided by 3D correlative microscopy. Biophysj 110(4), 1–10 (2016). doi:10.1016/j.bpj.2015.
10.053
85. J. Mahamid, S. Pfeffer, M. Schaffer, E. Villa, R. Danev, L.K. Cuellar, F. Förster, A.A.
Hyman, J.M. Plitzko, W. Baumeister, Visualizing the molecular sociology at the HeLa cell
nuclear periphery. Science 351, 969–972 (2016). doi:10.1126/science.aad8857
86. S. Borrett, L. Hughes, Reporting methods for processing and analysis of data from serial
block face scanning electron microscopy. J. Microsc. 263, 3–9 (2016). doi:10.1111/jmi.12377
87. A. Cardona, S. Saalfeld, J. Schindelin, I. Arganda-Carreras, S. Preibisch, M. Longair,
P. Tomancak, V. Hartenstein, R.J. Douglas, TrakEM2 software for neural circuit reconstruction. PLoS ONE 7, e38011 (2012). doi:10.1371/journal.pone.0038011
88. T. Pietzsch, S. Saalfeld, S. Preibisch, P. Tomancak, BigDataViewer: visualization and
processing for large image data sets. Nat. Publishing Group 12, 481–483 (2015). doi:10.1038/
nmeth.3392
89. P. Thévenaz, U.E. Ruttimann, M. Unser, A pyramid approach to subpixel registration based
on intensity. IEEE Trans. Image Process. 7, 27–41 (1998). doi:10.1109/83.650848
90. A. Kreshuk, C.N. Straehle, C. Sommer, U. Koethe, M. Cantoni, G. Knott, F.A. Hamprecht,
Automated detection and segmentation of synaptic contacts in nearly isotropic serial electron
microscopy images. PLoS ONE 6, e24899 (2011). doi:10.1371/journal.pone.0024899
91. A. Kreshuk, R. Walecki, U. Koethe, M. Gierthmuehlen, D. Plachta, C. Genoud, K.
Haastert-talini, F.A. Hamprecht, Automated tracing of myelinated axons and detection of the
nodes of Ranvier in serial images of peripheral nerves. J. Microsc. 259, 143–154 (2015).
doi:10.1111/jmi.12266
92. I. Belevich, M. Joensuu, D. Kumar, H. Vihinen, E. Jokitalo, Microscopy image browser: a
platform for segmentation and analysis of multidimensional datasets. PLoS Biol. 14,
e1002340 (2016). doi:10.1371/journal.pbio.1002340
93. A.L. Keller, D. Zeidler, T. Kemen, High-throughput data acquisition with a multi-beam SEM.
Scan. Microscopies 9236 (2014)
94. T. Kemen, M. Malloy, B. Thiel, S. Mikula, W. Denk, G. Dellemann, D. Zeidler, Further
advancing the throughput of a multi-beam SEM. Metrology, Inspection, and Process Control
for Microlithography XXIX. (2015). doi:10.1117/12.2188560
95. P. Kruit, Y. Ren, Multi-Beam Scanning Electron Microscope Design. Microsc. Microanal. 22,
574–575 (2016). doi:10.1017/S143192761600372X
96. A.L. Eberle, S. Mikula, R. Schlek, J. Lichtman, M.L. Knothe Tate, D. Zeidler,
High-resolution, high-throughput imaging with a multibeam scanning electron microscope.
J. Microsc. 259, 114–120 (2015). doi:10.1111/jmi.12224
97. D. Drouin, A.R. Couture, D. Joly, X. Tastet, V. Aimez, R. Gauvin, CASINO V2.42: a fast and
easy-to-use modeling tool for scanning electron microscopy and microanalysis users.
Scanning 29, 92–101 (2007). doi:10.1002/sca.20000
98. L. Hughes, K. Towers, T. Starborg, K. Gull, S. Vaughan, A cell-body groove housing the new
flagellum tip suggests an adaption of cellular morphogenesis for parasitism in the bloodstream
form of Trypanosoma brucei. J. Cell Sci. 126, 5748–5757 (2013). doi:10.1242/jcs.139139
148
R. I. Webb and N. L. Schieber