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146
R. I. Webb and N. L. Schieber
digital imaging technology reduce turnaround times for diagnostic electron microscopy.
Ultrastruct. Pathol. 27, 187–196 (2003). doi:10.1080/01913120309937
45. N.L. Schieber, S.J. Nixon, R.I. Webb, V.M.J. Oorschot, R.G. Parton, Modern approaches for
ultrastructural analysis of the zebrafish embryo. Methods Cell Biol. 96, 425–442 (2010).
doi:10.1016/S0091-679X(10)96018-4
46. A.A. Wanner, M.A. Kirschmann, C. Genoud, Challenges of microtome-based serial
block-face scanning electron microscopy in neuroscience. J. Microsc. 259, 137–142
(2015). doi:10.1111/jmi.12244
47. H.B. Nguyen, T.Q. Thai, S. Saitoh, B. Wu, Y. Saitoh, S. Shimo, H. Fujitani, H. Otobe, N.
Ohno, Conductive resins improve charging and resolution of acquired images in electron
microscopic volume imaging. Sci. Rep. 6, 1–10 (2016). doi:10.1038/srep23721
48. D. Studer, H. Gnaegi, Minimal compression of ultrathin sections with use of an oscillating
diamond knife. J. Microsc. 197, 94–100 (2000)
49. W.C. De Bruijn, Glycogen, its chemistry and morphologic appearance in the electron
microscope: I. A modified OsO4 fixative which selectively contrasts glycogen. J. Ultrastruct.
Res. 42(1–2), 1–22 (1973)
50. A.M. Seligman, H.L. Wasserkrug, J.S. Hanker, A new staining method (OTO) for enhancing
contrast of lipid-containing membranes and droplets in osmium tetroxide-fixed tissue with
osmiophilic thiocarbohydrazide (TCH). J. Cell Biol. 30(2), 1–9 (1966)
51. J. Walton, Lead asparate, an en bloc contrast stain particularly useful for ultrastructural
enzymology. J. Histochem. Cytochem. 27, 1337–1342 (1979). doi:10.1177/27.10.512319
52. Y. Hua, P. Laserstein, M. Helmstaedter, Large-volume en-bloc staining for electron
microscopy-based connectomics. Nat. Commun. 6, 1–7 (2015). doi:10.1038/ncomms8923
53. S. Mikula, J. Binding, W. Denk, Staining and embedding the whole mouse brain for electron
microscopy. Nat. Meth. 9, 1198–1201 (2012). doi:10.1038/nmeth.2213
54. R. Webb, R. Webb, Quick freeze substitution processing of biological samples for serial
block-face scanning electron microscopy. Microsc. Microanal. 21, 1115–1116 (2015). doi:10.
1017/S1431927615006364
55. K.L. McDonald, R.I. Webb, Freeze substitution in 3 hours or less. J. Microsc. 243, 227–233
(2011). doi:10.1111/j.1365-2818.2011.03526.x
56. H. Kushida, Block staining with lead acetate. J. Electron Microsc. 15, 90–92 (1966)
57. D. Scheuring, C. Löfke, F. Krüger, M. Kittelmann, A. Eisa, L. Hughes, R.S. Smith, C. Hawes,
K. Schumacher, J. Kleine-Vehn, Actin-dependent vacuolar occupancy of the cell determines
auxin-induced growth repression. Proc. Natl. Acad. Sci. U.S.A. 113, 452–457 (2016). doi:10.
1073/pnas.1517445113
58. S. Mikula, W. Denk, High-resolution whole-brain staining for electron microscopic circuit
reconstruction. Nat Meth 12, 541–546 (2015). doi:10.1038/nmeth.3361
59. K.M. Furuta, S.R. Yadav, S. Lehesranta, I. Belevich, S. Miyashima, J. Heo, A. Vatén, O.
Lindgren, B. de Rybel, G. van Isterdael, P. Somervuo, R. Lichtenberger, R. Rocha, S.
Thitamadee, S. Tähtiharju, P. Auvinen, T. Beeckman, E. Jokitalo, Y. Helariutta, Arabidopsis
NAC45/86 direct sieve element morphogenesis culminating in enucleation. Science 345, 933–
937 (2014). doi:10.1126/science.1255097
60. T. Starborg, N.S. Kalson, Y. Lu, A. Mironov, T.F. Cootes, D.F. Holmes, K.E. Kadler, Using
transmission electron microscopy and 3View to determine collagen fibril size and
three-dimensional organization. Nat. Protoc. 8, 1433–1448 (2013). doi:10.1038/nprot.2013.086
61. T. Anttonen, I. Belevich, A. Kirjavainen, M. Laos, C. Brakebusch, E. Jokitalo, U. Pirvola,
How to bury the dead: elimination of apoptotic hair cells from the hearing organ of the mouse.
JARO 15, 975–992 (2014). doi:10.1007/s10162-014-0480-x
62. M. Helmstaedter, Cellular-resolution connectomics: challenges of dense neural circuit
reconstruction. Nat Meth 10, 501–507 (2013). doi:10.1038/nmeth.2476
63. K.L. Briggman, M. Helmstaedter, W. Denk, Wiring specificity in the direction-selectivity
circuit of the retina. Nature 471, 183–188 (2011). doi:10.1038/nature09818
146
R. I. Webb and N. L. Schieber