gold sulfur chemistry, 52
IDEs, 28, 29
measurement cell, 33
MWCNT, 21
nanogap, 31
pencil graphite, 27
Electromobility shift assay (EMSA), 3, 5, 8,
9, 12
Environmental application
drug residues
anthracycline, 63
antibiotics, 63
hormones, 63–64
toxins, 64
impedimetric biosensors, 67
F
Faradaic impedimetric aptasensors, 22–28
Filter-binding (FB) assay, 3, 5, 8, 9, 11
Flow cytometry, 3, 5, 7–9, 12, 148, 171
Fluorescence anisotropy (FA), 3, 7
Fluorescence polarization (FP), 3, 5, 7, 8, 9, 12
Food industry
impedimetric aptasensors
microorganisms, 73–77
mycotoxins, 69–73
manufacturing and handling, 67
G
Gold nanoparticles (AuNPs), 164–168,
172–175, 183
Group-specific proteins, 122–124
H
Health
care (see Health care)
individuals, 55
workers, 201
Health care
cancer detection
cells, 52–53
oncolytic virus, 57, 59
soluble tumor marker, 53–57
diagnosis of diseases, 46–51
EIS, 61
impedimetric aptasensors, 45
label-free application, 60
use cases, 59–61
Hormones, 46, 49, 62–64, 206
Hybrid molecular imprinting system, 28
I
Impedance
advantages, 18
combination, 19
electrochemical aptasensors, 19
measurement techniques, 20–22
Infection, 46, 47, 50, 57, 73, 201, 205
Isothermal titration calorimetry (ICT), 3, 5, 7, 9,
10, 12
K
Kinetics
development phase, 3
ITC, 7
parameters, 4
L
Label-free
aptamer-modified nanoparticles, 170
cancer cells, 52
detection strategies, 19
impedimetric systems, 22
MST and ITC, 9
Lateral flow assays (LFAs), 165–166
Ligand
aptamer-protein interaction
electrostatic interactions, 103
hydrogen bonding, 103–104
hydrophobic associations, 104–105
molecular interaction, 105, 106
thermodynamics bases, 105, 106
oligonucleotide libraries, 99
progression, 98
RNA vs. DNA, 111–112
SELEX, 106–108
single-chain oligonucleotide, 99–102
Lipopolysaccharide (LPS), 59, 73
Liposomes, 162, 173, 175–177
M
Micelles, 175–177
Microorganisms, 62, 65, 73–77
MicroScale thermophoresis (MST), 3, 5–12
Molecular imaging
alternative/complementary diagnostic tool,
150
antibodies, 143
CT and MR, 153
image-based detection, 150
modalities, 150
Index
213
IDEs, 28, 29
measurement cell, 33
MWCNT, 21
nanogap, 31
pencil graphite, 27
Electromobility shift assay (EMSA), 3, 5, 8,
9, 12
Environmental application
drug residues
anthracycline, 63
antibiotics, 63
hormones, 63–64
toxins, 64
impedimetric biosensors, 67
F
Faradaic impedimetric aptasensors, 22–28
Filter-binding (FB) assay, 3, 5, 8, 9, 11
Flow cytometry, 3, 5, 7–9, 12, 148, 171
Fluorescence anisotropy (FA), 3, 7
Fluorescence polarization (FP), 3, 5, 7, 8, 9, 12
Food industry
impedimetric aptasensors
microorganisms, 73–77
mycotoxins, 69–73
manufacturing and handling, 67
G
Gold nanoparticles (AuNPs), 164–168,
172–175, 183
Group-specific proteins, 122–124
H
Health
care (see Health care)
individuals, 55
workers, 201
Health care
cancer detection
cells, 52–53
oncolytic virus, 57, 59
soluble tumor marker, 53–57
diagnosis of diseases, 46–51
EIS, 61
impedimetric aptasensors, 45
label-free application, 60
use cases, 59–61
Hormones, 46, 49, 62–64, 206
Hybrid molecular imprinting system, 28
I
Impedance
advantages, 18
combination, 19
electrochemical aptasensors, 19
measurement techniques, 20–22
Infection, 46, 47, 50, 57, 73, 201, 205
Isothermal titration calorimetry (ICT), 3, 5, 7, 9,
10, 12
K
Kinetics
development phase, 3
ITC, 7
parameters, 4
L
Label-free
aptamer-modified nanoparticles, 170
cancer cells, 52
detection strategies, 19
impedimetric systems, 22
MST and ITC, 9
Lateral flow assays (LFAs), 165–166
Ligand
aptamer-protein interaction
electrostatic interactions, 103
hydrogen bonding, 103–104
hydrophobic associations, 104–105
molecular interaction, 105, 106
thermodynamics bases, 105, 106
oligonucleotide libraries, 99
progression, 98
RNA vs. DNA, 111–112
SELEX, 106–108
single-chain oligonucleotide, 99–102
Lipopolysaccharide (LPS), 59, 73
Liposomes, 162, 173, 175–177
M
Micelles, 175–177
Microorganisms, 62, 65, 73–77
MicroScale thermophoresis (MST), 3, 5–12
Molecular imaging
alternative/complementary diagnostic tool,
150
antibodies, 143
CT and MR, 153
image-based detection, 150
modalities, 150
Index
213