Molecular imaging (cont.)
nucleic acid selection methodologies, 142
optical imaging, 151–152
PET and SPECT, 154–156
targeting components, 150
ultrasound, 154
in vivo characterization, 150
Monitoring
clinical examination, 46
diagnosis, 203
PDGF-BB, 30
refractive index, 4
target-intrinsic tryptophan fluorescence, 9
Mycotoxins, 68–73
N
Nanocomposites
fabrication, 27
glutaraldehyde, 27
gold nanoparticles, 74
graphene oxide, 74
impedimetric biosensors, 28
porous carbon structures, 72
Nanoladders, 24, 25
Nanoparticles
aptamer-coupled, 162
aptamer-modified (see Aptamer-modified
nanoparticles)
coupling, 163
multivalent binding, 163–164
types, 162
Nanotechnology, 19, 80, 162
Non-faradaic impedimetric aptasensors, 28–32
P
Pathogen, 65, 67, 73, 203, 207
Photodynamic therapy
AuNPs, 175, 182
SWNT, 182–184
Photothermal therapy (PTT)
AuNPs, 182
SWNT, 182–184
Point-of-care (POC), 11, 32, 45, 51, 77, 80,
166, 199, 203–205
Polymer nanoparticles, 177–178
Positron emission tomography (PET), 154–156
Probe design, 144–146
Prostate-specific antigen (PSA), 27, 28, 206
Protein purification
acidic pH, 119
affinity chromatography, 112
aptamers, 98
DNA aptamers, 122, 124
homogeneity, 123
oligonucleotide affinity ligand
molecules, 95
R
Rapid detection, 45, 65, 68
Redox mediator, 22–25, 28, 71, 72
S
Serum
bioliquids, 13
biosensors, 47
diluted, 25
spiked, 50
undiluted, 30
VEGF detection, 55
Single photon emission computed tomography
(SPECT), 154–156
Single-walled carbon nanotubes (SWNTs), 163,
182–185
Surface plasmon resonance (SPR), 3, 4, 47,
146, 166
SwitchSense (SWS), 3, 5, 6, 8, 9, 11, 12
Systematic evolution of ligands by exponential
enrichment (SELEX) procedure
affinity chromatography, 107
aptameric ligand selection, 106–108
DNA and RNA, 145
modified nucleotide, 112
process, 79
T
Target product profiles (TPPs)
antibodies, 197
aptamer-specific, 206–207
bacterial infection, 205
benefits of designing, 198
features, 199–202
HIV and adenovirus, 196
POC, 203–205
TB diagnostics, 202–203
Therapy
cancer, 25, 63
extracorporeal, 125
hormone, 64
human, 27
photodynamic, 175, 181–184
214
Index
nucleic acid selection methodologies, 142
optical imaging, 151–152
PET and SPECT, 154–156
targeting components, 150
ultrasound, 154
in vivo characterization, 150
Monitoring
clinical examination, 46
diagnosis, 203
PDGF-BB, 30
refractive index, 4
target-intrinsic tryptophan fluorescence, 9
Mycotoxins, 68–73
N
Nanocomposites
fabrication, 27
glutaraldehyde, 27
gold nanoparticles, 74
graphene oxide, 74
impedimetric biosensors, 28
porous carbon structures, 72
Nanoladders, 24, 25
Nanoparticles
aptamer-coupled, 162
aptamer-modified (see Aptamer-modified
nanoparticles)
coupling, 163
multivalent binding, 163–164
types, 162
Nanotechnology, 19, 80, 162
Non-faradaic impedimetric aptasensors, 28–32
P
Pathogen, 65, 67, 73, 203, 207
Photodynamic therapy
AuNPs, 175, 182
SWNT, 182–184
Photothermal therapy (PTT)
AuNPs, 182
SWNT, 182–184
Point-of-care (POC), 11, 32, 45, 51, 77, 80,
166, 199, 203–205
Polymer nanoparticles, 177–178
Positron emission tomography (PET), 154–156
Probe design, 144–146
Prostate-specific antigen (PSA), 27, 28, 206
Protein purification
acidic pH, 119
affinity chromatography, 112
aptamers, 98
DNA aptamers, 122, 124
homogeneity, 123
oligonucleotide affinity ligand
molecules, 95
R
Rapid detection, 45, 65, 68
Redox mediator, 22–25, 28, 71, 72
S
Serum
bioliquids, 13
biosensors, 47
diluted, 25
spiked, 50
undiluted, 30
VEGF detection, 55
Single photon emission computed tomography
(SPECT), 154–156
Single-walled carbon nanotubes (SWNTs), 163,
182–185
Surface plasmon resonance (SPR), 3, 4, 47,
146, 166
SwitchSense (SWS), 3, 5, 6, 8, 9, 11, 12
Systematic evolution of ligands by exponential
enrichment (SELEX) procedure
affinity chromatography, 107
aptameric ligand selection, 106–108
DNA and RNA, 145
modified nucleotide, 112
process, 79
T
Target product profiles (TPPs)
antibodies, 197
aptamer-specific, 206–207
bacterial infection, 205
benefits of designing, 198
features, 199–202
HIV and adenovirus, 196
POC, 203–205
TB diagnostics, 202–203
Therapy
cancer, 25, 63
extracorporeal, 125
hormone, 64
human, 27
photodynamic, 175, 181–184
214
Index