B
Bacteria
diagnostic detection, 205
Escherichia coli, 65–66
gram-positive, 47
human cells, 10
microorganisms, 73–77
and non-bacterial infections, 205
toxins, 64, 65
Binding parameters
aptamer development, 2–3
aptamer-target interaction, 3
and characteristics, 11
Biolayer interferometry (BLI), 3, 4, 6, 8–13
Biomarker
bacterial and non-bacterial infections, 205
hormones/infection-related components, 46
lysozymes, 47
target tissue, 162
VEGF, 55, 109
Biomedicine, 142
Biophysical characterization
application range, 10–11
BLI, 4, 6
EMSA, 8
filter-binding assay/filter retention assay, 8
flow cytometry, 7
FP/FA, 7
information content, 5
ITC, 7
key advantages, 5
MST, 6–7
readout, 5
sample material requirements, 8–9
SPR, 4
strategy, 11
SwS, 6
Biosensor
aptamer-based (see Aptamer)
CRP determination, 47
environmental application, 67
fluorescence, 169–170
impedimetric aptamer-based
(see Impedance)
intensive investigations, 109
SERS-based, 147
Blood proteins, 117–122
C
Cancer
biomarker, 206
detection
cells, 52–54
oncolytic virus, 57–59
soluble tumor marker, 53, 55–57
multidrug-resistant, 181
theragnostic agents, 164
therapy, 25, 63
Capacitive sensing, 28–31
Contaminants, 59, 63–70, 73, 75, 125
D
Dendrimers, 50, 69, 72, 153, 179, 181
Diagnostics
affinity chromatography, 109
aptamer (see Aptamer)
cancer (see Cancer)
medical, 45
published detection, 36
state-of-the-art, 52 (see also Molecular
imaging)
Disease
diagnosis, 46–51
foodborne, 67, 73, 75
Nampt plasma levels, 29
DNA origami, 25, 26, 36
DNA-related proteins, 122
Drug
anthracycline, 63
antibiotics, 63
aptamer-modified nanoparticles
(see Aptamer-modified
nanoparticles)
delivery, 25
hormones, 63–64
industry, 59
TDM, 60
Drug delivery
AuNPs, 172–175
dendrimers, 179, 181
liposomes, 175–177
mesoporous silica, 178–180
micelles, 175–177
polymer nanoparticles, 177–178
E
Electrode
biosensors, 18
CNT-aptamer, 48
detection range, 72
DNA complement, 24
electron transfer, 24
glassy carbon, 27
212
Index
Bacteria
diagnostic detection, 205
Escherichia coli, 65–66
gram-positive, 47
human cells, 10
microorganisms, 73–77
and non-bacterial infections, 205
toxins, 64, 65
Binding parameters
aptamer development, 2–3
aptamer-target interaction, 3
and characteristics, 11
Biolayer interferometry (BLI), 3, 4, 6, 8–13
Biomarker
bacterial and non-bacterial infections, 205
hormones/infection-related components, 46
lysozymes, 47
target tissue, 162
VEGF, 55, 109
Biomedicine, 142
Biophysical characterization
application range, 10–11
BLI, 4, 6
EMSA, 8
filter-binding assay/filter retention assay, 8
flow cytometry, 7
FP/FA, 7
information content, 5
ITC, 7
key advantages, 5
MST, 6–7
readout, 5
sample material requirements, 8–9
SPR, 4
strategy, 11
SwS, 6
Biosensor
aptamer-based (see Aptamer)
CRP determination, 47
environmental application, 67
fluorescence, 169–170
impedimetric aptamer-based
(see Impedance)
intensive investigations, 109
SERS-based, 147
Blood proteins, 117–122
C
Cancer
biomarker, 206
detection
cells, 52–54
oncolytic virus, 57–59
soluble tumor marker, 53, 55–57
multidrug-resistant, 181
theragnostic agents, 164
therapy, 25, 63
Capacitive sensing, 28–31
Contaminants, 59, 63–70, 73, 75, 125
D
Dendrimers, 50, 69, 72, 153, 179, 181
Diagnostics
affinity chromatography, 109
aptamer (see Aptamer)
cancer (see Cancer)
medical, 45
published detection, 36
state-of-the-art, 52 (see also Molecular
imaging)
Disease
diagnosis, 46–51
foodborne, 67, 73, 75
Nampt plasma levels, 29
DNA origami, 25, 26, 36
DNA-related proteins, 122
Drug
anthracycline, 63
antibiotics, 63
aptamer-modified nanoparticles
(see Aptamer-modified
nanoparticles)
delivery, 25
hormones, 63–64
industry, 59
TDM, 60
Drug delivery
AuNPs, 172–175
dendrimers, 179, 181
liposomes, 175–177
mesoporous silica, 178–180
micelles, 175–177
polymer nanoparticles, 177–178
E
Electrode
biosensors, 18
CNT-aptamer, 48
detection range, 72
DNA complement, 24
electron transfer, 24
glassy carbon, 27
212
Index