7. What is the main enzyme component of Sanger sequencing?
8. Which of the following best describes three cyclic steps in PCR, in the correct order?
A. Denaturing DNA to make it single stranded, primer annealing, synthesis of a new
DNA strand.
B. Primer annealing, denaturing DNA to make it single stranded, synthesis of a new
DNA strand.
C. Synthesis of a new DNA strand, primer annealing, denaturing DNA to make it
single stranded.
9. Which of the following “omes” relates to the DNA sequence of expressable genes?
A. Genome.
B. Exome.
C. Proteome.
D. Metabolome.
10. Targeted sequencing:
A. Allows to focus on specific areas of interest and thus enables sequencing at
much higher coverage levels.
B. Is a sequencing method used within molecular diagnostics.
C. Is equivalent to sequencing tumor panels.
D. This method refers to sequence a novel genome.
11. Library preparation involves generating a collection of DNA fragments for
sequencing. NGS libraries are typically prepared by fragmenting a DNA or RNA
sample and ligating specialized adapters to both fragments ends. Bring the following working steps in terms of library preparation in the right order:
A. Perform End Repair and size selection via AMPure XP Beads.
B. Quantification and profile samples.
C. Purify Ligation Products.
D. Validate Library.
E. Adenylate 3’-Ends.
F. Normalize and pool libraries.
G. Enrich DNA Fragments and size selection via AMPure XP Beads.
H. Adapter Ligation and size selection via AMPure XP Beads.
12. Indicate whether each of the following descriptions better applies to Illumina
®
sequencing (I), Ion Torrent™ sequencing (T), or both sequencing technologies (B).
– It uses fluorescently labeled nucleotides.
– It uses PCR-generated copies of DNA.
4 NGS Technologies
57
8. Which of the following best describes three cyclic steps in PCR, in the correct order?
A. Denaturing DNA to make it single stranded, primer annealing, synthesis of a new
DNA strand.
B. Primer annealing, denaturing DNA to make it single stranded, synthesis of a new
DNA strand.
C. Synthesis of a new DNA strand, primer annealing, denaturing DNA to make it
single stranded.
9. Which of the following “omes” relates to the DNA sequence of expressable genes?
A. Genome.
B. Exome.
C. Proteome.
D. Metabolome.
10. Targeted sequencing:
A. Allows to focus on specific areas of interest and thus enables sequencing at
much higher coverage levels.
B. Is a sequencing method used within molecular diagnostics.
C. Is equivalent to sequencing tumor panels.
D. This method refers to sequence a novel genome.
11. Library preparation involves generating a collection of DNA fragments for
sequencing. NGS libraries are typically prepared by fragmenting a DNA or RNA
sample and ligating specialized adapters to both fragments ends. Bring the following working steps in terms of library preparation in the right order:
A. Perform End Repair and size selection via AMPure XP Beads.
B. Quantification and profile samples.
C. Purify Ligation Products.
D. Validate Library.
E. Adenylate 3’-Ends.
F. Normalize and pool libraries.
G. Enrich DNA Fragments and size selection via AMPure XP Beads.
H. Adapter Ligation and size selection via AMPure XP Beads.
12. Indicate whether each of the following descriptions better applies to Illumina
®
sequencing (I), Ion Torrent™ sequencing (T), or both sequencing technologies (B).
– It uses fluorescently labeled nucleotides.
– It uses PCR-generated copies of DNA.
4 NGS Technologies
57