Review Questions
1. Which of the following statements regarding the quantity of template for a sequencing reaction is correct?
A. Excess template reduces the length of a read.
B. Too little template will result in very little readable sequences.
C. Excess template reduces the quality of a read.
D. All of the above.
2. What will heterozygous single nucleotide substitution look like on your
chromatogram?
A. Two peaks of equal height at the same position.
B. One peak twice the height of those around it.
C. Two peaks in the same position, one twice the height of the other.
D. Three peaks of equal height at the same position.
3. Which of the following is important for preparing templates for Next Generation
Sequencing?
A. Isolating DNA from tissue.
B. Breaking DNA up into smaller fragments.
C. Checking the quality and quantity of the fragment library.
D. All of the above.
4. Which of the below sequencing techniques require DNA amplification during the
library preparation step (is considered a 2nd generation sequencing technique)?
A. PacBio AND Oxford Nanopore.
B. Illumina AND Ion Torrent.
C. Illumina AND Oxford Nanopore.
D. PacBio AND Ion Torrent.
5. Which of the below sequencing techniques use(s) fluorescently labeled nucleotides
for identifying the nucleotide sequence of the template DNA strand?
A. Illumina AND PacBio.
B. Illumina AND Oxford Nanopore.
C. PacBio AND Ion Torrent.
D. Only Illumina.
E. All sequencing methods use fluorescently labeled nucleotides for identifying the
nucleotide sequence of the template DNA strand.
6. The below figures illustrate five cycles of Illumina sequencing. The colored spots
represent the clusters on the flow cell. What is the sequence of the DNA template
(cluster) in the top, left corner according to the figure?
A: Yellow
C: Red
G: Blue
T: Green
56
M. Eisele and M. Kappelmann-Fenzl
1. Which of the following statements regarding the quantity of template for a sequencing reaction is correct?
A. Excess template reduces the length of a read.
B. Too little template will result in very little readable sequences.
C. Excess template reduces the quality of a read.
D. All of the above.
2. What will heterozygous single nucleotide substitution look like on your
chromatogram?
A. Two peaks of equal height at the same position.
B. One peak twice the height of those around it.
C. Two peaks in the same position, one twice the height of the other.
D. Three peaks of equal height at the same position.
3. Which of the following is important for preparing templates for Next Generation
Sequencing?
A. Isolating DNA from tissue.
B. Breaking DNA up into smaller fragments.
C. Checking the quality and quantity of the fragment library.
D. All of the above.
4. Which of the below sequencing techniques require DNA amplification during the
library preparation step (is considered a 2nd generation sequencing technique)?
A. PacBio AND Oxford Nanopore.
B. Illumina AND Ion Torrent.
C. Illumina AND Oxford Nanopore.
D. PacBio AND Ion Torrent.
5. Which of the below sequencing techniques use(s) fluorescently labeled nucleotides
for identifying the nucleotide sequence of the template DNA strand?
A. Illumina AND PacBio.
B. Illumina AND Oxford Nanopore.
C. PacBio AND Ion Torrent.
D. Only Illumina.
E. All sequencing methods use fluorescently labeled nucleotides for identifying the
nucleotide sequence of the template DNA strand.
6. The below figures illustrate five cycles of Illumina sequencing. The colored spots
represent the clusters on the flow cell. What is the sequence of the DNA template
(cluster) in the top, left corner according to the figure?
A: Yellow
C: Red
G: Blue
T: Green
56
M. Eisele and M. Kappelmann-Fenzl