– It is a second-generation sequencing technology and employs a cyclic wash-andmeasure paradigm.
– It relies on the fidelity of DNA polymerase for its accuracy.
– pH change indicates how many bases were added.
Answers to Review Questions
1D; 2A; 3D; 4B; 5A; 6 GAGAC; 7 Polymerase; 8A; 9B; 10A, B, C; 11 BAEHCGDF;
12 IBBBT;
Acknowledgements We are grateful to Melinda Hutcheon, (Illumina, Inc., 5200 Illumina Way, San
Diego, CA 92122, US) for reviewing this chapter. We would also like to thank Illumina for the
material and illustrations provided to help us write this chapter.
References
1. Hood L, Rowen L. The human genome project: big science transforms biology and medicine.
Genome Med. 2013;5(9):79.
2. Goodwin S, McPherson JD, McCombie WR. Coming of age: ten years of next-generation
sequencing technologies. Nat Rev Genetics. 2016;17(6):333–51.
3. Glenn TC. Field guide to next-generation DNA sequencers. Mol Ecol Resour. 2011;11
(5):759–69.
4. Morganti S, Tarantino P, Ferraro E, D’Amico P, Viale G, Trapani D, et al. Complexity of genome
sequencing and reporting: next generation sequencing (NGS) technologies and implementation of
precision medicine in real life. Crit Rev Oncol Hematol. 2019;133:171–82.
5. Rhoads A, Au KF. PacBio sequencing and its applications. Genomics Proteomics Bioinformatics.
2015;13(5):278–89.
6. Muller CA, Boemo MA, Spingardi P, Kessler BM, Kriaucionis S, Simpson JT, et al. Capturing
the dynamics of genome replication on individual ultra-long nanopore sequence reads. Nat
Methods. 2019;16(5):429–36.
7. Wanunu M. Nanopores: a journey towards DNA sequencing. Phys Life Rev. 2012;9(2):125–58.
8. Jain M, Olsen HE, Paten B, Akeson M. The Oxford nanopore MinION: delivery of nanopore
sequencing to the genomics community. Genome Biol. 2016;17(1):239.
9. De Coster W, Strazisar M, De Rijk P. Critical length in long-read resequencing. NAR Genomics
Bioinform. 2020;2(1):lqz027.
10. McCombie WR, McPherson JD, Mardis ER. Next-generation sequencing technologies. Cold
Spring Harb Perspect Med. 2019;9(11):a033027.
58
M. Eisele and M. Kappelmann-Fenzl
– It relies on the fidelity of DNA polymerase for its accuracy.
– pH change indicates how many bases were added.
Answers to Review Questions
1D; 2A; 3D; 4B; 5A; 6 GAGAC; 7 Polymerase; 8A; 9B; 10A, B, C; 11 BAEHCGDF;
12 IBBBT;
Acknowledgements We are grateful to Melinda Hutcheon, (Illumina, Inc., 5200 Illumina Way, San
Diego, CA 92122, US) for reviewing this chapter. We would also like to thank Illumina for the
material and illustrations provided to help us write this chapter.
References
1. Hood L, Rowen L. The human genome project: big science transforms biology and medicine.
Genome Med. 2013;5(9):79.
2. Goodwin S, McPherson JD, McCombie WR. Coming of age: ten years of next-generation
sequencing technologies. Nat Rev Genetics. 2016;17(6):333–51.
3. Glenn TC. Field guide to next-generation DNA sequencers. Mol Ecol Resour. 2011;11
(5):759–69.
4. Morganti S, Tarantino P, Ferraro E, D’Amico P, Viale G, Trapani D, et al. Complexity of genome
sequencing and reporting: next generation sequencing (NGS) technologies and implementation of
precision medicine in real life. Crit Rev Oncol Hematol. 2019;133:171–82.
5. Rhoads A, Au KF. PacBio sequencing and its applications. Genomics Proteomics Bioinformatics.
2015;13(5):278–89.
6. Muller CA, Boemo MA, Spingardi P, Kessler BM, Kriaucionis S, Simpson JT, et al. Capturing
the dynamics of genome replication on individual ultra-long nanopore sequence reads. Nat
Methods. 2019;16(5):429–36.
7. Wanunu M. Nanopores: a journey towards DNA sequencing. Phys Life Rev. 2012;9(2):125–58.
8. Jain M, Olsen HE, Paten B, Akeson M. The Oxford nanopore MinION: delivery of nanopore
sequencing to the genomics community. Genome Biol. 2016;17(1):239.
9. De Coster W, Strazisar M, De Rijk P. Critical length in long-read resequencing. NAR Genomics
Bioinform. 2020;2(1):lqz027.
10. McCombie WR, McPherson JD, Mardis ER. Next-generation sequencing technologies. Cold
Spring Harb Perspect Med. 2019;9(11):a033027.
58
M. Eisele and M. Kappelmann-Fenzl