• Targeted sequencing
Targeted sequencing allows to focus on specific genes or regions of interest and thus
enables sequencing at much higher coverage levels. There are a variety of targeted
sequencing panels available, however, it is also possible to generate custom probe sets
[19]. Targeted sequencing is mainly used in molecular diagnostics utilizing the so-called
tumor panels (specific to the respective tumor entity).
• De novo sequencing
This method refers to sequence a novel genome, if there is no reference genome
available for alignment [20, 21].
This list represents only a small subset of the possible NGS applications. A detailed
exposition of the DNA, RNA, and epigenetic sequencing methods can be found under the
following links:
• DNA Sequencing Methods Collection:
– https://emea.illumina.com/content/dam/illumina-marketing/documents/products/
research_reviews/dna-sequencing-methods-review-web.pdf
– https://www.thermofisher.com/de/de/home/life-science/sequencing/dna-sequencing/
whole-genome-sequencing/whole-genome-sequencing-ion-torrent-next-generationsequencing.html
– https://www.pacb.com/applications/whole-genome-sequencing/
– https://nanoporetech.com/applications/whole-genome-sequencing
• RNA Sequencing Methods Collection:
– https://emea.illumina.com/content/dam/illumina-marketing/documents/products/
research_reviews/rna-sequencing-methods-review-web.pdf
– https://www.thermofisher.com/de/de/home/life-science/sequencing/rna-sequencing/
transcriptome-sequencing/transcriptome-sequencing-ion-torrent-next-generationsequencing.html
– https://www.pacb.com/applications/rna-sequencing/
– https://nanoporetech.com/applications/rna-sequencing
• Methylation Sequencing:
– https://emea.illumina.com/techniques/sequencing/methylation-sequencing.html
– https://www.thermofisher.com/de/de/home/life-science/sequencing/epigeneticsequencing/methylation-analysis.html
– https://www.pacb.com/applications/epigenetics/
1 Next Generation Sequencing (NGS): What Can Be Sequenced?
11
Targeted sequencing allows to focus on specific genes or regions of interest and thus
enables sequencing at much higher coverage levels. There are a variety of targeted
sequencing panels available, however, it is also possible to generate custom probe sets
[19]. Targeted sequencing is mainly used in molecular diagnostics utilizing the so-called
tumor panels (specific to the respective tumor entity).
• De novo sequencing
This method refers to sequence a novel genome, if there is no reference genome
available for alignment [20, 21].
This list represents only a small subset of the possible NGS applications. A detailed
exposition of the DNA, RNA, and epigenetic sequencing methods can be found under the
following links:
• DNA Sequencing Methods Collection:
– https://emea.illumina.com/content/dam/illumina-marketing/documents/products/
research_reviews/dna-sequencing-methods-review-web.pdf
– https://www.thermofisher.com/de/de/home/life-science/sequencing/dna-sequencing/
whole-genome-sequencing/whole-genome-sequencing-ion-torrent-next-generationsequencing.html
– https://www.pacb.com/applications/whole-genome-sequencing/
– https://nanoporetech.com/applications/whole-genome-sequencing
• RNA Sequencing Methods Collection:
– https://emea.illumina.com/content/dam/illumina-marketing/documents/products/
research_reviews/rna-sequencing-methods-review-web.pdf
– https://www.thermofisher.com/de/de/home/life-science/sequencing/rna-sequencing/
transcriptome-sequencing/transcriptome-sequencing-ion-torrent-next-generationsequencing.html
– https://www.pacb.com/applications/rna-sequencing/
– https://nanoporetech.com/applications/rna-sequencing
• Methylation Sequencing:
– https://emea.illumina.com/techniques/sequencing/methylation-sequencing.html
– https://www.thermofisher.com/de/de/home/life-science/sequencing/epigeneticsequencing/methylation-analysis.html
– https://www.pacb.com/applications/epigenetics/
1 Next Generation Sequencing (NGS): What Can Be Sequenced?
11