100
90
80
70
60
50
40
30
20
10
0
1 2 3 4 5 6 7 8 9
1 2 – 1 3
1 8 – 1 9
2 4 – 2 5
3 0 – 3 1
3 6 – 3 7
4 2 – 4 3
4 8 – 4 9
5 4 – 5 5
6 0 – 6 1
Position in read (bp)
Sequence content across all bases
%T
%C
%A
%G
6 6 – 6 7
7 2 – 7 3
7 8 – 7 9
8 6 – 8 7 9 2 – 9 3
9 8 – 9 9 1 0 6 – 1 0 7
1 1 4 – 1 1 5 1 2 2 – 1 2 3
Fig. 7.11 Per base sequence content of DNA library. (source: https://rtsf.natsci.msu.edu/)
100
90
80
70
60
50
40
30
20
10
0 1 2 3 4 5 6 7 8 9 15–19 30–34 45–49 60–64 80–84 100–104 120–124
Position in read (bp)
Sequence content across all bases
%T
%C
%A
%G
140–144 165–169 185–189 205–209 225–229 245–249
Fig. 7.12 Per base sequence content of RNA library. (source: https://rtsf.natsci.msu.edu/)
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M. Kappelmann-Fenzl