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© The Editor(s) (if applicable) and The Author(s), under exclusive license to
Springer Nature Switzerland AG 2020
H. Panwar et al. (eds.), Sustainable Agriculture Reviews 46, Sustainable
Agriculture Reviews 46, https://doi.org/10.1007/978-3-030-53024-2_5
Chapter 5
Molecular and Systems Biology
Approaches for Analyzing Drug-Tolerant
Bacterial Persister Cells
Xiangke Duan, Yang Fu, and Liang Yang
Abstract Persistence is an almost universal bacterial state, phenotypically characterized by tolerance to antibiotics. It plays a significant role in the intractability of
chronic and relapsing infections. Bacterial persisters are specialized survivors
which have entered a non or extremely slow growing, non replicating dormant state,
while being genetically identical to non tolerant kin. Forestalling the antibiotics
from inhibiting the target rather than by mutation underlies the antibiotics tolerance
of persister. More insights into the mechanisms of the persister formation can accelerate the discovery of new treatment strategies.
In this chapter, we summarized the approaches used for persister study. We first
discuss the fluorescent label based single cell approach that could be used to analyse
the native persisters within the population. We then discuss the high throughput
screening approaches, including transposon mutant library screening, knockout and
over expression library screening, where researchers could identify individual gene
involved in bacterial persistence using these approaches. And finally, we discuss the
omics approaches, including genomics, transcriptomic, proteomics and metabolomics used to understand the underlying mechanisms of persister formation.
Keywords System biology · Persistent bacterial populations · Fluorescent
indicator · High throughput screening · Genomics · Transcriptomic · Proteomics ·
Metabolomics
X. Duan · Y. Fu · L. Yang (*)
School of Medicine, Southern University of Science and Technology,
Shenzhen, Guangdong, People’s Republic of China
e-mail: yangl@sustech.edu.cn
© The Editor(s) (if applicable) and The Author(s), under exclusive license to
Springer Nature Switzerland AG 2020
H. Panwar et al. (eds.), Sustainable Agriculture Reviews 46, Sustainable
Agriculture Reviews 46, https://doi.org/10.1007/978-3-030-53024-2_5
Chapter 5
Molecular and Systems Biology
Approaches for Analyzing Drug-Tolerant
Bacterial Persister Cells
Xiangke Duan, Yang Fu, and Liang Yang
Abstract Persistence is an almost universal bacterial state, phenotypically characterized by tolerance to antibiotics. It plays a significant role in the intractability of
chronic and relapsing infections. Bacterial persisters are specialized survivors
which have entered a non or extremely slow growing, non replicating dormant state,
while being genetically identical to non tolerant kin. Forestalling the antibiotics
from inhibiting the target rather than by mutation underlies the antibiotics tolerance
of persister. More insights into the mechanisms of the persister formation can accelerate the discovery of new treatment strategies.
In this chapter, we summarized the approaches used for persister study. We first
discuss the fluorescent label based single cell approach that could be used to analyse
the native persisters within the population. We then discuss the high throughput
screening approaches, including transposon mutant library screening, knockout and
over expression library screening, where researchers could identify individual gene
involved in bacterial persistence using these approaches. And finally, we discuss the
omics approaches, including genomics, transcriptomic, proteomics and metabolomics used to understand the underlying mechanisms of persister formation.
Keywords System biology · Persistent bacterial populations · Fluorescent
indicator · High throughput screening · Genomics · Transcriptomic · Proteomics ·
Metabolomics
X. Duan · Y. Fu · L. Yang (*)
School of Medicine, Southern University of Science and Technology,
Shenzhen, Guangdong, People’s Republic of China
e-mail: yangl@sustech.edu.cn