TABLE 7.3 (Continued)
Experimental Evidence for the Responsiveness of hes1–7 and hey Genes to the Notch Pathway in Xenopus
Regulated by
Regulation/Details
Gain-of-Function
Loss-of-Function
(Dominant-Negative/Antagonist/Morpholino)
notch1
Positive.
NICD1: ↑hes5.5 (NF18, 28; microarray) ( Vasiliu et al.,
2015 ).
RBPJ
Positive in the nervous system.
A paired RBPJ binding site proximal to the TATA box is
necessary but not suff cient for neural expression in vivo
( Lamar and Kintner, 2005 ).
notch1/
Positive in the IMZ.
GR-RBPJ-VP16 or GR-NICD1 (Dex NF10): ↑IMZ hes5.5 GR-RBPJ-EnR (Dex NF10): ↓IMZ hes5.5 domain
RBPJ
domain (gastrula, ISH) ( Miazga and McLaughlin, 2009 ). (gastrula, ISH) ( Miazga and McLaughlin, 2009 ).
notch1
Positive.
NICD1: ↑hes5.6 (NF18, 28; microarray) ( Vasiliu et al., 2015 ).
notch1/
Positive during gastrulation/IMZ.
GR-RBPJ-VP16 or GR-NICD1 (Dex NF10): ↑IMZ hes5.6 GR-RBPJ-EnR (Dex NF10): ↓IMZ hes5.6 domain
RBPJ
domain (gastrula, ISH) ( Miazga and McLaughlin, 2009 ). (gastrula, ISH) ( Miazga and McLaughlin, 2009 ).
NICD1: ↑hes5.6 (mid-gastrula, RT-qPCR, NF11) ( Castro notch1 MO: ↓hes5.6; rescued by NICD1 (RT-qPCR, NF11)
Colabianchi et al., 2018 ).
( Castro Colabianchi et al., 2018 )
notch1
Positive.
NICD1: ↑hes5.7 (NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1/
Positive in the non-neural ectoderm.
NICD1: ↑hes5.10 throughout the non-neural ectoderm
RBPJ DBM: ↑hes5.10 in the inner cells of isolated
atypical response
(NF14) ( Deblandre et al., 1999 ); ↑hes5.10 (NF18, NF28, ectoderm (NF11, RNAse protection) ( Deblandre et al.,
to RBPJ.
microarray) ( Vasiliu et al., 2015 ).
1999 ).
hes7.2
hes7.1
hes6.1
hes5.10 hes5.7
hes5.6
hes5.5
Gene
notch1/
Negative in the neural plate.
NICD1: ↓hes6.1 (a positive regulator of neurogenesis) in RBPJ DBM : ↑hes6.1+ cell population in the neural plate
RBPJ
the neural plate and did not appear to affect expression
[data not shown in ( Koyano-Nakagawa et al., 2000 )].
in PSM at neurula stage ( Koyano-Nakagawa et al.,
2000 ), although some expansion in the PSM staining in
Figure 3A of this work is noticed.
notch1
Negative regulation in MHB establishment (non-canonical NICD1: ↓hes7.1 at the MHB (NF13) ( Takada et al., 2005 ). RBPJ DBM : did not affect hes7.1 expression at the MHB
pathway?).
( Takada et al., 2005 ).
notch1
Positive.
NICD1: ↑hes7.1 (NF28, microarray) ( Vasiliu et al., 2015 ).
dlc/
Positive in somitogenesis.
dlc overexpression or RBPJ-Ank: expanded hes7.2 into the RBPJ DBM : ↓hes7.2 in the somitomeric, TZ, and TBD
notch/
gaps of the somitomeric region (ISH late neurula/early
regions (ISH late neurula/early tailbud) ( Jen et al., 1999 ;
RBPJ
tailbud) ( Jen et al., 1999 ).
Peres et al., 2006 ).
dlc overexpression or NICD1: loss of the stripped hes7.2 dlc MO: ↓ hes7.2 (ISH, neurula) ( Peres et al., 2006 ).
pattern in the somitomeric region (ISH, neurula) ( Peres
et al., 2006 ).
notch1
Positive.
NICD1: ↑hes7.2 (NF28, microarray) ( Vasiliu et al., 2015 ).
86
Xenopus
Experimental Evidence for the Responsiveness of hes1–7 and hey Genes to the Notch Pathway in Xenopus
Regulated by
Regulation/Details
Gain-of-Function
Loss-of-Function
(Dominant-Negative/Antagonist/Morpholino)
notch1
Positive.
NICD1: ↑hes5.5 (NF18, 28; microarray) ( Vasiliu et al.,
2015 ).
RBPJ
Positive in the nervous system.
A paired RBPJ binding site proximal to the TATA box is
necessary but not suff cient for neural expression in vivo
( Lamar and Kintner, 2005 ).
notch1/
Positive in the IMZ.
GR-RBPJ-VP16 or GR-NICD1 (Dex NF10): ↑IMZ hes5.5 GR-RBPJ-EnR (Dex NF10): ↓IMZ hes5.5 domain
RBPJ
domain (gastrula, ISH) ( Miazga and McLaughlin, 2009 ). (gastrula, ISH) ( Miazga and McLaughlin, 2009 ).
notch1
Positive.
NICD1: ↑hes5.6 (NF18, 28; microarray) ( Vasiliu et al., 2015 ).
notch1/
Positive during gastrulation/IMZ.
GR-RBPJ-VP16 or GR-NICD1 (Dex NF10): ↑IMZ hes5.6 GR-RBPJ-EnR (Dex NF10): ↓IMZ hes5.6 domain
RBPJ
domain (gastrula, ISH) ( Miazga and McLaughlin, 2009 ). (gastrula, ISH) ( Miazga and McLaughlin, 2009 ).
NICD1: ↑hes5.6 (mid-gastrula, RT-qPCR, NF11) ( Castro notch1 MO: ↓hes5.6; rescued by NICD1 (RT-qPCR, NF11)
Colabianchi et al., 2018 ).
( Castro Colabianchi et al., 2018 )
notch1
Positive.
NICD1: ↑hes5.7 (NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1/
Positive in the non-neural ectoderm.
NICD1: ↑hes5.10 throughout the non-neural ectoderm
RBPJ DBM: ↑hes5.10 in the inner cells of isolated
atypical response
(NF14) ( Deblandre et al., 1999 ); ↑hes5.10 (NF18, NF28, ectoderm (NF11, RNAse protection) ( Deblandre et al.,
to RBPJ.
microarray) ( Vasiliu et al., 2015 ).
1999 ).
hes7.2
hes7.1
hes6.1
hes5.10 hes5.7
hes5.6
hes5.5
Gene
notch1/
Negative in the neural plate.
NICD1: ↓hes6.1 (a positive regulator of neurogenesis) in RBPJ DBM : ↑hes6.1+ cell population in the neural plate
RBPJ
the neural plate and did not appear to affect expression
[data not shown in ( Koyano-Nakagawa et al., 2000 )].
in PSM at neurula stage ( Koyano-Nakagawa et al.,
2000 ), although some expansion in the PSM staining in
Figure 3A of this work is noticed.
notch1
Negative regulation in MHB establishment (non-canonical NICD1: ↓hes7.1 at the MHB (NF13) ( Takada et al., 2005 ). RBPJ DBM : did not affect hes7.1 expression at the MHB
pathway?).
( Takada et al., 2005 ).
notch1
Positive.
NICD1: ↑hes7.1 (NF28, microarray) ( Vasiliu et al., 2015 ).
dlc/
Positive in somitogenesis.
dlc overexpression or RBPJ-Ank: expanded hes7.2 into the RBPJ DBM : ↓hes7.2 in the somitomeric, TZ, and TBD
notch/
gaps of the somitomeric region (ISH late neurula/early
regions (ISH late neurula/early tailbud) ( Jen et al., 1999 ;
RBPJ
tailbud) ( Jen et al., 1999 ).
Peres et al., 2006 ).
dlc overexpression or NICD1: loss of the stripped hes7.2 dlc MO: ↓ hes7.2 (ISH, neurula) ( Peres et al., 2006 ).
pattern in the somitomeric region (ISH, neurula) ( Peres
et al., 2006 ).
notch1
Positive.
NICD1: ↑hes7.2 (NF28, microarray) ( Vasiliu et al., 2015 ).
86
Xenopus