85
hes5.4
hes5.3
hes5.2
hes5.1
dll1/
Positive.
notch1/
An HDAC inhibitor enhanced hes5.1 response to induction
RBPJ/
by dll1 in neuralized animal caps, supporting the
maml1
hypothesis that activation by Dll1/Notch disrupts the
formation of the repressor complex containing RBPJ
and HDAC-1 that maintains Notch-target genes
repressed in the absence of Notch signaling ( Kao et al.,
1998 ).
Paired RBPJ binding site proximal to the TATA box,
necessary but not suff cient for neural expression in vivo
( Lamar and Kintner, 2005 ).
jag1
Positive during PN.
NICD1: ↑hes5.1 in animal caps (NF10.5, sqRT-PCR)
( Kinoshita et al., 2011) and whole embryos (NF18, 28,
microarray) ( Vasiliu et al., 2015 ). (RT-qPCR, sometime
between NF9.5–10) ( Mir et al., 2008 ). Induced ectopic
hes5.1 in neural and non-neural ectoderm (neural plate
stage) ( Deblandre et al., 1999 ).
dll1 overexpression , RBPJ-Ank or NICD1 but not RBPJ:
↑hes5.1 in neuralized or naive animal caps (neurula
stage) ( Wettstein et al., 1997 ; Lamar et al., 2001 ; Lahaye
et al., 2002 ; Pichon et al., 2002 ). The RAM23 domain
and ankyrin repeats (Ank) of NICD1 were essential for
this induction, with some contribution of sequences
downstream of the Ank repeats ( Wettstein et al., 1997 ).
hGR-NICD1(22) or GR-RBPJ-Ank (Dex 1 hr.) +/− CHX:
↑hes5.1 in neuralized animal caps ( Wettstein et al.,
1997 ).
hGR-NICD1(22) or hGR-RBPJ-Ank, Dex 30 min: ↑hes5.1
in neuralized animal caps ( Lahaye et al., 2002 ).
hGR-RBPJ-Ank, Dex 1 hr +/− CHX : ↑hes5.1 in naive
animal caps ( Lahaye et al., 2002 ).
RBPJ.S v2-Ank ↑ hes5.1 in naive animal caps (RT-sqPCR)
( Ito et al., 2007b ).
RBPJ overexpression: inhibited hes5.1 induction by dll1 in
neuralized animal caps (similar to RBPJ DBM)
( Wettstein et al., 1997 ).
jag1 overexpression but not jag1ICD: ↑ hes5.1 in
neuralized animal caps ( Kiyota and Kinoshita, 2004 ).
RBPJ DBM : suppressed NICD1’s ability to induce hes5.1 in
naive animal caps (neurula stage) ( Lahaye et al., 2002 ;
Pichon et al., 2002 ) and dll1’s or neurog2’s ability to
induce hes5.1 in neuralized animal caps ( Wettstein et al.,
1997 ; Lamar et al., 2001 ). hes5.1 was downregulated in
embryos derived from RBPJ DBM-injected oocytes
(RT-qPCR, sometime between NF9.5–10) ( Mir et al.,
2008 ).
RBPJ.S v2 DBM : ↓hes5.1 in naive animal caps (RT-sqPCR)
( Ito et al., 2007b ).
DN-maml1 : ↓hes5.1 in domains of PN ( Katada and
Kinoshita, 2003 ).
notch1/
Positive in the ectoderm/neuroectoderm.
NICD1: induced ectopic hes5.2 in neural and non-neural
RBPJ
Paired RBPJ binding site proximal to the TATA box
ectoderm ( Deblandre et al., 1999 ): ↑hes5.2 in neuralized
( Lamar and Kintner, 2005 ).
animal caps (NF14–15) ( Sölter et al., 2006 ) and whole
embryos (NF18, NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1
Positive.
NICD1: ↑hes5.3 (NF18, NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1/
Positive in placodes.
NICD1: ↑hes5.4 in placodes and adjacent non-neural
RBPJ DBM : ↓hes5.4 in neural plate and placodes (ISH,
RBPJ
ectoderm, even in the absence of function of the PPE
neural plate stage) ( Riddiford and Schlosser, 2017 ).
genes six1/eya1 (ISH, neural plate stage) ( Riddiford and
Schlosser, 2017 ).
(Continued)
Notch Signaling in Early Embryogenesis
hes5.4
hes5.3
hes5.2
hes5.1
dll1/
Positive.
notch1/
An HDAC inhibitor enhanced hes5.1 response to induction
RBPJ/
by dll1 in neuralized animal caps, supporting the
maml1
hypothesis that activation by Dll1/Notch disrupts the
formation of the repressor complex containing RBPJ
and HDAC-1 that maintains Notch-target genes
repressed in the absence of Notch signaling ( Kao et al.,
1998 ).
Paired RBPJ binding site proximal to the TATA box,
necessary but not suff cient for neural expression in vivo
( Lamar and Kintner, 2005 ).
jag1
Positive during PN.
NICD1: ↑hes5.1 in animal caps (NF10.5, sqRT-PCR)
( Kinoshita et al., 2011) and whole embryos (NF18, 28,
microarray) ( Vasiliu et al., 2015 ). (RT-qPCR, sometime
between NF9.5–10) ( Mir et al., 2008 ). Induced ectopic
hes5.1 in neural and non-neural ectoderm (neural plate
stage) ( Deblandre et al., 1999 ).
dll1 overexpression , RBPJ-Ank or NICD1 but not RBPJ:
↑hes5.1 in neuralized or naive animal caps (neurula
stage) ( Wettstein et al., 1997 ; Lamar et al., 2001 ; Lahaye
et al., 2002 ; Pichon et al., 2002 ). The RAM23 domain
and ankyrin repeats (Ank) of NICD1 were essential for
this induction, with some contribution of sequences
downstream of the Ank repeats ( Wettstein et al., 1997 ).
hGR-NICD1(22) or GR-RBPJ-Ank (Dex 1 hr.) +/− CHX:
↑hes5.1 in neuralized animal caps ( Wettstein et al.,
1997 ).
hGR-NICD1(22) or hGR-RBPJ-Ank, Dex 30 min: ↑hes5.1
in neuralized animal caps ( Lahaye et al., 2002 ).
hGR-RBPJ-Ank, Dex 1 hr +/− CHX : ↑hes5.1 in naive
animal caps ( Lahaye et al., 2002 ).
RBPJ.S v2-Ank ↑ hes5.1 in naive animal caps (RT-sqPCR)
( Ito et al., 2007b ).
RBPJ overexpression: inhibited hes5.1 induction by dll1 in
neuralized animal caps (similar to RBPJ DBM)
( Wettstein et al., 1997 ).
jag1 overexpression but not jag1ICD: ↑ hes5.1 in
neuralized animal caps ( Kiyota and Kinoshita, 2004 ).
RBPJ DBM : suppressed NICD1’s ability to induce hes5.1 in
naive animal caps (neurula stage) ( Lahaye et al., 2002 ;
Pichon et al., 2002 ) and dll1’s or neurog2’s ability to
induce hes5.1 in neuralized animal caps ( Wettstein et al.,
1997 ; Lamar et al., 2001 ). hes5.1 was downregulated in
embryos derived from RBPJ DBM-injected oocytes
(RT-qPCR, sometime between NF9.5–10) ( Mir et al.,
2008 ).
RBPJ.S v2 DBM : ↓hes5.1 in naive animal caps (RT-sqPCR)
( Ito et al., 2007b ).
DN-maml1 : ↓hes5.1 in domains of PN ( Katada and
Kinoshita, 2003 ).
notch1/
Positive in the ectoderm/neuroectoderm.
NICD1: induced ectopic hes5.2 in neural and non-neural
RBPJ
Paired RBPJ binding site proximal to the TATA box
ectoderm ( Deblandre et al., 1999 ): ↑hes5.2 in neuralized
( Lamar and Kintner, 2005 ).
animal caps (NF14–15) ( Sölter et al., 2006 ) and whole
embryos (NF18, NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1
Positive.
NICD1: ↑hes5.3 (NF18, NF28, microarray) ( Vasiliu et al.,
2015 ).
notch1/
Positive in placodes.
NICD1: ↑hes5.4 in placodes and adjacent non-neural
RBPJ DBM : ↓hes5.4 in neural plate and placodes (ISH,
RBPJ
ectoderm, even in the absence of function of the PPE
neural plate stage) ( Riddiford and Schlosser, 2017 ).
genes six1/eya1 (ISH, neural plate stage) ( Riddiford and
Schlosser, 2017 ).
(Continued)
Notch Signaling in Early Embryogenesis