Gene
TABLE 7.3 (Continued)
Experimental Evidence for the Responsiveness of hes1–7 and hey Genes to the Notch Pathway in Xenopus
Regulated by
Regulation/Details
Gain-of-Function
Loss-of-Function
(Dominant-Negative/Antagonist/Morpholino)
dlc/notch/
Positive in somitogenesis.
RBPJ
dlc and hes4: complementary expression patterns in
somitomeres, consistent with an inductive role of dlc on
hes4 through Notch activation in neighboring cells ( Jen
et al., 1997 ).
notch/
Positive.
RBPJ
A paired RBPJ motif in the proximal promoter and a
hes4 3’UTR
minimal 25 bp sequence in the 3’UTR, are necessary for
hes4 expression in neural tissue, pronephros, and
anterior PSM, but are insuff cient for hes4 expression in
the FP.
3
′ UTR confers global instability to hes4 mRNA, except in
the anterior PSM. In vivo gene reporter, tailbud stage
( Davis et al., 2001 ).
Notch1 is associated with the RBPJ site of the hes4
genomic loci (ChIP assay, NF25) ( Sakano et al., 2010 ).
dll1/
Positive in DML precursors during gastrulation.
NICD1: ↑hes4+ population of FP precursors in the GO and hes4
notch1/
FP domain in neurulae ( López et al., 2005 ).
RBPJ
dll1 overexpression :↑hes4+ population of FP precursors in
the GO ( López et al., 2005 ).
dll1/
Positive in ectoderm.
NICD1: ↑hes4 in animal caps (RT-PCR, NF20) and
notch1
cell-autonomously in ectoderm (ISH, NF20) ( Cui,
2005 ).
dlc-tr or RBPJ DBM : suppressed the segmental hes4
prepattern in somitomeres ( Jen et al., 1997 ).
dlc MO: ↓hes4 (ISH, tailbud stage) ( Peres et al., 2006 ).
RBPJDBM: ↓hes4+ population of FP precursors in the GO
and hes4 FP in neurulae ( López et al., 2005 ).
dll1 STU : prevented the induction of hes4 by neurog2 in
animal caps (RT-PCR, NF20) ( Cui, 2005 ).
84
Xenopus