220
Xenopus
mediated targeted integration in Xenopus tropicalis. The
FASEB Journal 29, 4914–4923.
Shi, Z., Xin, H., Tian, D., Lian, J., Wang, J., Liu, G., Ran, R., Shi,
S., Zhang, Z., Shi, Y., et al. (2019). Modeling human point
mutation diseases in Xenopus tropicalis with a modif ed
CRISPR/Cas9 system. FASEB Journal: Offcial Publication
of the Federation of American Societies for Experimental
Biology 33, 6962–6968.
Shibano, T., Takeda, M., Suetake, I., Kawakami, K., Asashima, M.,
Tajima, S., and Taira, M. (2007). Recombinant Tol2 transposase with activity in Xenopus embryos. Febs Lett 581,
4333–4336.
Shibata, Y., Okada, M., Miller, T.C., and Shi, Y.-B. (2019).
Knocking out histone methyltransferase PRMT1 leads to
stalled tadpole development and lethality in Xenopus tropicalis. Biochimica Et Biophysica Acta Bba: Gen Subj 1864,
129482.
Shibata, Y., Tanizaki, Y., and Shi, Y.-B. (2020a). Thyroid hormone
receptor beta is critical for intestinal remodeling during
Xenopus tropicalis metamorphosis. Cell Biosci 10, 46.
Shibata, Y., Wen, L., Okada, M., and Shi, Y.-B. (2020b). Organspecifc requirements for thyroid hormone receptor ensure
temporal coordination of tissue-specif c transformations
and completion of Xenopus metamorphosis. Thyroid 30,
300–313.
Sinzelle, L., Vallin, J., Coen, L., Chesneau, A., Pasquier, D.D.,
Pollet, N., Demeneix, B., and Mazabraud, A. (2006).
Generation of trangenic Xenopus laevis using the Sleeping
Beauty transposon system. Transgenic Res 15, 751–760.
Smith, S.J., Towers, N., Demetriou, K., and Mohun, T.J. (2020).
Defective heart chamber growth and myof brillogenesis after
knockout of adprhl1 gene function by targeted disruption of
the ancestral catalytic active site. PLoS One 15, e0235433.
Sparrow, D.B., Latinkic, B., and Mohun, T.J. (2000). A simplif ed
method of generating transgenic Xenopus. Nucleic Acids Res
28, 12e–112.
Square, T.A., Jandzik, D., Massey, J.L., Romášek, M., Stein, H.P.,
Hansen, A.W., Purkayastha, A., Cattell, M.V., and Medeiros,
D.M. (2020). Evolution of the endothelin pathway drove
neural crest cell diversif cation. Nature 585, 563–568.
Steimle, J.D., Rankin, S.A., Slagle, C.E., Bekeny, J., Rydeen, A.B.,
Chan, S.S.-K., Kweon, J., Yang, X.H., Ikegami, K., Nadadur,
R.D., et al. (2018). Evolutionarily conserved Tbx5-Wnt2/2b
pathway orchestrates cardiopulmonary development. Proc
National Acad Sci 115, E10615–E10624 .
Stemple, D.L. (2004). TILLING: A high-throughput harvest for
functional genomics. Nat Rev Genet 5, 145–150.
Sterner, Z.R., Rankin, S.A., Wlizla, M., Choi, J.A., Luedeke, D.M.,
Zorn, A.M., and Buchholz, D.R. (2019). Novel vectors for
functional interrogation of Xenopus ORFeome coding
sequences. Genesis 57, e23329.
Sterner, Z.R., Shewade, L.H., Mertz, K.M., Sturgeon, S.M., and
Buchholz, D.R. (2020). Glucocorticoid receptor is required
to survive through metamorphosis in the frog Xenopus tropicalis. Gen Comp Endocr 291, 113419.
Sullivan, C.H., Majumdar, H.D., Neilson, K.M., and Moody, S.A.
(2019). Six1 and Irx1 have reciprocal interactions during
cranial placode and otic vesicle formation. Developmental
Biology 446, 68–79.
Suzuki, K.-I.T., Isoyama, Y., Kashiwagi, K., Sakuma, T., Ochiai, H.,
Sakamoto, N., Furuno, N., Kashiwagi, A., and Yamamoto, T.
(2013). High effciency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
Biology Open 2, 448–452.
Suzuki, M., Takagi, C., Miura, S., Sakane, Y., Suzuki, M., Sakuma,
T., Sakamoto, N., Endo, T., Kamei, Y., Sato, Y., et al. (2016).
In vivo tracking of histone H3 lysine 9 acetylation in Xenopus
laevis during tail regeneration. Genes Cells 21, 358–369.
Szenker-Ravi, E., Altunoglu, U., Leushacke, M., Bosso-Lefèvre,
C., Khatoo, M., Tran, H.T., Naert, T., Noelanders, R.,
Hajamohideen, A., Beneteau, C., et al. (2018). RSPO2 inhibition of RNF43 and ZNRF3 governs limb development
independently of LGR4/5/6. Nature 557, 564–569.
Takagi, C., Sakamaki, K., Morita, H., Hara, Y., Suzuki, M.,
Kinoshita, N., and Ueno, N. (2013). Transgenic Xenopus
laevis for live imaging in cell and developmental biology.
Development, Growth & Differentiation 55, 422–433.
Tandon, P., Conlon, F., Furlow, J.D., and Horb, M.E. (2017).
Expanding the genetic toolkit in Xenopus: Approaches and
opportunities for human disease modeling. Developmental
Biology 426, 325–335.
Tanizaki, Y., Bao, L., Shi, B., and Shi, Y.-B. (2021a). A role of
endogenous histone acetyltransferase steroid hormone
receptor coactivator 3 in thyroid hormone signaling during
Xenopus intestinal metamorphosis. Thyroid 31, 692–702.
Tanizaki, Y., Shibata, Y., Zhang, H., and Shi, Y.-B. (2021b).
Analysis of thyroid hormone receptor α-knockout tadpoles
reveals that the activation of cell cycle program is involved
in thyroid hormone-induced larval epithelial cell death and
adult intestinal stem cell development during Xenopus tropicalis metamorphosis. Thyroid 31, 128–142.
Thyagarajan, B., Olivares, E.C., Hollis, R.P., Ginsburg, D.S., and
Calos, M.P. (2001). Site-specifc genomic integration in
mammalian cells mediated by phage φC31 integrase. Mol
Cell Biol 21, 3926–3934.
Tompkins, R. (1978). Triploid and gynogenetic diploid Xenopus
laevis. J Exp Zool 203, 251–255.
Tran, H.T., and Vleminckx, K. (2014). Design and use of transgenic
reporter strains for detecting activity of signaling pathways
in Xenopus. Methods (San Diego, Calif) 66, 422–432.
Viet, J., Reboutier, D., Hardy, S., Lachke, S.A., Paillard, L., and
Gautier-Courteille, C. (2020). Modeling ocular lens disease
in Xenopus. Dev Dynam 249, 610–621.
Vogt, J., Traynor, R., and Sapkota, G.P. (2011). The specif cities of
small molecule inhibitors of the TGFß and BMP pathways.
Cell Signal 23, 1831–1842.
Waldner, C., Sakamaki, K., Ueno, N., Turan, G., and Ryffel, G.U.
(2006). Transgenic Xenopus laevis strain expressing cre
recombinase in muscle cells. Developmental Dynamics:
An Offcial Publication of the American Association of
Anatomists 235, 2220–2228.
Wallace, H. (1960). The development of anucleolate embryos of
Xenopus laevis. J Embryol Exp Morph 8, 405–413.
Wang, C., Qi, X., Zhou, X., Sun, J., Cai, D., Lu, G., Chen, X.,
Jiang, Z., Yao, Y., Chan, W.Y., et al. (2020). RNA-Seq analysis on ets1 mutant embryos of Xenopus tropicalis identif es
microseminoprotein beta gene 3 as an essential regulator of
neural crest migration. Faseb J 34, 12726–12738.
Wang, F., Shi, Z., Cui, Y., Guo, X., Shi, Y.-B., and Chen, Y. (2015).
Targeted gene disruption in Xenopus laevis using CRISPR/
Cas9. Cell & Bioscience 5, 15.
Waqas, S.F.H., Noble, A., Hoang, A.C., Ampem, G., Popp, M.,
Strauß, S., Guille, M., and Röszer, T. (2017). Adipose tissue macrophages develop from bone marrow: Independent
progenitors in Xenopus laevis and mouse. J Leukocyte Biol
102, 845–855.
Wen, L., Fu, L., Guo, X., Chen, Y., and Shi, Y.-B. (2015). Histone
methyltransferase Dot1L plays a role in postembryonic
Xenopus
mediated targeted integration in Xenopus tropicalis. The
FASEB Journal 29, 4914–4923.
Shi, Z., Xin, H., Tian, D., Lian, J., Wang, J., Liu, G., Ran, R., Shi,
S., Zhang, Z., Shi, Y., et al. (2019). Modeling human point
mutation diseases in Xenopus tropicalis with a modif ed
CRISPR/Cas9 system. FASEB Journal: Offcial Publication
of the Federation of American Societies for Experimental
Biology 33, 6962–6968.
Shibano, T., Takeda, M., Suetake, I., Kawakami, K., Asashima, M.,
Tajima, S., and Taira, M. (2007). Recombinant Tol2 transposase with activity in Xenopus embryos. Febs Lett 581,
4333–4336.
Shibata, Y., Okada, M., Miller, T.C., and Shi, Y.-B. (2019).
Knocking out histone methyltransferase PRMT1 leads to
stalled tadpole development and lethality in Xenopus tropicalis. Biochimica Et Biophysica Acta Bba: Gen Subj 1864,
129482.
Shibata, Y., Tanizaki, Y., and Shi, Y.-B. (2020a). Thyroid hormone
receptor beta is critical for intestinal remodeling during
Xenopus tropicalis metamorphosis. Cell Biosci 10, 46.
Shibata, Y., Wen, L., Okada, M., and Shi, Y.-B. (2020b). Organspecifc requirements for thyroid hormone receptor ensure
temporal coordination of tissue-specif c transformations
and completion of Xenopus metamorphosis. Thyroid 30,
300–313.
Sinzelle, L., Vallin, J., Coen, L., Chesneau, A., Pasquier, D.D.,
Pollet, N., Demeneix, B., and Mazabraud, A. (2006).
Generation of trangenic Xenopus laevis using the Sleeping
Beauty transposon system. Transgenic Res 15, 751–760.
Smith, S.J., Towers, N., Demetriou, K., and Mohun, T.J. (2020).
Defective heart chamber growth and myof brillogenesis after
knockout of adprhl1 gene function by targeted disruption of
the ancestral catalytic active site. PLoS One 15, e0235433.
Sparrow, D.B., Latinkic, B., and Mohun, T.J. (2000). A simplif ed
method of generating transgenic Xenopus. Nucleic Acids Res
28, 12e–112.
Square, T.A., Jandzik, D., Massey, J.L., Romášek, M., Stein, H.P.,
Hansen, A.W., Purkayastha, A., Cattell, M.V., and Medeiros,
D.M. (2020). Evolution of the endothelin pathway drove
neural crest cell diversif cation. Nature 585, 563–568.
Steimle, J.D., Rankin, S.A., Slagle, C.E., Bekeny, J., Rydeen, A.B.,
Chan, S.S.-K., Kweon, J., Yang, X.H., Ikegami, K., Nadadur,
R.D., et al. (2018). Evolutionarily conserved Tbx5-Wnt2/2b
pathway orchestrates cardiopulmonary development. Proc
National Acad Sci 115, E10615–E10624 .
Stemple, D.L. (2004). TILLING: A high-throughput harvest for
functional genomics. Nat Rev Genet 5, 145–150.
Sterner, Z.R., Rankin, S.A., Wlizla, M., Choi, J.A., Luedeke, D.M.,
Zorn, A.M., and Buchholz, D.R. (2019). Novel vectors for
functional interrogation of Xenopus ORFeome coding
sequences. Genesis 57, e23329.
Sterner, Z.R., Shewade, L.H., Mertz, K.M., Sturgeon, S.M., and
Buchholz, D.R. (2020). Glucocorticoid receptor is required
to survive through metamorphosis in the frog Xenopus tropicalis. Gen Comp Endocr 291, 113419.
Sullivan, C.H., Majumdar, H.D., Neilson, K.M., and Moody, S.A.
(2019). Six1 and Irx1 have reciprocal interactions during
cranial placode and otic vesicle formation. Developmental
Biology 446, 68–79.
Suzuki, K.-I.T., Isoyama, Y., Kashiwagi, K., Sakuma, T., Ochiai, H.,
Sakamoto, N., Furuno, N., Kashiwagi, A., and Yamamoto, T.
(2013). High effciency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos.
Biology Open 2, 448–452.
Suzuki, M., Takagi, C., Miura, S., Sakane, Y., Suzuki, M., Sakuma,
T., Sakamoto, N., Endo, T., Kamei, Y., Sato, Y., et al. (2016).
In vivo tracking of histone H3 lysine 9 acetylation in Xenopus
laevis during tail regeneration. Genes Cells 21, 358–369.
Szenker-Ravi, E., Altunoglu, U., Leushacke, M., Bosso-Lefèvre,
C., Khatoo, M., Tran, H.T., Naert, T., Noelanders, R.,
Hajamohideen, A., Beneteau, C., et al. (2018). RSPO2 inhibition of RNF43 and ZNRF3 governs limb development
independently of LGR4/5/6. Nature 557, 564–569.
Takagi, C., Sakamaki, K., Morita, H., Hara, Y., Suzuki, M.,
Kinoshita, N., and Ueno, N. (2013). Transgenic Xenopus
laevis for live imaging in cell and developmental biology.
Development, Growth & Differentiation 55, 422–433.
Tandon, P., Conlon, F., Furlow, J.D., and Horb, M.E. (2017).
Expanding the genetic toolkit in Xenopus: Approaches and
opportunities for human disease modeling. Developmental
Biology 426, 325–335.
Tanizaki, Y., Bao, L., Shi, B., and Shi, Y.-B. (2021a). A role of
endogenous histone acetyltransferase steroid hormone
receptor coactivator 3 in thyroid hormone signaling during
Xenopus intestinal metamorphosis. Thyroid 31, 692–702.
Tanizaki, Y., Shibata, Y., Zhang, H., and Shi, Y.-B. (2021b).
Analysis of thyroid hormone receptor α-knockout tadpoles
reveals that the activation of cell cycle program is involved
in thyroid hormone-induced larval epithelial cell death and
adult intestinal stem cell development during Xenopus tropicalis metamorphosis. Thyroid 31, 128–142.
Thyagarajan, B., Olivares, E.C., Hollis, R.P., Ginsburg, D.S., and
Calos, M.P. (2001). Site-specifc genomic integration in
mammalian cells mediated by phage φC31 integrase. Mol
Cell Biol 21, 3926–3934.
Tompkins, R. (1978). Triploid and gynogenetic diploid Xenopus
laevis. J Exp Zool 203, 251–255.
Tran, H.T., and Vleminckx, K. (2014). Design and use of transgenic
reporter strains for detecting activity of signaling pathways
in Xenopus. Methods (San Diego, Calif) 66, 422–432.
Viet, J., Reboutier, D., Hardy, S., Lachke, S.A., Paillard, L., and
Gautier-Courteille, C. (2020). Modeling ocular lens disease
in Xenopus. Dev Dynam 249, 610–621.
Vogt, J., Traynor, R., and Sapkota, G.P. (2011). The specif cities of
small molecule inhibitors of the TGFß and BMP pathways.
Cell Signal 23, 1831–1842.
Waldner, C., Sakamaki, K., Ueno, N., Turan, G., and Ryffel, G.U.
(2006). Transgenic Xenopus laevis strain expressing cre
recombinase in muscle cells. Developmental Dynamics:
An Offcial Publication of the American Association of
Anatomists 235, 2220–2228.
Wallace, H. (1960). The development of anucleolate embryos of
Xenopus laevis. J Embryol Exp Morph 8, 405–413.
Wang, C., Qi, X., Zhou, X., Sun, J., Cai, D., Lu, G., Chen, X.,
Jiang, Z., Yao, Y., Chan, W.Y., et al. (2020). RNA-Seq analysis on ets1 mutant embryos of Xenopus tropicalis identif es
microseminoprotein beta gene 3 as an essential regulator of
neural crest migration. Faseb J 34, 12726–12738.
Wang, F., Shi, Z., Cui, Y., Guo, X., Shi, Y.-B., and Chen, Y. (2015).
Targeted gene disruption in Xenopus laevis using CRISPR/
Cas9. Cell & Bioscience 5, 15.
Waqas, S.F.H., Noble, A., Hoang, A.C., Ampem, G., Popp, M.,
Strauß, S., Guille, M., and Röszer, T. (2017). Adipose tissue macrophages develop from bone marrow: Independent
progenitors in Xenopus laevis and mouse. J Leukocyte Biol
102, 845–855.
Wen, L., Fu, L., Guo, X., Chen, Y., and Shi, Y.-B. (2015). Histone
methyltransferase Dot1L plays a role in postembryonic