218
Xenopus
Kim, D., An, H., Shearer, R.S., Sharif, M., Fan, C., Choi, J., Ryu,
S., and Park, Y. (2019). A principled strategy for mapping
enhancers to genes. Sci Rep-Uk 9, 11043.
Kim, S.K., Brotslaw, E., Thome, V., Mitchell, J., Ventrella, R.,
Collins, C., and Mitchell, B. (2021). A role for Cep70 in
centriole amplifcation in multiciliated cells. Dev Biol 471,
10–17.
Klein, S.L., Strausberg, R.L., Wagner, L., Pontius, J., Clifton, S.W.,
and Richardson, P. (2002). Genetic and genomic tools for
Xenopus research: The NIH Xenopus initiative. Null 225,
384–391.
Kroll, K.L., and Amaya, E. (1996). Transgenic Xenopus embryos
from sperm nuclear transplantations reveal FGF signaling
requirements during gastrulation. Development (Cambridge,
England) 122, 3173–3183.
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy,
M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P.,
and Chien, C. (2007). The Tol2kit: A multisite gatewaybased construction kit for Tol2 transposon transgenesis constructs. Dev Dynam 236, 3088–3099.
Landim-Vieira, M., Johnston, J.R., Ji, W., Mis, E.K., Tijerino, J.,
Spencer-Manzon, M., Jeffries, L., Hall, E.K., PaniselloManterola, D., Khokha, M.K., et al. (2020). Familial dilated
cardiomyopathy associated with a novel combination of compound heterozygous TNNC1 variants. Front Physiol 10 , 1612.
Lane, M.A., Kimber, M., and Khokha, M.K. (2013). Breeding based
remobilization of Tol2 transposon in Xenopus tropicalis.
PLoS One 8, e76807.
Lei, Y., Guo, X., Deng, Y., Chen, Y., and Zhao, H. (2013).
Generation of gene disruptions by transcription activator-like
effector nucleases (TALENs) in Xenopus tropicalis embryos.
Cell & Bioscience 3, 21.
Lei, Y., Guo, X., Liu, Y., Cao, Y., Deng, Y., Chen, X., Cheng, C.H.K.,
Dawid, I.B., Chen, Y., and Zhao, H. (2012). Eff cient targeted gene disruption in Xenopus embryos using engineered
transcription activator-like effector nucleases (TALENs).
Proceedings of the National Academy of Sciences of the
United States of America 109, 17484–17489.
Li, Y.E., Allen, B.G., and Weeks, D.L. (2012). Xenopus protocols, post-genomic approaches. Methods Mol Biology 917,
219–230.
Liu, Z., Cheng, T.T.K., Shi, Z., Liu, Z., Lei, Y., Wang, C., Shi, W.,
Chen, X., Qi, X., Cai, D., et al. (2016). Eff cient genome
editing of genes involved in neural crest development using
the CRISPR/Cas9 system in Xenopus embryos. Cell &
Bioscience 6, 22.
Love, N.R., Chen, Y., Ishibashi, S., Kritsiligkou, P., Lea, R., Koh, Y.,
Gallop, J.L., Dorey, K., and Amaya, E. (2013). Amputationinduced reactive oxygen species are required for successful
Xenopus tadpole tail regeneration. Nature Publishing Group
15, 222–228.
Love, N., Thuret, R., Chen, Y., Ishibashi, S., Sabherwal, N.,
Paredes, R., Alves-Silva, J., Dorey, K., Noble, A.M., Guille,
M.J., et al. (2011a). pTransgenesis: A cross-species, modular
transgenesis resource. Development (Cambridge, England)
138, 5451–5458.
Love, N.R., Thuret, R., Chen, Y., Ishibashi, S., Sabherwal, N.,
Paredes, R., Alves-Silva, J., Dorey, K., Noble, A.M., Guille,
M.J., et al. (2011b). pTransgenesis: A cross-species, modular
transgenesis resource. Development 138, 5451–5458.
Mao, C.-Z., Zheng, L., Zhou, Y.-M., Wu, H.-Y., Xia, J.-B., Liang,
C.-Q., Guo, X.-F., Peng, W.-T., Zhao, H., Cai, W.-B., et al.
(2018). CRISPR/Cas9-mediated effcient and precise targeted
integration of donor DNA harboring double cleavage sites in
Xenopus tropicalis. The FASEB Journal 32, 6495–6509.
Marquez, J., Mann, N., Arana, K., Deniz, E., Ji, W., Konstantino,
M., Mis, E.K., Deshpande, C., Jeffries, L., McGlynn, J., et al.
(2021). DLG5 variants are associated with multiple congenital anomalies including ciliopathy phenotypes. J Med Genet
58, 453–464.
Marsh-Armstrong, N., Cai, L., and Brown, D.D. (2004). Thyroid
hormone controls the development of connections between
the spinal cord and limbs during Xenopus laevis metamorphosis. Proc National Acad Sci 101, 165–170.
Marsh-Armstrong, N., Huang, H., Berry, D.L., and Brown, D.D.
(1999). Germ-line transmission of transgenes in Xenopus
laevis. Proc National Acad Sci 96, 14389–14393.
Miyamoto, K., Suzuki, K.T., Suzuki, M., Sakane, Y., Sakuma, T.,
Herberg, S., Simeone, A., Simpson, D., Jullien, J., Yamamoto,
T., et al. (2015). The expression of TALEN before fertilization provides a rapid knock-out phenotype in Xenopus laevis
founder embryos. PLoS One 10, e0142946.
Mukhi, S., and Brown, D.D. (2011). Transdifferentiation of tadpole
pancreatic acinar cells to duct cells mediated by Notch and
stromelysin-3. Developmental Biology 351, 311–317.
Mukhi, S., Cai, L., and Brown, D.D. (2010). Gene switching at
Xenopus laevis metamorphosis. Developmental Biology 338,
117–126.
Mukhi, S., Horb, M.E., and Brown, D.D. (2009). Remodeling of
insulin producing beta-cells during Xenopus laevis metamorphosis. Developmental Biology 328, 384–391.
Mukhi, S., Mao, J., and Brown, D.D. (2008). Remodeling the
exocrine pancreas at metamorphosis in Xenopus laevis.
Proceedings of the National Academy of Sciences of the
United States of America 105, 8962–8967.
Naert, T., Colpaert, R., Nieuwenhuysen, T.V., Dimitrakopoulou, D.,
Leoen, J., Haustraete, J., Boel, A., Steyaert, W., Lepez, T.,
Deforce, D., et al. (2016). CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis. Scientif c Reports
6, 35264.
Naert, T., Dimitrakopoulou, D., Tulkens, D., Demuynck, S., Carron,
M., Noelanders, R., Eeckhout, L., Isterdael, G.V., Deforce,
D., Vanhove, C., et al. (2020a). RBL1 (p107) functions as
tumor suppressor in glioblastoma and small-cell pancreatic
neuroendocrine carcinoma in Xenopus tropicalis. Oncogene
39, 2692–2706.
Naert, T., Tulkens, D., Edwards, N.A., Carron, M., Shaidani, N.-I.,
Wlizla, M., Boel, A., Demuynck, S., Horb, M.E., Coucke,
P., et al. (2020b). Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in
Xenopus and zebrafsh embryos. Sci Rep-Uk 10, 14662.
Nakade, S., Sakuma, T., Sakane, Y., Hara, Y., Kurabayashi, A.,
Kashiwagi, K., Kashiwagi, A., Yamamoto, T., and Obara, M.
(2015). Homeolog-specifc targeted mutagenesis in Xenopus
laevis using TALENs. In Vitro Cellular & Developmental
Biology: Animal 51, 879–884.
Nakade, S., Tsubota, T., Sakane, Y., Kume, S., Sakamoto, N., Obara,
M., Daimon, T., Sezutsu, H., Yamamoto, T., Sakuma, T., et al.
(2014). Microhomology-mediated end-joining-dependent
integration of donor DNA in cells and animals using TALENs
and CRISPR/Cas9. Nature Communications 5, 5560.
Nakai, Y., Nakajima, K., Robert, J., and Yaoita, Y. (2016). Ouro
proteins are not essential to tail regression during Xenopus
tropicalis metamorphosis. Genes Cells 21, 275–286.
Nakajima, K., Nakai, Y., Okada, M., and Yaoita, Y. (2013). Targeted
gene disruption in the Xenopus tropicalis genome using
designed TALE nucleases. Zool Sci 30, 455–460.
Nakajima, K., Nakajima, T., Takase, M., and Yaoita, Y. (2012).
Generation of albino Xenopus tropicalis using zinc-f nger
Xenopus
Kim, D., An, H., Shearer, R.S., Sharif, M., Fan, C., Choi, J., Ryu,
S., and Park, Y. (2019). A principled strategy for mapping
enhancers to genes. Sci Rep-Uk 9, 11043.
Kim, S.K., Brotslaw, E., Thome, V., Mitchell, J., Ventrella, R.,
Collins, C., and Mitchell, B. (2021). A role for Cep70 in
centriole amplifcation in multiciliated cells. Dev Biol 471,
10–17.
Klein, S.L., Strausberg, R.L., Wagner, L., Pontius, J., Clifton, S.W.,
and Richardson, P. (2002). Genetic and genomic tools for
Xenopus research: The NIH Xenopus initiative. Null 225,
384–391.
Kroll, K.L., and Amaya, E. (1996). Transgenic Xenopus embryos
from sperm nuclear transplantations reveal FGF signaling
requirements during gastrulation. Development (Cambridge,
England) 122, 3173–3183.
Kwan, K.M., Fujimoto, E., Grabher, C., Mangum, B.D., Hardy,
M.E., Campbell, D.S., Parant, J.M., Yost, H.J., Kanki, J.P.,
and Chien, C. (2007). The Tol2kit: A multisite gatewaybased construction kit for Tol2 transposon transgenesis constructs. Dev Dynam 236, 3088–3099.
Landim-Vieira, M., Johnston, J.R., Ji, W., Mis, E.K., Tijerino, J.,
Spencer-Manzon, M., Jeffries, L., Hall, E.K., PaniselloManterola, D., Khokha, M.K., et al. (2020). Familial dilated
cardiomyopathy associated with a novel combination of compound heterozygous TNNC1 variants. Front Physiol 10 , 1612.
Lane, M.A., Kimber, M., and Khokha, M.K. (2013). Breeding based
remobilization of Tol2 transposon in Xenopus tropicalis.
PLoS One 8, e76807.
Lei, Y., Guo, X., Deng, Y., Chen, Y., and Zhao, H. (2013).
Generation of gene disruptions by transcription activator-like
effector nucleases (TALENs) in Xenopus tropicalis embryos.
Cell & Bioscience 3, 21.
Lei, Y., Guo, X., Liu, Y., Cao, Y., Deng, Y., Chen, X., Cheng, C.H.K.,
Dawid, I.B., Chen, Y., and Zhao, H. (2012). Eff cient targeted gene disruption in Xenopus embryos using engineered
transcription activator-like effector nucleases (TALENs).
Proceedings of the National Academy of Sciences of the
United States of America 109, 17484–17489.
Li, Y.E., Allen, B.G., and Weeks, D.L. (2012). Xenopus protocols, post-genomic approaches. Methods Mol Biology 917,
219–230.
Liu, Z., Cheng, T.T.K., Shi, Z., Liu, Z., Lei, Y., Wang, C., Shi, W.,
Chen, X., Qi, X., Cai, D., et al. (2016). Eff cient genome
editing of genes involved in neural crest development using
the CRISPR/Cas9 system in Xenopus embryos. Cell &
Bioscience 6, 22.
Love, N.R., Chen, Y., Ishibashi, S., Kritsiligkou, P., Lea, R., Koh, Y.,
Gallop, J.L., Dorey, K., and Amaya, E. (2013). Amputationinduced reactive oxygen species are required for successful
Xenopus tadpole tail regeneration. Nature Publishing Group
15, 222–228.
Love, N., Thuret, R., Chen, Y., Ishibashi, S., Sabherwal, N.,
Paredes, R., Alves-Silva, J., Dorey, K., Noble, A.M., Guille,
M.J., et al. (2011a). pTransgenesis: A cross-species, modular
transgenesis resource. Development (Cambridge, England)
138, 5451–5458.
Love, N.R., Thuret, R., Chen, Y., Ishibashi, S., Sabherwal, N.,
Paredes, R., Alves-Silva, J., Dorey, K., Noble, A.M., Guille,
M.J., et al. (2011b). pTransgenesis: A cross-species, modular
transgenesis resource. Development 138, 5451–5458.
Mao, C.-Z., Zheng, L., Zhou, Y.-M., Wu, H.-Y., Xia, J.-B., Liang,
C.-Q., Guo, X.-F., Peng, W.-T., Zhao, H., Cai, W.-B., et al.
(2018). CRISPR/Cas9-mediated effcient and precise targeted
integration of donor DNA harboring double cleavage sites in
Xenopus tropicalis. The FASEB Journal 32, 6495–6509.
Marquez, J., Mann, N., Arana, K., Deniz, E., Ji, W., Konstantino,
M., Mis, E.K., Deshpande, C., Jeffries, L., McGlynn, J., et al.
(2021). DLG5 variants are associated with multiple congenital anomalies including ciliopathy phenotypes. J Med Genet
58, 453–464.
Marsh-Armstrong, N., Cai, L., and Brown, D.D. (2004). Thyroid
hormone controls the development of connections between
the spinal cord and limbs during Xenopus laevis metamorphosis. Proc National Acad Sci 101, 165–170.
Marsh-Armstrong, N., Huang, H., Berry, D.L., and Brown, D.D.
(1999). Germ-line transmission of transgenes in Xenopus
laevis. Proc National Acad Sci 96, 14389–14393.
Miyamoto, K., Suzuki, K.T., Suzuki, M., Sakane, Y., Sakuma, T.,
Herberg, S., Simeone, A., Simpson, D., Jullien, J., Yamamoto,
T., et al. (2015). The expression of TALEN before fertilization provides a rapid knock-out phenotype in Xenopus laevis
founder embryos. PLoS One 10, e0142946.
Mukhi, S., and Brown, D.D. (2011). Transdifferentiation of tadpole
pancreatic acinar cells to duct cells mediated by Notch and
stromelysin-3. Developmental Biology 351, 311–317.
Mukhi, S., Cai, L., and Brown, D.D. (2010). Gene switching at
Xenopus laevis metamorphosis. Developmental Biology 338,
117–126.
Mukhi, S., Horb, M.E., and Brown, D.D. (2009). Remodeling of
insulin producing beta-cells during Xenopus laevis metamorphosis. Developmental Biology 328, 384–391.
Mukhi, S., Mao, J., and Brown, D.D. (2008). Remodeling the
exocrine pancreas at metamorphosis in Xenopus laevis.
Proceedings of the National Academy of Sciences of the
United States of America 105, 8962–8967.
Naert, T., Colpaert, R., Nieuwenhuysen, T.V., Dimitrakopoulou, D.,
Leoen, J., Haustraete, J., Boel, A., Steyaert, W., Lepez, T.,
Deforce, D., et al. (2016). CRISPR/Cas9 mediated knockout of rb1 and rbl1 leads to rapid and penetrant retinoblastoma development in Xenopus tropicalis. Scientif c Reports
6, 35264.
Naert, T., Dimitrakopoulou, D., Tulkens, D., Demuynck, S., Carron,
M., Noelanders, R., Eeckhout, L., Isterdael, G.V., Deforce,
D., Vanhove, C., et al. (2020a). RBL1 (p107) functions as
tumor suppressor in glioblastoma and small-cell pancreatic
neuroendocrine carcinoma in Xenopus tropicalis. Oncogene
39, 2692–2706.
Naert, T., Tulkens, D., Edwards, N.A., Carron, M., Shaidani, N.-I.,
Wlizla, M., Boel, A., Demuynck, S., Horb, M.E., Coucke,
P., et al. (2020b). Maximizing CRISPR/Cas9 phenotype penetrance applying predictive modeling of editing outcomes in
Xenopus and zebrafsh embryos. Sci Rep-Uk 10, 14662.
Nakade, S., Sakuma, T., Sakane, Y., Hara, Y., Kurabayashi, A.,
Kashiwagi, K., Kashiwagi, A., Yamamoto, T., and Obara, M.
(2015). Homeolog-specifc targeted mutagenesis in Xenopus
laevis using TALENs. In Vitro Cellular & Developmental
Biology: Animal 51, 879–884.
Nakade, S., Tsubota, T., Sakane, Y., Kume, S., Sakamoto, N., Obara,
M., Daimon, T., Sezutsu, H., Yamamoto, T., Sakuma, T., et al.
(2014). Microhomology-mediated end-joining-dependent
integration of donor DNA in cells and animals using TALENs
and CRISPR/Cas9. Nature Communications 5, 5560.
Nakai, Y., Nakajima, K., Robert, J., and Yaoita, Y. (2016). Ouro
proteins are not essential to tail regression during Xenopus
tropicalis metamorphosis. Genes Cells 21, 275–286.
Nakajima, K., Nakai, Y., Okada, M., and Yaoita, Y. (2013). Targeted
gene disruption in the Xenopus tropicalis genome using
designed TALE nucleases. Zool Sci 30, 455–460.
Nakajima, K., Nakajima, T., Takase, M., and Yaoita, Y. (2012).
Generation of albino Xenopus tropicalis using zinc-f nger