Analytica Chimica Acta, accepted, 07/07/2015. This is the accepted version without proofing
corrections. DOI: 10.1016/j.aca.2015.06.011
.
Page 21 of 26
Figure S-9: Normal TSFS (top) and HH polarised TSFS (middle) plots for: (left column) N-acetyl-tryptophan
(10
–3 M), (middle column) N-acetyl-tyrosine (10
–3 M), and (right column) a mixture (18:1 molar ratio of Nacetyl-Tyr and N-acetyl-Trp, 10
–3 M total concentration) in 90% glycerol.
Figure S-5: Changes in average anisotropy for HSA (1mg mL
–1 ) for a) thermal denaturation (10–70°C and
cooling to 20ºC), and b) chemical denaturation using GuHCl (0–4 M) at 25°C. The average anisotropy was
calculated by taking the average of all points measured over the λ ex = 290–305nm and Δλ = 40nm (e.g. λ em =
330–345nm) spectral range.
corrections. DOI: 10.1016/j.aca.2015.06.011
.
Page 21 of 26
Figure S-9: Normal TSFS (top) and HH polarised TSFS (middle) plots for: (left column) N-acetyl-tryptophan
(10
–3 M), (middle column) N-acetyl-tyrosine (10
–3 M), and (right column) a mixture (18:1 molar ratio of Nacetyl-Tyr and N-acetyl-Trp, 10
–3 M total concentration) in 90% glycerol.
Figure S-5: Changes in average anisotropy for HSA (1mg mL
–1 ) for a) thermal denaturation (10–70°C and
cooling to 20ºC), and b) chemical denaturation using GuHCl (0–4 M) at 25°C. The average anisotropy was
calculated by taking the average of all points measured over the λ ex = 290–305nm and Δλ = 40nm (e.g. λ em =
330–345nm) spectral range.