Depth (mm)
N 2
November 1981
NH +
4
Total
N 2
July 1982
NH +
4
Total
0-10
6.2
1.1
7.3
18.9
4.6
23.5
10-20
9.8
2.1
11.9
24.4
4,1
28.5
20-30
5.2
6.1
11.3
11.6
3.5
15.1
30-40
2.2
1.3
3.5
4.7
2.4
7.1
40-50
2.3
1.07
3.3
4.4
1.7
6.1
Table 5 : Rates of denitrification and nitrate dissimilation to NH+
4
in Kingoodie Bay
sediments as determined using 15 N-labelled nitrate. Rates are expressed as µg N.d g dry wt.
sediment -1 and are mean values of 3 replicates.
ACKNOWLEDGEMENTS
This work was supported by a research grant (GR/3/4208) to R.A. Herbert from the Natural Environmental
Research Council.
BILLEN G., 1975. Evaluation of nitrifying activity in sediments in dark 14 C-bicarbonate incorporation. Water
Research 10, 51-57.
BILLEN G., 1982. Modelling the processes of organic matter degradation and nutrient recycling in sedimentary
systems. In Sediment Microbiology, Special Publications of the Society for General Microbiology pp. 15-52.
Edited by D.B. Nedwell & C.M. Brown, London, Academic Press.
CAMPBELL N.E.R., and ALEEM M.J.H., 1965. The effect of 2-chloro-6 (trichloromethyl) pyridine on the
chemoautotrophic metabolism on nitrifying bacteria. 1. Ammonia and hydroxylamine oxidation by Nitrosomonas. Antonie van Leeuwenhoek, 31, 124-136.
DUNN G.M., HERBERT, R.A. and BROWN C.M., 1979. Influence of oxygen tension on respiration by a Klebsiella
sp. growing in chemostat culture. Journal of General Microbiology 112, 379-383.
HERBERT R.A., 1982. Nitrate dissimilation in marine and estuarine sediments. In Sediment Microbiology,
Special Publications of the Society for General Microbiology pp. 53-71. Edited by D.B. Nedwell & C.M.
Brown, London, Academic Press.
HERBERT R.A., DUNN G.M. and BROWN, C.M., 1980. The physiology of nitrate dissimilatory bacteria from the
Tay estuary. Proceedings of the Royal Society of Edinburgh 78B, 137-144.
JORGENSEN B. B., 1977. Bacterial sulphate reduction within reduced microniches of oxidised marine sediments.
Marine Biology, 41, 7-17.
KOIKE I. and HATTORI A., 1978. Simultaneous determination of nitrification and nitrate reduction in coastal
sediments by a 15 N-dilution technique. Applied and Environmental Microbiology 35, 853-857.
MACFARLANE G.T., 1983. Physiology of dissimilatory nitrate reduction and nitrification in estuarine sediments. Ph. D. Thesis, University of Dundee, Scotland.
MACFARLANE G.T., and HERBERT R.A., 1982. Nitrate dissimilation by Vibrio spp. isolated from estuarine
sediments. Journal of General Microbiology, 128, 2463-2468.
MACFARLANE G.T., and HERBERT R.A., 1984a. Dissimilatory nitrate reduction and nitrification in estuarine
sediments. Journal of General Microbiology, 130, 2301-2308.
MACFARLANE G.T., and HERBERT R.A., 1984b. Effect of oxygen tension, salinity, temperature and organic
matter concentration on the growth and nitrifying activity of an estuarine strain of Nitrosomonas. FEMS
Microbiology Letters 23, 107-111.
SORENSEN J., 1978. Capacity for denitrification and reduction of NO -
3 to NH4 in a coastal marine sediment.
Applied and Environmental Microbiology, 35, 301-305.
281
N 2
November 1981
NH +
4
Total
N 2
July 1982
NH +
4
Total
0-10
6.2
1.1
7.3
18.9
4.6
23.5
10-20
9.8
2.1
11.9
24.4
4,1
28.5
20-30
5.2
6.1
11.3
11.6
3.5
15.1
30-40
2.2
1.3
3.5
4.7
2.4
7.1
40-50
2.3
1.07
3.3
4.4
1.7
6.1
Table 5 : Rates of denitrification and nitrate dissimilation to NH+
4
in Kingoodie Bay
sediments as determined using 15 N-labelled nitrate. Rates are expressed as µg N.d g dry wt.
sediment -1 and are mean values of 3 replicates.
ACKNOWLEDGEMENTS
This work was supported by a research grant (GR/3/4208) to R.A. Herbert from the Natural Environmental
Research Council.
BILLEN G., 1975. Evaluation of nitrifying activity in sediments in dark 14 C-bicarbonate incorporation. Water
Research 10, 51-57.
BILLEN G., 1982. Modelling the processes of organic matter degradation and nutrient recycling in sedimentary
systems. In Sediment Microbiology, Special Publications of the Society for General Microbiology pp. 15-52.
Edited by D.B. Nedwell & C.M. Brown, London, Academic Press.
CAMPBELL N.E.R., and ALEEM M.J.H., 1965. The effect of 2-chloro-6 (trichloromethyl) pyridine on the
chemoautotrophic metabolism on nitrifying bacteria. 1. Ammonia and hydroxylamine oxidation by Nitrosomonas. Antonie van Leeuwenhoek, 31, 124-136.
DUNN G.M., HERBERT, R.A. and BROWN C.M., 1979. Influence of oxygen tension on respiration by a Klebsiella
sp. growing in chemostat culture. Journal of General Microbiology 112, 379-383.
HERBERT R.A., 1982. Nitrate dissimilation in marine and estuarine sediments. In Sediment Microbiology,
Special Publications of the Society for General Microbiology pp. 53-71. Edited by D.B. Nedwell & C.M.
Brown, London, Academic Press.
HERBERT R.A., DUNN G.M. and BROWN, C.M., 1980. The physiology of nitrate dissimilatory bacteria from the
Tay estuary. Proceedings of the Royal Society of Edinburgh 78B, 137-144.
JORGENSEN B. B., 1977. Bacterial sulphate reduction within reduced microniches of oxidised marine sediments.
Marine Biology, 41, 7-17.
KOIKE I. and HATTORI A., 1978. Simultaneous determination of nitrification and nitrate reduction in coastal
sediments by a 15 N-dilution technique. Applied and Environmental Microbiology 35, 853-857.
MACFARLANE G.T., 1983. Physiology of dissimilatory nitrate reduction and nitrification in estuarine sediments. Ph. D. Thesis, University of Dundee, Scotland.
MACFARLANE G.T., and HERBERT R.A., 1982. Nitrate dissimilation by Vibrio spp. isolated from estuarine
sediments. Journal of General Microbiology, 128, 2463-2468.
MACFARLANE G.T., and HERBERT R.A., 1984a. Dissimilatory nitrate reduction and nitrification in estuarine
sediments. Journal of General Microbiology, 130, 2301-2308.
MACFARLANE G.T., and HERBERT R.A., 1984b. Effect of oxygen tension, salinity, temperature and organic
matter concentration on the growth and nitrifying activity of an estuarine strain of Nitrosomonas. FEMS
Microbiology Letters 23, 107-111.
SORENSEN J., 1978. Capacity for denitrification and reduction of NO -
3 to NH4 in a coastal marine sediment.
Applied and Environmental Microbiology, 35, 301-305.
281