Marine lipids
Purification of marine microalgae lipids
by centrifugal partition chromatography:
application to fatty acids and glycolipids
Gwenaël Baudimant (l) , Christian Renaud (2) , Patrick Durand (2)
(1) Aquaartis, ZI, 29 rue de la Dutée, 44806 Saint-Herblain Cedex, France
info@aquaartis.com
(2) Ifremer Nantes, Drv/vp/bm, BP 21105, 44311 Nantes Cedex 03, France
CPC is a liquid-liquid separation method relating to the partition of
compounds between two immiscible phases of a solvent system, maintained in contact in the chromatography column. One of the liquid
phases, called the stationary phase, is held in the instrument by centrifugal force. The other phase-called the mobile phase, moves through
the column. The separation of compounds is performed according to
the affinity of the molecule for one or the other phase. It is an all-hquid
method which is characterized by the absence of a solid support.
We present in this paper the purification by CPC of fatty acid methyl
esters (FAMEs) and sulfoquinovosyldiacylglycerol (SODG).
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