Biotechnology
Conclusion
This study evidenced that a crude lipid extract can be tised for the formulation of liposomes designed to aquaculture. Thiamine was used as
an example of an hydrosoluble vitamin that could be of interest in the
case of nutritional deficiency. Bilayer impermeability towards this
vitamin, when vesicles are placed in sea water, was increased by liposome surface modification with dextran. Uptake and metabolism of
these liposomes by ovocytes and larvae of Pacific oysters are under
investigation.
Acknowledgements
The authors gratefully acknowledge the valuable assistance of P. Méléard in the
optical microscope observations and granulometry measurements and D. Letourneur
and H. Sahli for their contribution in dextran grafting and characterization.
Bibliographic references
Akiyoshi K., Yamaguchi S., Sunamoto J., 1991- Self-aggregates of
hydrophobic polysaccharide derivatives. Chem. Lett., 1263-1266.
Allen T.M., 1994. The use of glycolipids ancl hydrophilic polymers in
avoiding rapid uptake of liposomes by the mononuclear phagocyte
system. Adv. Drug Delivery Rev., 13, 285-309.
Ames B., 1966. Assay of inorganic phosphate, total phosphate and
phosphatase. Meth. Enzymol., 18, 115-118.
Baudimant G., Maurice M., Landrein A., Durand G., Durand R, 1996.
Purification of phosphatidylcholine with high content of DHA from
squid Illlex argentinus by countercurrent chromatography. J. Liq.
Chromatogr. Relat. Technol., 19, 1793-1804.
Cansell M., Gouygou J.-R, Jozefonvicz J., Letourneur D., 1997. Lipid
composition of cultured endothelial cells in relation to their growth.
Lipids, 32, 39-44.
Cansell M., Parisel C., Jozefonvicz J., Letourneur D. Liposomes coated
with chemically modified dextran interact witli human endothelial
cells. J. Biomed. Mater. Res., (in press).
Kang E.C., Akiyoshi K., Sunamoto J., 1997. Surface coating with
hydrophobized polysaccharides. J. Bioact. Compat. Polym., 31, 14-26.
Hontaria F., Crowe J.H., Crowe L.M., Amat E, 1994. Potential use of
liposomes in larviculture as a delivery system through Artemia
nauplii. Aquaculture, 127, 255-264.
Lasic D.D., 1993. Liposomes: from physics to applications. Elsevier,
Amsterdam, 567 p.
219
Conclusion
This study evidenced that a crude lipid extract can be tised for the formulation of liposomes designed to aquaculture. Thiamine was used as
an example of an hydrosoluble vitamin that could be of interest in the
case of nutritional deficiency. Bilayer impermeability towards this
vitamin, when vesicles are placed in sea water, was increased by liposome surface modification with dextran. Uptake and metabolism of
these liposomes by ovocytes and larvae of Pacific oysters are under
investigation.
Acknowledgements
The authors gratefully acknowledge the valuable assistance of P. Méléard in the
optical microscope observations and granulometry measurements and D. Letourneur
and H. Sahli for their contribution in dextran grafting and characterization.
Bibliographic references
Akiyoshi K., Yamaguchi S., Sunamoto J., 1991- Self-aggregates of
hydrophobic polysaccharide derivatives. Chem. Lett., 1263-1266.
Allen T.M., 1994. The use of glycolipids ancl hydrophilic polymers in
avoiding rapid uptake of liposomes by the mononuclear phagocyte
system. Adv. Drug Delivery Rev., 13, 285-309.
Ames B., 1966. Assay of inorganic phosphate, total phosphate and
phosphatase. Meth. Enzymol., 18, 115-118.
Baudimant G., Maurice M., Landrein A., Durand G., Durand R, 1996.
Purification of phosphatidylcholine with high content of DHA from
squid Illlex argentinus by countercurrent chromatography. J. Liq.
Chromatogr. Relat. Technol., 19, 1793-1804.
Cansell M., Gouygou J.-R, Jozefonvicz J., Letourneur D., 1997. Lipid
composition of cultured endothelial cells in relation to their growth.
Lipids, 32, 39-44.
Cansell M., Parisel C., Jozefonvicz J., Letourneur D. Liposomes coated
with chemically modified dextran interact witli human endothelial
cells. J. Biomed. Mater. Res., (in press).
Kang E.C., Akiyoshi K., Sunamoto J., 1997. Surface coating with
hydrophobized polysaccharides. J. Bioact. Compat. Polym., 31, 14-26.
Hontaria F., Crowe J.H., Crowe L.M., Amat E, 1994. Potential use of
liposomes in larviculture as a delivery system through Artemia
nauplii. Aquaculture, 127, 255-264.
Lasic D.D., 1993. Liposomes: from physics to applications. Elsevier,
Amsterdam, 567 p.
219