Marine lipids
The purification of phosphatidylcholine has been subject of extensive
investigations essentially by classic methods. In this study, we demonstrate the usefulness of CPC for the same purpose and certainly for others
complex lipidic compounds. The production of purified lipidic molecules is currently of great interest either for industrial or analytical
purposes as well. In future work, we will make an attempt to envisage
likely future developments m this field.
Bibliographic references
Baudimant G., Maurice M., Landrein A., Durand G., Durand R, 1996.
Purification of phosphatidylcholine with high content of DHA from
squid Illex argentinus by countercurrent chromatography. J. Liq.
Chromatogr. Relat. Technol., 19, 1793-1804.
Bligh E.G., Dyer W.J., 1959. A rapid method of total extraction and
purification. Can. J. Biochem. Phys., 37, 91 1-917.
Bousquet O., Sellier N., Le Goffic F., 1994. Characterization and purification of polyunsaturated fatty acids from microalgae by gas chromatography-mass spectrometry and countercurrent chromatography.
Chromatographia, 39, 40-44.
Du Q., Shu A., Ito Y., 1996. Purification of fish oil ethyl esters by
high-speed countercurrent chromatography using non-aqueous
solvent system. J. Liq. Chromatogr. Relat. Technol., 19, 1451-1457.
Morrisson W.R., Smith L.M., 1 964. Preparation of fatty methyl esters
and dimethylacetals from lipids with boron fluoride methanol. J.
Lipid Res., 5, 600-608.
Otsuka H., Suzuki A., Yamakawa T., 1983. The application of Droplet countercurrent chromatography (DCC) for the separation of
acidic glycolipids. J. Biochem., 94, 2035-2014.
Robertson A.F., Lands W.E.M., 1962. Cited by Christie W.W. in “Structural analysis of lipids by mean of enzymatic hydrolysis”. In: Lipid
analysis, 2nd edition, 1982, Pergamon Press, Oxford, 155-166.
Rouser G., Kritchevsky G., Yamamoto A„ 1976. Cited by Kates M.
in “General fractionation procedure on a silicic acid column”. In:
Advances in lipid research, Paoletti R. & Kritchevzky G. (eds),
Academic Press, New York, 191-192.
20
The purification of phosphatidylcholine has been subject of extensive
investigations essentially by classic methods. In this study, we demonstrate the usefulness of CPC for the same purpose and certainly for others
complex lipidic compounds. The production of purified lipidic molecules is currently of great interest either for industrial or analytical
purposes as well. In future work, we will make an attempt to envisage
likely future developments m this field.
Bibliographic references
Baudimant G., Maurice M., Landrein A., Durand G., Durand R, 1996.
Purification of phosphatidylcholine with high content of DHA from
squid Illex argentinus by countercurrent chromatography. J. Liq.
Chromatogr. Relat. Technol., 19, 1793-1804.
Bligh E.G., Dyer W.J., 1959. A rapid method of total extraction and
purification. Can. J. Biochem. Phys., 37, 91 1-917.
Bousquet O., Sellier N., Le Goffic F., 1994. Characterization and purification of polyunsaturated fatty acids from microalgae by gas chromatography-mass spectrometry and countercurrent chromatography.
Chromatographia, 39, 40-44.
Du Q., Shu A., Ito Y., 1996. Purification of fish oil ethyl esters by
high-speed countercurrent chromatography using non-aqueous
solvent system. J. Liq. Chromatogr. Relat. Technol., 19, 1451-1457.
Morrisson W.R., Smith L.M., 1 964. Preparation of fatty methyl esters
and dimethylacetals from lipids with boron fluoride methanol. J.
Lipid Res., 5, 600-608.
Otsuka H., Suzuki A., Yamakawa T., 1983. The application of Droplet countercurrent chromatography (DCC) for the separation of
acidic glycolipids. J. Biochem., 94, 2035-2014.
Robertson A.F., Lands W.E.M., 1962. Cited by Christie W.W. in “Structural analysis of lipids by mean of enzymatic hydrolysis”. In: Lipid
analysis, 2nd edition, 1982, Pergamon Press, Oxford, 155-166.
Rouser G., Kritchevsky G., Yamamoto A„ 1976. Cited by Kates M.
in “General fractionation procedure on a silicic acid column”. In:
Advances in lipid research, Paoletti R. & Kritchevzky G. (eds),
Academic Press, New York, 191-192.
20