Function and metabolism
Fatty acid specificity of polar lipid classes
in two bivalves molluscs.
Changes in relation to diet, reproduction
and larval development
J.-F. Samain
(1) , P. Soudant
(1) , Y. Marty ( 1 ) , J. Moal
(1) ,
K. van Ryckeghem
(1) '
,
(1) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés, BP 70,
29280 Plouzané, France-jsamam@ifremer.fr
(2) Université de Bretagne occidentale, UMR-CNRS 6521, BP 809, 29285 Brest Cedex, France
(3) University of Gent, laboratory of Aquaculture & Artemia reference center,
Rozier 44, 9000 Gent, Belgium
The aim of this study is to develop an approach of lipid requirements
for aquacultured marine bivalves during reproduction and larval development. Phospholipids and their PUFA composition play an important
role in all biological membranes. So, specificity in PUFA composition
of polar lipid classes of gonads, eggs and larvae, was studied during the
reproductive cycle in two bivalve species, the scallop Peiten maximm and
the oyster Crassostrea gigas and in different lipid food composition.
The study of the relationships between the lipid food composition and
polar lipid class PUFA composition, was undertaken to characterize
capacities of these bivalve polar lipid classes to balance variations in food
lipid composition. The study during the reproductive phases of the
two bivalves was devoted to identify possible specificities in the role
of these PUFAs and PL classes during this biological cycle.
Scallop and oyster gonads or larvae, fed different algal diets, in the lfremer experimental hatchery or from the wild, were analysed during
conditioning for reproduction and during larval development. Fatty acids
were analysed in the different separated phospholipid classes according
to Soudant et al. (1995, 1999). It has been precedently demonstrated
strong relationships between the lipid composition of the food and
neutral lipids of the gonads or larvae. On the contrary, some similar
selective retention of peculiar diet PUFAs was evidenced in scallop and
oyster phospholipids: all the six separated phospholipid classes in the
two species, demonstrated a stability in the class percentage but had a
specificity in their PUFA composition relatively similar in the two
species. Some identical phospholipid classes resisted to foocl composition changes as others were more influenced by the composition of the
diet. These results demonstrated that compensatory mechanisms exist
in peculiar phospholipid classes as PI, PF, PS, DHA plasmalogens and
a glycolipid, leading to a selective retention of specific essential fatty
acids, suggesting their biological importance for the two species.
187
Fatty acid specificity of polar lipid classes
in two bivalves molluscs.
Changes in relation to diet, reproduction
and larval development
J.-F. Samain
(1) , P. Soudant
(1) , Y. Marty ( 1 ) , J. Moal
(1) ,
K. van Ryckeghem
(1) '
,
(1) Ifremer Brest, Drv/ra, laboratoire de physiologie des invertébrés, BP 70,
29280 Plouzané, France-jsamam@ifremer.fr
(2) Université de Bretagne occidentale, UMR-CNRS 6521, BP 809, 29285 Brest Cedex, France
(3) University of Gent, laboratory of Aquaculture & Artemia reference center,
Rozier 44, 9000 Gent, Belgium
The aim of this study is to develop an approach of lipid requirements
for aquacultured marine bivalves during reproduction and larval development. Phospholipids and their PUFA composition play an important
role in all biological membranes. So, specificity in PUFA composition
of polar lipid classes of gonads, eggs and larvae, was studied during the
reproductive cycle in two bivalve species, the scallop Peiten maximm and
the oyster Crassostrea gigas and in different lipid food composition.
The study of the relationships between the lipid food composition and
polar lipid class PUFA composition, was undertaken to characterize
capacities of these bivalve polar lipid classes to balance variations in food
lipid composition. The study during the reproductive phases of the
two bivalves was devoted to identify possible specificities in the role
of these PUFAs and PL classes during this biological cycle.
Scallop and oyster gonads or larvae, fed different algal diets, in the lfremer experimental hatchery or from the wild, were analysed during
conditioning for reproduction and during larval development. Fatty acids
were analysed in the different separated phospholipid classes according
to Soudant et al. (1995, 1999). It has been precedently demonstrated
strong relationships between the lipid composition of the food and
neutral lipids of the gonads or larvae. On the contrary, some similar
selective retention of peculiar diet PUFAs was evidenced in scallop and
oyster phospholipids: all the six separated phospholipid classes in the
two species, demonstrated a stability in the class percentage but had a
specificity in their PUFA composition relatively similar in the two
species. Some identical phospholipid classes resisted to foocl composition changes as others were more influenced by the composition of the
diet. These results demonstrated that compensatory mechanisms exist
in peculiar phospholipid classes as PI, PF, PS, DHA plasmalogens and
a glycolipid, leading to a selective retention of specific essential fatty
acids, suggesting their biological importance for the two species.
187