SUBJECT INDEX
379
serial sections, for, 212
sources, 360-361
Spherical aberration, 281
Spraying (see
nebulizing)
Stability, electronic, 280, 281, 294-298
Staining, 216-264, 301-303, 329 (Figs. 26,
27)
acetone, penetrant aid, 218
alcohol, penetrant aid, 218
aldehyde fixed tissue, 61-63, 225, 243245, 356 (Fig. 4)
amyl acetate, penetrant aid, 218
Araldite, 217, 224, 355
block, 236, 239, 241
contamination, 214, 221, 225, 227-229,
235, 237, 240, 241 (Fig. 10)
double, 231-234 (Figs. 4, 28 c, d, 40)
Durcupan, 123
Epon, 218, 224
epoxy resins, 83, 216, 217, 224, 242
glycol methacrylate, 217
heat, 218-219, 235, 237, 257, 260
hydroxypropyl methacrylate, 124, 125
ketone vapors, penetrant aid, 218
Maraglas, 217, 224
methacrylate, 82, 97, 121, 216, 217, 224,
231
mixed plastics, 110, 127
negative (see negative staining)
osmium tetroxide fixed tissue, 243-245
polyesters, 216, 217, 218, 224
pretreatments, 2i8
propylene oxide inhibition, 86, 245
secondary, 231-234 (Figs. 4, 28c, d, 40)
Selectron, 218
thick sections, 97, 256-262
trichloroethylene, penetrant aid, 218
Vestopal W, 218, 224, 355
water soluble resins
Stains (electron) (see also particular stains)
alcoholic solutions, 218, 225, 235, 237
alkaline lead hydroxide, 211, 217-234,
243-245 (Figs. 4, 5, 10, 11, 25, 27,
39, 40)
antibody, 247-250
Bullivant and Hotchin chromyl
chloride, 242-243
Callahan and Horner vanadium salts,
241-242
chromate, 41
chromyl chloride, 242-243
chromyl chloride, frozen-dried tissue, 75
conjugated antibody, 247-250
Dalton and Zeigel's technique, 230-231
Karnovsky's lead hydroxide, 219, 230
Lawn's permanganate, 239-241
lead hydroxide, 82
lead subacetate, 231
Lever's lead hydroxide, 219, 230
Millonig's lead tartrate, 219, 229-230
osmium tetroxide, 82, 216
Parson's permanganate, 241
penetration, 217-219, 237
permanganate, 239-241
phosphotungstic
acid, 216, 218,
234,
236-239, 244 (Figs. 6, 7, 24, 29, 38)
Reynold's lead citrate, 219, 221, 223-225,
229, 355 (Fig. 26)
silver impregnation, 244-245
uranyl acetate, 78, 217, 218,
234-236
(Figs. 4, 28b, c, d, 40)
uranyl nitrate, 236, 348
vanadatomolybdate, 242
vanadyl sulfate, 241-242
Watson's lead hydroxide, 82, 217, 219,
221, 223, 225-229, 236 (Figs. 3, 27,
28a, c, d)
Stigmator, 3-4, 267-268, 291-293
Styrene, transitional solvent, 87, 88, 118,
355
Sudan dyes, 260-261
Sulphur-35 radioisotope, 251
Supporting films, 187, 192-213 (Figs. 22,
23, 24, 28d, 38)
adhesive for, 208
carbon, 192-193, 200-209, 211, 328 (Fig.
23)
carbon stabilized, 192, 199, 200, 205,
206-207, 212, 294, 309, 328 (Fig. 23)
dry stripping, 330
Formvar, 192, 198-199, 201, 206, 207,
211, 212, 213, 328
historical, 192
methacrylate, 201, 211-212
379
serial sections, for, 212
sources, 360-361
Spherical aberration, 281
Spraying (see
nebulizing)
Stability, electronic, 280, 281, 294-298
Staining, 216-264, 301-303, 329 (Figs. 26,
27)
acetone, penetrant aid, 218
alcohol, penetrant aid, 218
aldehyde fixed tissue, 61-63, 225, 243245, 356 (Fig. 4)
amyl acetate, penetrant aid, 218
Araldite, 217, 224, 355
block, 236, 239, 241
contamination, 214, 221, 225, 227-229,
235, 237, 240, 241 (Fig. 10)
double, 231-234 (Figs. 4, 28 c, d, 40)
Durcupan, 123
Epon, 218, 224
epoxy resins, 83, 216, 217, 224, 242
glycol methacrylate, 217
heat, 218-219, 235, 237, 257, 260
hydroxypropyl methacrylate, 124, 125
ketone vapors, penetrant aid, 218
Maraglas, 217, 224
methacrylate, 82, 97, 121, 216, 217, 224,
231
mixed plastics, 110, 127
negative (see negative staining)
osmium tetroxide fixed tissue, 243-245
polyesters, 216, 217, 218, 224
pretreatments, 2i8
propylene oxide inhibition, 86, 245
secondary, 231-234 (Figs. 4, 28c, d, 40)
Selectron, 218
thick sections, 97, 256-262
trichloroethylene, penetrant aid, 218
Vestopal W, 218, 224, 355
water soluble resins
Stains (electron) (see also particular stains)
alcoholic solutions, 218, 225, 235, 237
alkaline lead hydroxide, 211, 217-234,
243-245 (Figs. 4, 5, 10, 11, 25, 27,
39, 40)
antibody, 247-250
Bullivant and Hotchin chromyl
chloride, 242-243
Callahan and Horner vanadium salts,
241-242
chromate, 41
chromyl chloride, 242-243
chromyl chloride, frozen-dried tissue, 75
conjugated antibody, 247-250
Dalton and Zeigel's technique, 230-231
Karnovsky's lead hydroxide, 219, 230
Lawn's permanganate, 239-241
lead hydroxide, 82
lead subacetate, 231
Lever's lead hydroxide, 219, 230
Millonig's lead tartrate, 219, 229-230
osmium tetroxide, 82, 216
Parson's permanganate, 241
penetration, 217-219, 237
permanganate, 239-241
phosphotungstic
acid, 216, 218,
234,
236-239, 244 (Figs. 6, 7, 24, 29, 38)
Reynold's lead citrate, 219, 221, 223-225,
229, 355 (Fig. 26)
silver impregnation, 244-245
uranyl acetate, 78, 217, 218,
234-236
(Figs. 4, 28b, c, d, 40)
uranyl nitrate, 236, 348
vanadatomolybdate, 242
vanadyl sulfate, 241-242
Watson's lead hydroxide, 82, 217, 219,
221, 223, 225-229, 236 (Figs. 3, 27,
28a, c, d)
Stigmator, 3-4, 267-268, 291-293
Styrene, transitional solvent, 87, 88, 118,
355
Sudan dyes, 260-261
Sulphur-35 radioisotope, 251
Supporting films, 187, 192-213 (Figs. 22,
23, 24, 28d, 38)
adhesive for, 208
carbon, 192-193, 200-209, 211, 328 (Fig.
23)
carbon stabilized, 192, 199, 200, 205,
206-207, 212, 294, 309, 328 (Fig. 23)
dry stripping, 330
Formvar, 192, 198-199, 201, 206, 207,
211, 212, 213, 328
historical, 192
methacrylate, 201, 211-212