378
SUBJECT INDEX
S
Sabatini, Bensch and Barrnett's buffered
aldehydes, 52, 53, 57-59, 62
Saliva, wetting agent, 163, 328
Salt solutions
contraindications, 19, 22, 23, 24, 28
perfusion, 24, 26, 27
"Sandwiching" (see section mounting)
Schiff reaction, 245, 259, 261
Schneider's acetocarmine, 261
Scratches, 182, 183
Section mounting, 192-215, 355
methacrylate, 192, 209-212
sandwiching, 95, 105, 209-212 (Figs. 9
(legend), 24, 28d, 38)
serial, 193, 212-213
Section orientation, 169
Section size, 95, 105, 130, 355
Section thickness, 181, 182, 184-186, 299,
301-303, 309, 355 (Fig. 35, 36)
Section transfer, 187-189 (Fig. 18)
Sectioning, 136-191
Araldite, 110, 123, 178 (Fig. 3, 4, 5, 29,
39)
block orientation, 173-174
clearance angle, 171-173, 177, 181, 182,
186, 355
compression, 162, 174
connective tissue, 163, 173
cross-linked methacrylate, 93, 121 (Fig.
28)
difficulties, 181-184, 186-187
Epon, 113-115, 163, 176 (Figs. 10, 11, 40)
epoxy plastics, 93, 174, 175-176
flattening
sections, 175-176
flotation,
93, 136, 174-176, 355
free-hand, 131
frozen-dried tissue, 74-75
frozen-substituted tissue, 78
glycol methacrylate, 124, 174
historical, 136-137
hydroxypropyl
methacrylate,
124-125,
163
knife tilt, 171-173, 177, 181, 182, 186,
355
methacrylate, 93, 97, 174, 175, 178 (Figs.
2, 7, 8, 9, 24, 30, 35, 36, 37, 38)
polyester plastics, 174, 175-176
serial, 142, 193, 212-213
speed, 126, 138, 140-142, 178, 182, 186.
310
Vestopal W, 118, 178 (Fig. 12)
Sections, thick (see thick sections)
Selectron
embedding, 119-120, 127
staining, 218
Selectron 5003, 119, 127, 128
Selectron 5208, 119, 127
Selectron 5214,
119, 127
Service personnel, 8-9, 269, 280, 295, 298
Shadowing, 265, 323, 326, 327, 330, 331-334
(Figs. 9, 41)
angle, 334
contamination, 327-328
equipment, 6, 332
film thickness, 204-205, 333-334
materials, 332-333
quantitative
measurements,
330,
332,
334
replicas, 326, 335, 336, 341
tungsten baskets, 332
Shutter speed, 4, 308, 309
Silica replicas, 327, 342
Silver impregnation, 244-245
Silver nitrate negative stain, 348
SI-RO-FLEX ultramicrotome, 145-146
Sitte ultramicrotome, 143-144
Sjostrand microtome, 137
Sodium tungstate negative stain, 348
Sodium methoxide solvent, 257, 259, 262263
Sorvall MT-2 ultramicrotome, 131, 146147
Source alignment (see also filament, anode,
and condenser lens alignment)
284-287, 288 (Fig. 33)
Space charge effects, 269
Specimen grids, 187, 189-190, 212,
334
adhesive for, 208
cleaning, 190, 215
etching, 215
gold, 334
mounting, 196-197, 206, 207, 208 (Fig.
22)
SUBJECT INDEX
S
Sabatini, Bensch and Barrnett's buffered
aldehydes, 52, 53, 57-59, 62
Saliva, wetting agent, 163, 328
Salt solutions
contraindications, 19, 22, 23, 24, 28
perfusion, 24, 26, 27
"Sandwiching" (see section mounting)
Schiff reaction, 245, 259, 261
Schneider's acetocarmine, 261
Scratches, 182, 183
Section mounting, 192-215, 355
methacrylate, 192, 209-212
sandwiching, 95, 105, 209-212 (Figs. 9
(legend), 24, 28d, 38)
serial, 193, 212-213
Section orientation, 169
Section size, 95, 105, 130, 355
Section thickness, 181, 182, 184-186, 299,
301-303, 309, 355 (Fig. 35, 36)
Section transfer, 187-189 (Fig. 18)
Sectioning, 136-191
Araldite, 110, 123, 178 (Fig. 3, 4, 5, 29,
39)
block orientation, 173-174
clearance angle, 171-173, 177, 181, 182,
186, 355
compression, 162, 174
connective tissue, 163, 173
cross-linked methacrylate, 93, 121 (Fig.
28)
difficulties, 181-184, 186-187
Epon, 113-115, 163, 176 (Figs. 10, 11, 40)
epoxy plastics, 93, 174, 175-176
flattening
sections, 175-176
flotation,
93, 136, 174-176, 355
free-hand, 131
frozen-dried tissue, 74-75
frozen-substituted tissue, 78
glycol methacrylate, 124, 174
historical, 136-137
hydroxypropyl
methacrylate,
124-125,
163
knife tilt, 171-173, 177, 181, 182, 186,
355
methacrylate, 93, 97, 174, 175, 178 (Figs.
2, 7, 8, 9, 24, 30, 35, 36, 37, 38)
polyester plastics, 174, 175-176
serial, 142, 193, 212-213
speed, 126, 138, 140-142, 178, 182, 186.
310
Vestopal W, 118, 178 (Fig. 12)
Sections, thick (see thick sections)
Selectron
embedding, 119-120, 127
staining, 218
Selectron 5003, 119, 127, 128
Selectron 5208, 119, 127
Selectron 5214,
119, 127
Service personnel, 8-9, 269, 280, 295, 298
Shadowing, 265, 323, 326, 327, 330, 331-334
(Figs. 9, 41)
angle, 334
contamination, 327-328
equipment, 6, 332
film thickness, 204-205, 333-334
materials, 332-333
quantitative
measurements,
330,
332,
334
replicas, 326, 335, 336, 341
tungsten baskets, 332
Shutter speed, 4, 308, 309
Silica replicas, 327, 342
Silver impregnation, 244-245
Silver nitrate negative stain, 348
SI-RO-FLEX ultramicrotome, 145-146
Sitte ultramicrotome, 143-144
Sjostrand microtome, 137
Sodium tungstate negative stain, 348
Sodium methoxide solvent, 257, 259, 262263
Sorvall MT-2 ultramicrotome, 131, 146147
Source alignment (see also filament, anode,
and condenser lens alignment)
284-287, 288 (Fig. 33)
Space charge effects, 269
Specimen grids, 187, 189-190, 212,
334
adhesive for, 208
cleaning, 190, 215
etching, 215
gold, 334
mounting, 196-197, 206, 207, 208 (Fig.
22)