380
SUBJECT INDEX
nets, 199-200, 292, 352
Parlodion, 192, 192-198, 199, 201, 206,
207, 211, 212, 328 (Fig. 22)
"sandwiches", 95, 105, 209-212 (Figs. 9
(legend), 24, 28d, 38)
stability, 200, 201, 205, 206, 208-209
stripping, 193-196, 198-199, 208-209 (Fig.
22)
thickness, 192, 197, 199, 200, 204-205,
207, 211-212, 309
wetting, 328, 349-351
Suspensions (see particulate specimens)
T
Tahmisian's isotonic osmium tetroxide, 39
Tahmisian's isotonic permanganate
fixatives, 65
Tahmisian's
permanganate-osmium
tetroxide fixative, 44, 67
Technical help, 10
Tetramethylenedimethacrylate
monomer,
126
Thallium carbonate stain, 347
Thallium fluoride stain, 348
Thick sections
alcoholic stains, 258
alkaline stains, 257-258
bleaching osmium tetroxide, 258
borax stains, 258, 260
dark field examination, 256-257
epoxy, 257-259
examination, 131
freehand, 256
heat effect on staining, 257
negative stain, 262
pH of stain, 257-258
phase contrast microscopy, 131, 256-257
polyester, 257-259
transferring to slides, 256-257
Thionine, 260
Tissue cultures, 31, 39, 42, 49, 101, 104
Tissue exposure, 14-33
Toluene, transitional solvent, 87
Toluidine blue, 259-260, 261
Training program, 354-356
Transfer of tissues, 85-86
Transitional solvents, 85, 86-87, 109
acetone, 87, 118
propylene oxide, 86, 87, 88, 109, 117.
118, 354
styrene, 87, 88, 118, 355
toluene, 87
xylene, 87
Trichloroethylene solvent, 176, 218, 257,
262
Tridimethylaminomethylphenol,
accelerator (see DMP-30)
Trimming blocks, 92-95, 129-134, 173-174,
182 (Fig. 13)
Trimming tissue, 16-23, 25, 29-30, 84
Tritium radioisotope, 251
Troughs
construction, 165-169 (Figs. 18, 19)
contamination, 165, 167, 169, 177, 182184, 187
fluid, 93, 126, 163, 165, 174-175, 182-183,
186
historical, 136, 174
meniscus, 93, 162, 174-175, 177, 185, 187
size, 165
U
"Ultratome", LKB, 131
Ultraviolet polymerization, 77, 98-99, 124
Uranium for shadowing, 333
Uranyl acetate
fixative,
43, 236
negative stain, 348
stain, 78, 217, 218, 234-236 (Figs. 4, 28b,
c, d, 40)
Uranyl nitrate
negative stain, 348
polymerization damage inhibitor, 105
stain, 236
V
Vacuum evaporation, carbon, 3, 6, 192,
200-205, 327, 335-336, 339, 343-344
(Figs. 23, 41)
Vanadatomolybdate stain, 242
Vanadyl sulfate stain, 241-242
SUBJECT INDEX
nets, 199-200, 292, 352
Parlodion, 192, 192-198, 199, 201, 206,
207, 211, 212, 328 (Fig. 22)
"sandwiches", 95, 105, 209-212 (Figs. 9
(legend), 24, 28d, 38)
stability, 200, 201, 205, 206, 208-209
stripping, 193-196, 198-199, 208-209 (Fig.
22)
thickness, 192, 197, 199, 200, 204-205,
207, 211-212, 309
wetting, 328, 349-351
Suspensions (see particulate specimens)
T
Tahmisian's isotonic osmium tetroxide, 39
Tahmisian's isotonic permanganate
fixatives, 65
Tahmisian's
permanganate-osmium
tetroxide fixative, 44, 67
Technical help, 10
Tetramethylenedimethacrylate
monomer,
126
Thallium carbonate stain, 347
Thallium fluoride stain, 348
Thick sections
alcoholic stains, 258
alkaline stains, 257-258
bleaching osmium tetroxide, 258
borax stains, 258, 260
dark field examination, 256-257
epoxy, 257-259
examination, 131
freehand, 256
heat effect on staining, 257
negative stain, 262
pH of stain, 257-258
phase contrast microscopy, 131, 256-257
polyester, 257-259
transferring to slides, 256-257
Thionine, 260
Tissue cultures, 31, 39, 42, 49, 101, 104
Tissue exposure, 14-33
Toluene, transitional solvent, 87
Toluidine blue, 259-260, 261
Training program, 354-356
Transfer of tissues, 85-86
Transitional solvents, 85, 86-87, 109
acetone, 87, 118
propylene oxide, 86, 87, 88, 109, 117.
118, 354
styrene, 87, 88, 118, 355
toluene, 87
xylene, 87
Trichloroethylene solvent, 176, 218, 257,
262
Tridimethylaminomethylphenol,
accelerator (see DMP-30)
Trimming blocks, 92-95, 129-134, 173-174,
182 (Fig. 13)
Trimming tissue, 16-23, 25, 29-30, 84
Tritium radioisotope, 251
Troughs
construction, 165-169 (Figs. 18, 19)
contamination, 165, 167, 169, 177, 182184, 187
fluid, 93, 126, 163, 165, 174-175, 182-183,
186
historical, 136, 174
meniscus, 93, 162, 174-175, 177, 185, 187
size, 165
U
"Ultratome", LKB, 131
Ultraviolet polymerization, 77, 98-99, 124
Uranium for shadowing, 333
Uranyl acetate
fixative,
43, 236
negative stain, 348
stain, 78, 217, 218, 234-236 (Figs. 4, 28b,
c, d, 40)
Uranyl nitrate
negative stain, 348
polymerization damage inhibitor, 105
stain, 236
V
Vacuum evaporation, carbon, 3, 6, 192,
200-205, 327, 335-336, 339, 343-344
(Figs. 23, 41)
Vanadatomolybdate stain, 242
Vanadyl sulfate stain, 241-242