376
SUBJECT INDEX
P
Palade's Veronal buffered tetroxide, 3537, 39
Paladium for shadowing, 333
Paraformaldehyde
fixative,
52-53, 78
Parlodion (see also supporting
films)
embedding, 82
replicas, 340, 342-344 (Fig. 41)
solvents for, 193, 197, 198, 207
Parson's permanganate stain, 241
Particulate specimens
aerosols, 329-331, 352
antibody stains, 249
fragmentation, 328
microscopy, 265
mounting, 192, 329-331
nebulizing, 329-331
negative staining, 346-349
quantitative measurements, 330
shadowing, 326, 331-334
suspension, 328
Pease's phosphate buffered formalin, 5152, 354
Pellets, 43, 103
Peracetic acid reagent, 258-259
Perfusion of
fixatives
(see also Fixation
and particular
fixatives),
14-15, 23-28,
35, 55-56 (Figs. 3, 12, 39, 40)
Periodic acid-Schiff reaction, 259, 261
Permanganate
fixatives,
19, 35, 63-67, 107
duration, 65
Luffs, 63-65 (Fig. 5)
methacrylate
embedding
contraindicated, 66
Tahmisian's isotonic
fixatives,
65
Tahmisian's permanganate-osmium tetroxide, 44, 67
Permanganate stain (see
stain, permanganate)
Permanganate, thick section stain, 260
Phase contrast microscopy, 95, 131, 256257
Phenylenediamine stain, 261
Phloxine B, 259
Phosphate buffer, 39-40, 50-52, 55
Phosphotungstic acid, negative stain
(see
sodium phosphotungstate)
Phosphotungstic acid stain, 216, 218, 234,
236-239, 244 (Figs. 6, 7, 24, 29, 38)
Photography (see also focus and contrast),
299-325
Photographic developers (see
developers)
Photographic plates (see plates)
Photographic printing (see enlarging)
Photometer, 5, 319
Picrofuchsin, 261
Plates, photographic
contrast, 310-311 (Fig. 37)
development, 313-314
intensification,
321
reduction, 320-321
type, 299, 310-313, 314
Platinic chloride stain, 78
Platinum for shadowing, 333
Platinum and carbon for shadowing, 333
Polyampholyte embedding, 125-126, 247,
250
Polyesters (see also particular resins)
embedding, 83, 87, 88-95, 107, 117-120
sectioning, 92-95, 174, 175-176
staining, 216, 217, 218, 224
styrene mixtures, 128
thick-section staining, 258-259
Polymerization (see individual resins)
damage, 49, 104-105, 107, 122 (Fig. 8)
Polystyrene
embedding,
127-128
latex particles, 304, 330, 361
replicas, 341
Polyvinyl alcohol replicas, 341
Porter-Blum microtome, 89, 137-141, 146,
178
Post-mortem change, 15-16
Prepolymerized
methacrylate,
103-104,
122, 124, 125 (Fig. 8)
Printing, photographic (see enlarging)
Product X 133/2097 (see Durcupan)
Projector aperture, 268-269
alignment, 268
contamination,
296-297
Projector lens
alignment, 282, 283
contamination,
296-297
Propane, freezing bath, 68
SUBJECT INDEX
P
Palade's Veronal buffered tetroxide, 3537, 39
Paladium for shadowing, 333
Paraformaldehyde
fixative,
52-53, 78
Parlodion (see also supporting
films)
embedding, 82
replicas, 340, 342-344 (Fig. 41)
solvents for, 193, 197, 198, 207
Parson's permanganate stain, 241
Particulate specimens
aerosols, 329-331, 352
antibody stains, 249
fragmentation, 328
microscopy, 265
mounting, 192, 329-331
nebulizing, 329-331
negative staining, 346-349
quantitative measurements, 330
shadowing, 326, 331-334
suspension, 328
Pease's phosphate buffered formalin, 5152, 354
Pellets, 43, 103
Peracetic acid reagent, 258-259
Perfusion of
fixatives
(see also Fixation
and particular
fixatives),
14-15, 23-28,
35, 55-56 (Figs. 3, 12, 39, 40)
Periodic acid-Schiff reaction, 259, 261
Permanganate
fixatives,
19, 35, 63-67, 107
duration, 65
Luffs, 63-65 (Fig. 5)
methacrylate
embedding
contraindicated, 66
Tahmisian's isotonic
fixatives,
65
Tahmisian's permanganate-osmium tetroxide, 44, 67
Permanganate stain (see
stain, permanganate)
Permanganate, thick section stain, 260
Phase contrast microscopy, 95, 131, 256257
Phenylenediamine stain, 261
Phloxine B, 259
Phosphate buffer, 39-40, 50-52, 55
Phosphotungstic acid, negative stain
(see
sodium phosphotungstate)
Phosphotungstic acid stain, 216, 218, 234,
236-239, 244 (Figs. 6, 7, 24, 29, 38)
Photography (see also focus and contrast),
299-325
Photographic developers (see
developers)
Photographic plates (see plates)
Photographic printing (see enlarging)
Photometer, 5, 319
Picrofuchsin, 261
Plates, photographic
contrast, 310-311 (Fig. 37)
development, 313-314
intensification,
321
reduction, 320-321
type, 299, 310-313, 314
Platinic chloride stain, 78
Platinum for shadowing, 333
Platinum and carbon for shadowing, 333
Polyampholyte embedding, 125-126, 247,
250
Polyesters (see also particular resins)
embedding, 83, 87, 88-95, 107, 117-120
sectioning, 92-95, 174, 175-176
staining, 216, 217, 218, 224
styrene mixtures, 128
thick-section staining, 258-259
Polymerization (see individual resins)
damage, 49, 104-105, 107, 122 (Fig. 8)
Polystyrene
embedding,
127-128
latex particles, 304, 330, 361
replicas, 341
Polyvinyl alcohol replicas, 341
Porter-Blum microtome, 89, 137-141, 146,
178
Post-mortem change, 15-16
Prepolymerized
methacrylate,
103-104,
122, 124, 125 (Fig. 8)
Printing, photographic (see enlarging)
Product X 133/2097 (see Durcupan)
Projector aperture, 268-269
alignment, 268
contamination,
296-297
Projector lens
alignment, 282, 283
contamination,
296-297
Propane, freezing bath, 68