374
SUBJECT INDEX
sectioning, 117
staining, 117, 217, 224
Maraglas 665, 115
Materials, sources, 360-361
Mayer's acid hemalum, 261
Metal evaporation (see shadowing)
Metallurgical techniques, 162, 265, 326,
340, 341
Methacrolein, 57
Methacrylate
autoradiographic embedment, 252
beam damage, 82-83, 95, 105-107, 120121
carbon stabilized sections, 205-206
cross-linked, 83, 93, 120-122, 124-125
decomposition, 82-83, 95, 105-107, 120121 (Figs. 9, 24)
embedding, 82-83, 87, 93, 95-107, 124
explosion damage, 50
frozen-dried tissue impregnation, 73
heat damage, 82, 83, 105, 205-206, 209,
211 (Fig. 9)
heat polymerization, 98-100, 103, 124
hydroxyethyl methacrylate mixture, 124
immunological reactions, 249
inhibitor removal, 98
polymerization damage, 31, 49, 100-105,
107 (Fig. 8)
prepolymerization,
103-104,
122,
124,
125 (Fig. 8b)
replicas, 341
"sandwiched" sections, 95, 105, 209-212
(Figs. 9 (legend), 24, 28d, 38)
sectioning, 93, 95, 97, 121, 125, 174, 175,
178
sections (Figs. 2, 7, 8, 9, 24, 30, 35, 36,
37, 38)
Selectron mixtures, 119, 127
staining, 82, 97, 121, 216, 217, 224, 231
styrene mixtures, 127-128
thick sections, 97, 257, 259-260
ultraviolet polymerization, 98-100, 103,
124
volatilization, 82-83, 95, 105-107, 120-121
(Fig. 9)
Methacrylic acid monomer, 126
Methoxide solvent (see sodium methoxide)
Methyl alcohol dehydration, 85
Methyl cellosolve solvent, 76
Methyl cellulose replicas, 341
Methylmethacrylate, 97, 103, 121
Methyl nadic anhydride, hardener, 111
Mica, film substrate, 208
Michaelis buffer, 36
Microdroplets (see
nebulizing)
Micromanipulation of serial sections, 213
Microscopy, 265-298
Microtomes
Cambridge, 144-145
Clevenger, 147-148
Farrant and Powell, 145-146
Fernandez-Moran, 143
historical, 136-138
Huxley, 144-145
JUM-5, 146
Leitz, 143
LKB "Ultratome," 131, 140-142
Porter-Blum,
89,
137-141,
146,
178
(Fig. 20d)
Reichert, 143-144
SI-RO-FLEX, 145-146
Sitte, 143-144
Sjostrand, 137
Sorvall MT-2, 131, 146-147
Millonig's lead tartrate stain, 219, 229-230
Millonig's
phosphate
buffered
osmium
tetroxide fixation, 39-40, 354
Mincing tissue, 16-20, 29, 45, 354 (Fig. 1)
Mitochondria,
15, 47, 61-62,
66,
101,
(Figs. 2, 3, 4, 6, 28)
Molecular sieves, 85
Movement, 289, 294, 297, 307, 309
Mucin
silver impregnation, 245
Mucopolysaccharides
PTA stain, 236 (Fig. 29)
Silver impregnation, 245
N
Nebulizing, 329-331, 349, 352
Negative staining, 326, 327, 328, 346-353
(Fig. 42)
history, 346
SUBJECT INDEX
sectioning, 117
staining, 117, 217, 224
Maraglas 665, 115
Materials, sources, 360-361
Mayer's acid hemalum, 261
Metal evaporation (see shadowing)
Metallurgical techniques, 162, 265, 326,
340, 341
Methacrolein, 57
Methacrylate
autoradiographic embedment, 252
beam damage, 82-83, 95, 105-107, 120121
carbon stabilized sections, 205-206
cross-linked, 83, 93, 120-122, 124-125
decomposition, 82-83, 95, 105-107, 120121 (Figs. 9, 24)
embedding, 82-83, 87, 93, 95-107, 124
explosion damage, 50
frozen-dried tissue impregnation, 73
heat damage, 82, 83, 105, 205-206, 209,
211 (Fig. 9)
heat polymerization, 98-100, 103, 124
hydroxyethyl methacrylate mixture, 124
immunological reactions, 249
inhibitor removal, 98
polymerization damage, 31, 49, 100-105,
107 (Fig. 8)
prepolymerization,
103-104,
122,
124,
125 (Fig. 8b)
replicas, 341
"sandwiched" sections, 95, 105, 209-212
(Figs. 9 (legend), 24, 28d, 38)
sectioning, 93, 95, 97, 121, 125, 174, 175,
178
sections (Figs. 2, 7, 8, 9, 24, 30, 35, 36,
37, 38)
Selectron mixtures, 119, 127
staining, 82, 97, 121, 216, 217, 224, 231
styrene mixtures, 127-128
thick sections, 97, 257, 259-260
ultraviolet polymerization, 98-100, 103,
124
volatilization, 82-83, 95, 105-107, 120-121
(Fig. 9)
Methacrylic acid monomer, 126
Methoxide solvent (see sodium methoxide)
Methyl alcohol dehydration, 85
Methyl cellosolve solvent, 76
Methyl cellulose replicas, 341
Methylmethacrylate, 97, 103, 121
Methyl nadic anhydride, hardener, 111
Mica, film substrate, 208
Michaelis buffer, 36
Microdroplets (see
nebulizing)
Micromanipulation of serial sections, 213
Microscopy, 265-298
Microtomes
Cambridge, 144-145
Clevenger, 147-148
Farrant and Powell, 145-146
Fernandez-Moran, 143
historical, 136-138
Huxley, 144-145
JUM-5, 146
Leitz, 143
LKB "Ultratome," 131, 140-142
Porter-Blum,
89,
137-141,
146,
178
(Fig. 20d)
Reichert, 143-144
SI-RO-FLEX, 145-146
Sitte, 143-144
Sjostrand, 137
Sorvall MT-2, 131, 146-147
Millonig's lead tartrate stain, 219, 229-230
Millonig's
phosphate
buffered
osmium
tetroxide fixation, 39-40, 354
Mincing tissue, 16-20, 29, 45, 354 (Fig. 1)
Mitochondria,
15, 47, 61-62,
66,
101,
(Figs. 2, 3, 4, 6, 28)
Molecular sieves, 85
Movement, 289, 294, 297, 307, 309
Mucin
silver impregnation, 245
Mucopolysaccharides
PTA stain, 236 (Fig. 29)
Silver impregnation, 245
N
Nebulizing, 329-331, 349, 352
Negative staining, 326, 327, 328, 346-353
(Fig. 42)
history, 346