SUBJECT INDEX
373
H
Hansen's iron-trioxyhematein, 261
Hardener 964B,
108
Heat
damage (see beam damage)
effect on staining, 218-219, 235, 237, 257,
260
Heidenhain's iron-hematoxylin, 261
Helander's alkaline osmium tetroxide, 44
Hemalum, 261
Hematoxylin, 258-259
Hexahydrophthalic anhydride, hardener,
113
Histochemical techniques, 35, 36, 50-51,
56-59, 75, 83, 86, 107, 122, 124, 245247, 329
Holt and Hicks' phosphate buffered formalin, 50, 53
Human tissue, 29-31, 51
Huxley ultramicrotome, 144-145
Hyaluronidase, 28-29, 30
Hydroxyadipaldehyde, 51, 57, 59
Hydroxyethyl
methacrylate
(see
glycol
methacrylate)
Hydroxypropyl methacrylate, 123-125
freeze-substitution, 76
histochemical reactions, 246
prepolymerization, 124
staining, 124, 125
sectioning, 124-125, 163
I
Illumination
(see
beam
intensity
and
source)
Image movements, 289, 294, 297, 307, 309
Impregnation (see
infiltration)
Infiltration, 86-92, 94, 95, 109, 117, 124,
355
duration, 87, 355
frozen-dried specimens, 73-74
frozen-substituted specimens, 75-80
Intensity, beam (see beam intensity)
Interference colors, 181, 182, 184-186, 355
Intermediate lens
alignment, 282, 283, 284
Intermediate
solvents
(see
transitional
solvents)
Interpretation
of
micrographs,
321-325
(Figs. 39, 40)
Isobutyric acid solvent, 76
Isopentane freezing bath, 68
j
JUM-5 ultramicrotome, 146
K
Karnovsky's lead hydroxide, 219, 230
Kellenberger, Ryter and Sechaud bacterial
fixation,
42-43
Ketone solvents, 257, 262
Knives for sectioning
(see
glass knives,
diamond knives)
L
Labels
capsules, 89
Laboratory
equipment, 2-6
layout, 1-3
management, 6-8
Lanthanum acetate stain, 78
Lawn's permanganate stain (see
stain,
Lawn's permanganate)
Lead stains (see stains, alkaline lead)
Lead subacetate stain, 231
Leitz ultramicrotome, 143
Lenses (see
particular lenses,
condenser,
objective, intermediate, or projector)
Lever's lead hydroxide, 219, 230
Light green, 261
LKB knife making apparatus, 158
LKB "Ultratome," 131, 140-142
Luft's permanganate fixative, 63-65 (Fig. 5)
Lugol's solution, 261
Luperco CDB catalyst, 97-98, 124
M
Macromolecules, 326, 336, 337, 343, 352
Magnetic fields, 1-2
Magnification, 214, 301-303 (Figs. 35, 36)
Mallory's azure II-methylene blue, 260
Maraglas
embedding, 115-117
373
H
Hansen's iron-trioxyhematein, 261
Hardener 964B,
108
Heat
damage (see beam damage)
effect on staining, 218-219, 235, 237, 257,
260
Heidenhain's iron-hematoxylin, 261
Helander's alkaline osmium tetroxide, 44
Hemalum, 261
Hematoxylin, 258-259
Hexahydrophthalic anhydride, hardener,
113
Histochemical techniques, 35, 36, 50-51,
56-59, 75, 83, 86, 107, 122, 124, 245247, 329
Holt and Hicks' phosphate buffered formalin, 50, 53
Human tissue, 29-31, 51
Huxley ultramicrotome, 144-145
Hyaluronidase, 28-29, 30
Hydroxyadipaldehyde, 51, 57, 59
Hydroxyethyl
methacrylate
(see
glycol
methacrylate)
Hydroxypropyl methacrylate, 123-125
freeze-substitution, 76
histochemical reactions, 246
prepolymerization, 124
staining, 124, 125
sectioning, 124-125, 163
I
Illumination
(see
beam
intensity
and
source)
Image movements, 289, 294, 297, 307, 309
Impregnation (see
infiltration)
Infiltration, 86-92, 94, 95, 109, 117, 124,
355
duration, 87, 355
frozen-dried specimens, 73-74
frozen-substituted specimens, 75-80
Intensity, beam (see beam intensity)
Interference colors, 181, 182, 184-186, 355
Intermediate lens
alignment, 282, 283, 284
Intermediate
solvents
(see
transitional
solvents)
Interpretation
of
micrographs,
321-325
(Figs. 39, 40)
Isobutyric acid solvent, 76
Isopentane freezing bath, 68
j
JUM-5 ultramicrotome, 146
K
Karnovsky's lead hydroxide, 219, 230
Kellenberger, Ryter and Sechaud bacterial
fixation,
42-43
Ketone solvents, 257, 262
Knives for sectioning
(see
glass knives,
diamond knives)
L
Labels
capsules, 89
Laboratory
equipment, 2-6
layout, 1-3
management, 6-8
Lanthanum acetate stain, 78
Lawn's permanganate stain (see
stain,
Lawn's permanganate)
Lead stains (see stains, alkaline lead)
Lead subacetate stain, 231
Leitz ultramicrotome, 143
Lenses (see
particular lenses,
condenser,
objective, intermediate, or projector)
Lever's lead hydroxide, 219, 230
Light green, 261
LKB knife making apparatus, 158
LKB "Ultratome," 131, 140-142
Luft's permanganate fixative, 63-65 (Fig. 5)
Lugol's solution, 261
Luperco CDB catalyst, 97-98, 124
M
Macromolecules, 326, 336, 337, 343, 352
Magnetic fields, 1-2
Magnification, 214, 301-303 (Figs. 35, 36)
Mallory's azure II-methylene blue, 260
Maraglas
embedding, 115-117