372
SUBJECT INDEX
perfusion, 14-15, 23-28, 35, 55-56 (Figs.
3, 12, 39, 40)
Sabatini, Bensch and Barrnett's buffered
fixative,
53
temperature, 25
topical application, 55
Veronal buffer contraindicated, 56
Formvar
films (see supporting films)
replicas, 326, 339, 340, 342, 344
solvents, 198, 200, 207
Fragmentation (see particulate specimens)
Freeze-drying, 68, 71-75
artefacts, 72-74
duration, 73
equipment, 72
suspensions, 331
Freezing, rapid, 67-71, 76
bath, 68-69
glycerinated tissue, 69-71, 76
specimen size, 68
support, 68
Freeze-substitution, 68, 75-80
artefact, 77
autoradiography, 75
duration, 79-80
fixation, 75, 78
histochemistry, 76
solvents, 76
staining, 75, 78
temperature, 76-77
theory, 75-76
Freon, 69
Fresnel fringes (see also diffraction) 292293, 304 (Figs. 34 d-h, 40)
Frozen dried tissue, 61, 67, 72 (Fig. 6)
fixation, 73
histochemistry, 75
infiltration, 73-74
sectioning, 74-75
staining, 75
Fuchsin, 260-261
G
Gelatin
embedding, 107, 128-129
replicas, 341
Giemsa stain, 261
Glass knives
angle, 126, 159-161, 174, 175, 177, 186,
310, 355
best edges, 159-162
breaking, 154-155, 158, 171 (Figs. 14, 15.
16, 17)
contamination, 159, 162, 167, 174-175.
177, 182-184, 186-187
difficulties in manufacture, 157-158
glass for, 149, 157, 158-159
historical, 137
inspection, 161-162
LKB apparatus, 158
manufacture, 149-161
mounting, 170-173, 177, 355 (Fig. 20 a-c)
scoring, 151, 157, 158
tilt, 170-173, 177, 181, 182, 186, 355 (Fig.
20 a-c)
use of, 25
wettability, 93, 115, 162, 174-175, 183
Glutaraldehyde
decomposition, 58
duration of fixation, 57, 58-59, 354
fixation, 20, 51, 52, 57-59, 62, 354 (Fig. 4)
histochemical applications, 51, 57
penetration, 20
purification, 58
storage, 58
Glycerinated tissue, 69-71, 76, 77
Glycogen, 39, 61, 244, 245 (Figs. 5, 12)
Glycol methacrylate (see
also
hydroxy
ethyl methacrylate)
histochemical reactions, 247
sectioning, 124, 174
staining, 217
Glyoxol, 57
Gold for shadowing, 332-333
Golgi apparatus, 15, 47, 101 (Fig. 2)
Gray scale (photographic), 301
Grids (see specimen grids)
Gun
alignment (see also filament, anode, and
condenser lens alignment) 281, 284287, 288 (Fig. 33)
tilt, 284-287 (Fig. 33)
translation, 287, 288
SUBJECT INDEX
perfusion, 14-15, 23-28, 35, 55-56 (Figs.
3, 12, 39, 40)
Sabatini, Bensch and Barrnett's buffered
fixative,
53
temperature, 25
topical application, 55
Veronal buffer contraindicated, 56
Formvar
films (see supporting films)
replicas, 326, 339, 340, 342, 344
solvents, 198, 200, 207
Fragmentation (see particulate specimens)
Freeze-drying, 68, 71-75
artefacts, 72-74
duration, 73
equipment, 72
suspensions, 331
Freezing, rapid, 67-71, 76
bath, 68-69
glycerinated tissue, 69-71, 76
specimen size, 68
support, 68
Freeze-substitution, 68, 75-80
artefact, 77
autoradiography, 75
duration, 79-80
fixation, 75, 78
histochemistry, 76
solvents, 76
staining, 75, 78
temperature, 76-77
theory, 75-76
Freon, 69
Fresnel fringes (see also diffraction) 292293, 304 (Figs. 34 d-h, 40)
Frozen dried tissue, 61, 67, 72 (Fig. 6)
fixation, 73
histochemistry, 75
infiltration, 73-74
sectioning, 74-75
staining, 75
Fuchsin, 260-261
G
Gelatin
embedding, 107, 128-129
replicas, 341
Giemsa stain, 261
Glass knives
angle, 126, 159-161, 174, 175, 177, 186,
310, 355
best edges, 159-162
breaking, 154-155, 158, 171 (Figs. 14, 15.
16, 17)
contamination, 159, 162, 167, 174-175.
177, 182-184, 186-187
difficulties in manufacture, 157-158
glass for, 149, 157, 158-159
historical, 137
inspection, 161-162
LKB apparatus, 158
manufacture, 149-161
mounting, 170-173, 177, 355 (Fig. 20 a-c)
scoring, 151, 157, 158
tilt, 170-173, 177, 181, 182, 186, 355 (Fig.
20 a-c)
use of, 25
wettability, 93, 115, 162, 174-175, 183
Glutaraldehyde
decomposition, 58
duration of fixation, 57, 58-59, 354
fixation, 20, 51, 52, 57-59, 62, 354 (Fig. 4)
histochemical applications, 51, 57
penetration, 20
purification, 58
storage, 58
Glycerinated tissue, 69-71, 76, 77
Glycogen, 39, 61, 244, 245 (Figs. 5, 12)
Glycol methacrylate (see
also
hydroxy
ethyl methacrylate)
histochemical reactions, 247
sectioning, 124, 174
staining, 217
Glyoxol, 57
Gold for shadowing, 332-333
Golgi apparatus, 15, 47, 101 (Fig. 2)
Gray scale (photographic), 301
Grids (see specimen grids)
Gun
alignment (see also filament, anode, and
condenser lens alignment) 281, 284287, 288 (Fig. 33)
tilt, 284-287 (Fig. 33)
translation, 287, 288