SUBJECT INDEX
371
botanical material, 33, 37, 50, 63, 65
cell suspensions, 31-32, 49
cold-blooded animals, 32, 38
criteria of good fixation, 46-49, 62-63
(Fig. 2)
damage, 46-49, 62-63 (Fig. 2)
duration, 20, 46, 55, 57, 58-59, 62, 354
frozen-dried tissue, 73
frozen-substituted tissue, 78
historical, 34-35
human material, 29-31, 51
hyaluronidase pretreatment, 28-29, 30
in situ, 20-23, 45
juvenile tissues, 30-31, 32, 38
minced tissue, 16-20, 29, 45, 354 (Fig. 1)
needle biopsy, 30
perfusion, 14-15, 23-28, 35 (Figs. 3, 12,
39, 40, 51, 55)
pH, 38
post-mortem change, 15-16
secondary (see osmium tetroxide)
specific ions, 38
temperature, 25, 27, 45
tissue exposure, 14-33
topical, 20-23, 29
Fixatives (see
also particular
fixatives)
34-67, 73, 78
acrylic aldehyde (see acrolein)
Afzelius' osmium tetroxide in carbon
tetrachloride, 44-45
aldehyde, 14, 20, 35, 47, 50-63, 67, 122,
123, 124, 126
Bennett and Luft's collidine
buffered
osmium tetroxide, 40-41
Caulfield's isotonic osmium
tetroxide,
37-38
Claude's acidic osmium tetroxide, 44
Dalton's chrome-osmium tetroxide, 4142
formaldehyde (see formalin)
formalin, 20, 22, 23-25, 29, 50-56, 62-63,
101, 107, 354 (Figs. 3, 10, 28, 39, 40)
glutaraldehyde, 20, 51, 52, 57-59, 62,
354 (Fig. 4)
Helander's alkaline osmium
tetroxide,
44
Holt and Hicks' phosphate
buffered
formalin, 50, 53
hydroxyadipaldehyde, 51, 57, 59
Kellenberger, Ryter and Sechaud bacterial method, 42-43
Luft's permanganate, 63-64
Millonig's phosphate buffered
osmium
tetroxide, 39-40, 354 (Figs. 11, 12)
osmium tetroxide, 14, 34, 35-45, 354
Palade's Veronal buffered osmium
tetroxide, 35-37, 39
paraformaldehyde, 52-53
Pease's phosphate
buffered
formalin,
51-52, 354
permanganate, 19, 35, 63-67, 107 (Fig. 5)
Sabatini, Bensch, and Barrnett's buffered aldehydes, 52, 53, 57-59, 62
Tahmisian's isotonic osmium tetroxide,
39
Tahmisian's isotonic permanganate, 65
Tahmisian's permanganate-osmium tetroxide, 44, 67
uranyl acetate, 43
Zetterqvist's isotonic osmium tetroxide,
38-39
Flattening sections, 175-176
Flotation fluid (see trough
fluid)
Focus, 229, 292-293, 303-306, 325 (Figs. 34,
40)
Fog
electron scattering, 265
photographic, 313, 314, 321
Formaldehyde (see
formalin)
Formalin (see also aldehyde) 20, 22, 23-25,
29, 50-56, 62-63, 101, 107, 354 (Figs.
3, 10, 12, 28, 39, 40)
beam damage sensitivity, 101
duration, 58-59, 354
frozen-substituted tissue, 78
histochemical applications, 50, 57
Holt
and Hicks' phosphate
buffered
fixative,
50, 53
methanol-free, 52-53
Pease's phosphate buffered
fixative,
5152, 354
penetration, 20, 29, 56
371
botanical material, 33, 37, 50, 63, 65
cell suspensions, 31-32, 49
cold-blooded animals, 32, 38
criteria of good fixation, 46-49, 62-63
(Fig. 2)
damage, 46-49, 62-63 (Fig. 2)
duration, 20, 46, 55, 57, 58-59, 62, 354
frozen-dried tissue, 73
frozen-substituted tissue, 78
historical, 34-35
human material, 29-31, 51
hyaluronidase pretreatment, 28-29, 30
in situ, 20-23, 45
juvenile tissues, 30-31, 32, 38
minced tissue, 16-20, 29, 45, 354 (Fig. 1)
needle biopsy, 30
perfusion, 14-15, 23-28, 35 (Figs. 3, 12,
39, 40, 51, 55)
pH, 38
post-mortem change, 15-16
secondary (see osmium tetroxide)
specific ions, 38
temperature, 25, 27, 45
tissue exposure, 14-33
topical, 20-23, 29
Fixatives (see
also particular
fixatives)
34-67, 73, 78
acrylic aldehyde (see acrolein)
Afzelius' osmium tetroxide in carbon
tetrachloride, 44-45
aldehyde, 14, 20, 35, 47, 50-63, 67, 122,
123, 124, 126
Bennett and Luft's collidine
buffered
osmium tetroxide, 40-41
Caulfield's isotonic osmium
tetroxide,
37-38
Claude's acidic osmium tetroxide, 44
Dalton's chrome-osmium tetroxide, 4142
formaldehyde (see formalin)
formalin, 20, 22, 23-25, 29, 50-56, 62-63,
101, 107, 354 (Figs. 3, 10, 28, 39, 40)
glutaraldehyde, 20, 51, 52, 57-59, 62,
354 (Fig. 4)
Helander's alkaline osmium
tetroxide,
44
Holt and Hicks' phosphate
buffered
formalin, 50, 53
hydroxyadipaldehyde, 51, 57, 59
Kellenberger, Ryter and Sechaud bacterial method, 42-43
Luft's permanganate, 63-64
Millonig's phosphate buffered
osmium
tetroxide, 39-40, 354 (Figs. 11, 12)
osmium tetroxide, 14, 34, 35-45, 354
Palade's Veronal buffered osmium
tetroxide, 35-37, 39
paraformaldehyde, 52-53
Pease's phosphate
buffered
formalin,
51-52, 354
permanganate, 19, 35, 63-67, 107 (Fig. 5)
Sabatini, Bensch, and Barrnett's buffered aldehydes, 52, 53, 57-59, 62
Tahmisian's isotonic osmium tetroxide,
39
Tahmisian's isotonic permanganate, 65
Tahmisian's permanganate-osmium tetroxide, 44, 67
uranyl acetate, 43
Zetterqvist's isotonic osmium tetroxide,
38-39
Flattening sections, 175-176
Flotation fluid (see trough
fluid)
Focus, 229, 292-293, 303-306, 325 (Figs. 34,
40)
Fog
electron scattering, 265
photographic, 313, 314, 321
Formaldehyde (see
formalin)
Formalin (see also aldehyde) 20, 22, 23-25,
29, 50-56, 62-63, 101, 107, 354 (Figs.
3, 10, 12, 28, 39, 40)
beam damage sensitivity, 101
duration, 58-59, 354
frozen-substituted tissue, 78
histochemical applications, 50, 57
Holt
and Hicks' phosphate
buffered
fixative,
50, 53
methanol-free, 52-53
Pease's phosphate buffered
fixative,
5152, 354
penetration, 20, 29, 56