370
SUBJECT INDEX
Aquon, 122, 123
Araldite, 83, 105, 108-110, 121, 355 (Figs.
3, 4, 5, 29, 39)
capsules, 86, 87, 89, 113, 355
cross-linked methacrylate, 83, 93, 120122, 124-125
desirable characteristics, 92-94
Durcupan, 123
Epon, 83, 110-115, 117, 355 (Figs. 10, 11,
40)
epoxy resins, 83, 86, 88-95, 105, 108-117
flat, 104
frozen-dried specimens, 73-74
frozen-substituted specimens, 77-78
gelatin, 107, 128-129
glycol methacrylate (see
hydroxyethyl
methacrylate)
historical, 82-83, 108, 117, 122-123
hydroxyethyl methacrylate, 123-124
hydroxypropyl methacrylate, 123-125
Maraglas, 115-117
methacrylate, 82-83, 87, 93, 95-107, 124
(Figs. 2, 7, 8, 9, 24, 30, 35, 36, 37, 38)
mixed plastics, 110, 119, 124, 127-128
open-face, 104
Parlodion, 82
polyampholyte,
125-126
polyester resins, 83, 87, 88-95, 107
polystyrene, 127-128
prepolymerized
methacrylate,
103-104,
122, 124, 125 (Fig. 8b)
Selectron, 119-120, 127
Vestopal W, 83, 87, 107, 117-119, 121,
127, 355 (Fig. 12)
Viapale, 120
Endoplasmic reticulum, preservation and
staining, 15, 47, 62, 63, 101 (Fig. 6)
Enlarging
photographic, 314-321 (Fig. 38)
photographic
contrast,
315-318,
321
(Fig. 38)
photographic
development,
314-317
(Fig. 38)
photographic "dodging", 308, 318-320
photographic equipment, 5, 318
photographic
exposure, 315-319,
320321 (Fig. 38)
photographic intensification, 320
photographic papers, 315
Eosin, 261
Epikote 812, 110
Epon
embedding, 83, 110-115, 117, 355
immunological reactions, 249
sectioning, 94, 113-115, 117, 163, 176
(Figs. 10, 11, 40)
staining, 218, 224
thick section staining, 259-262
Epon 562,
113
Epon 812, 110
Epon 815, 113
Epoxy glue, block mounting, 134
Epoxy plastics (see also particular plastics)
embedding, 83, 86, 88-95, 105, 108-117
histochemical reactions, 246
sectioning, 92-95, 174, 175-176
solvents, 218, 257, 262-263
staining, 83, 216, 217, 218, 224, 242
thick section staining, 258-259, 261
toxicity, 91-92
Equipment sources, 360
Etched surfaces, 326
Ethylmethacrylate, 97, 103
Evaporative drying, 68, 71-75
artefacts, 72-74
duration, 73
equipment, 72
F
Farrant and Powell ultramicrotome, 145146
Fernandez-Moran microtome, 143
Ferritin-conjugated antibody stain, 247250
Feulgen reagent, 261
Fibers, 326, 328, 337
Field effects, 307-308
Filament alignment, 281, 282, 284, 287, 288
Films (see supporting films and replicas)
Film nets (see supporting films)
Fixation, 14-67, 73, 78, 354
bacteria, 42-43
barriers, 21, 28
block size, 16-17, 20, 23, 29, 56
SUBJECT INDEX
Aquon, 122, 123
Araldite, 83, 105, 108-110, 121, 355 (Figs.
3, 4, 5, 29, 39)
capsules, 86, 87, 89, 113, 355
cross-linked methacrylate, 83, 93, 120122, 124-125
desirable characteristics, 92-94
Durcupan, 123
Epon, 83, 110-115, 117, 355 (Figs. 10, 11,
40)
epoxy resins, 83, 86, 88-95, 105, 108-117
flat, 104
frozen-dried specimens, 73-74
frozen-substituted specimens, 77-78
gelatin, 107, 128-129
glycol methacrylate (see
hydroxyethyl
methacrylate)
historical, 82-83, 108, 117, 122-123
hydroxyethyl methacrylate, 123-124
hydroxypropyl methacrylate, 123-125
Maraglas, 115-117
methacrylate, 82-83, 87, 93, 95-107, 124
(Figs. 2, 7, 8, 9, 24, 30, 35, 36, 37, 38)
mixed plastics, 110, 119, 124, 127-128
open-face, 104
Parlodion, 82
polyampholyte,
125-126
polyester resins, 83, 87, 88-95, 107
polystyrene, 127-128
prepolymerized
methacrylate,
103-104,
122, 124, 125 (Fig. 8b)
Selectron, 119-120, 127
Vestopal W, 83, 87, 107, 117-119, 121,
127, 355 (Fig. 12)
Viapale, 120
Endoplasmic reticulum, preservation and
staining, 15, 47, 62, 63, 101 (Fig. 6)
Enlarging
photographic, 314-321 (Fig. 38)
photographic
contrast,
315-318,
321
(Fig. 38)
photographic
development,
314-317
(Fig. 38)
photographic "dodging", 308, 318-320
photographic equipment, 5, 318
photographic
exposure, 315-319,
320321 (Fig. 38)
photographic intensification, 320
photographic papers, 315
Eosin, 261
Epikote 812, 110
Epon
embedding, 83, 110-115, 117, 355
immunological reactions, 249
sectioning, 94, 113-115, 117, 163, 176
(Figs. 10, 11, 40)
staining, 218, 224
thick section staining, 259-262
Epon 562,
113
Epon 812, 110
Epon 815, 113
Epoxy glue, block mounting, 134
Epoxy plastics (see also particular plastics)
embedding, 83, 86, 88-95, 105, 108-117
histochemical reactions, 246
sectioning, 92-95, 174, 175-176
solvents, 218, 257, 262-263
staining, 83, 216, 217, 218, 224, 242
thick section staining, 258-259, 261
toxicity, 91-92
Equipment sources, 360
Etched surfaces, 326
Ethylmethacrylate, 97, 103
Evaporative drying, 68, 71-75
artefacts, 72-74
duration, 73
equipment, 72
F
Farrant and Powell ultramicrotome, 145146
Fernandez-Moran microtome, 143
Ferritin-conjugated antibody stain, 247250
Feulgen reagent, 261
Fibers, 326, 328, 337
Field effects, 307-308
Filament alignment, 281, 282, 284, 287, 288
Films (see supporting films and replicas)
Film nets (see supporting films)
Fixation, 14-67, 73, 78, 354
bacteria, 42-43
barriers, 21, 28
block size, 16-17, 20, 23, 29, 56