SUBJECT INDEX
369
specimen, 214-215
troughs, 165, 167, 169, 177, 182-184, 187
uranyl stains, 235
Contrast (see also negative staining and
shadowing)
apertures, effect of, 265, 268, 269, 270
(Fig. 30)
contamination, relation to, 306
diffraction
phenomena,
304-306,
325
(Figs. 34 a-c, 40)
embedment, effect of, 82-83, 105, 107,
115, 118, 127-128
fluorescent
image, 303, 304
focus, relation to, 303-304, 325 (Figs.
34 a-c, 40)
photographic, 310-321 (Figs. 37, 38)
photographic developer, 313-314
photographic papers, 315
photographic plates, 310-311
replicas, 326
section thickness, 185, 301-303, 309 (Figs.
35, 36)
supporting films, 192, 193, 199, 206, 211
(Fig. 24)
Criteria of good fixation, 47-49, 62-63,
(Fig. 2)
Critical point drying, 331
Cross-linked methacrylate embedding, 93,
121 (Fig. 28)
"Cross-over" illumination, 269, 270, 274,
285, 288, 307, 320
Crotonaldehyde, 57, 78
Crystal violet, 260
Current alignment, 282-283, 284
Cytomembranes, 15, 39, 47, 49, 61, 63, 66,
67, 72, 101, 234, 237, 244, 326, 356
(Figs. 4, 5, 6, 8, 12, 28, 29, 39)
D
Dalton and Zeigel's lead staining technique, 230-231
Dalton's chrome-osmium tetroxide, 41-42
Dehydration, 84-87, 94
duration, 84
evaporative drying, 68, 71-75
permanganate fixed tissue, 66
substitution in frozen material, 68, 75-80
temperature control, 84
ultra-rapid, 66
Densitometer, 5, 319
Developers, photographic, 313-315, 321
Diamond knives
angle, 162
cleaning, 163
sources, 164-165
trough fluid, 163
use of, 115, 125, 149
wettability, 115, 162, 174
wetting agents, 163
Dibutyl phthalate, plasticizer, 105, 108,
115, 118
Dichlorobenzoyl peroxide, catalyst, 97
Dichromate buffer, 41-42
Diffraction (see also Fresnel fringes)
astigmatism
correction, 292-293
(Fig.
34 d-k)
bands, 292-293, 304-306, 325 (Figs. 34
d-h, 40)
contrast, relation to, 304-306, 325 (Figs.
34 a-c, 40)
focusing, used for, 304-306, 325 (Figs.
34, 40)
image effects, 325
Dimethylaminoethylmethacrylate
monomer, 126
Dimethyl formamide, solvent, 76, 85
Dimethyl sulfoxide, solvent, 71
Dioxane, solvent, 76, 85, 258
Dispersions (see particulate specimens)
Divinyl benzene, cross-linking agent, 83,
93, 120-122, 124-125
DMP-30 accelerator, 90, 91, 108-109, 111
Dodecenyl succinic anhydride, hardener,
108, 111
"Dodging" prints, 308, 318-320
Drift (see also movement)
294, 307, 309 (Fig. 34)
Durcupan, 110, 123
Durcupan ACM, 108
E
Elastin stains, 236, 245 (Fig. 29)
Embedding (see also particular plastics)
82-135
369
specimen, 214-215
troughs, 165, 167, 169, 177, 182-184, 187
uranyl stains, 235
Contrast (see also negative staining and
shadowing)
apertures, effect of, 265, 268, 269, 270
(Fig. 30)
contamination, relation to, 306
diffraction
phenomena,
304-306,
325
(Figs. 34 a-c, 40)
embedment, effect of, 82-83, 105, 107,
115, 118, 127-128
fluorescent
image, 303, 304
focus, relation to, 303-304, 325 (Figs.
34 a-c, 40)
photographic, 310-321 (Figs. 37, 38)
photographic developer, 313-314
photographic papers, 315
photographic plates, 310-311
replicas, 326
section thickness, 185, 301-303, 309 (Figs.
35, 36)
supporting films, 192, 193, 199, 206, 211
(Fig. 24)
Criteria of good fixation, 47-49, 62-63,
(Fig. 2)
Critical point drying, 331
Cross-linked methacrylate embedding, 93,
121 (Fig. 28)
"Cross-over" illumination, 269, 270, 274,
285, 288, 307, 320
Crotonaldehyde, 57, 78
Crystal violet, 260
Current alignment, 282-283, 284
Cytomembranes, 15, 39, 47, 49, 61, 63, 66,
67, 72, 101, 234, 237, 244, 326, 356
(Figs. 4, 5, 6, 8, 12, 28, 29, 39)
D
Dalton and Zeigel's lead staining technique, 230-231
Dalton's chrome-osmium tetroxide, 41-42
Dehydration, 84-87, 94
duration, 84
evaporative drying, 68, 71-75
permanganate fixed tissue, 66
substitution in frozen material, 68, 75-80
temperature control, 84
ultra-rapid, 66
Densitometer, 5, 319
Developers, photographic, 313-315, 321
Diamond knives
angle, 162
cleaning, 163
sources, 164-165
trough fluid, 163
use of, 115, 125, 149
wettability, 115, 162, 174
wetting agents, 163
Dibutyl phthalate, plasticizer, 105, 108,
115, 118
Dichlorobenzoyl peroxide, catalyst, 97
Dichromate buffer, 41-42
Diffraction (see also Fresnel fringes)
astigmatism
correction, 292-293
(Fig.
34 d-k)
bands, 292-293, 304-306, 325 (Figs. 34
d-h, 40)
contrast, relation to, 304-306, 325 (Figs.
34 a-c, 40)
focusing, used for, 304-306, 325 (Figs.
34, 40)
image effects, 325
Dimethylaminoethylmethacrylate
monomer, 126
Dimethyl formamide, solvent, 76, 85
Dimethyl sulfoxide, solvent, 71
Dioxane, solvent, 76, 85, 258
Dispersions (see particulate specimens)
Divinyl benzene, cross-linking agent, 83,
93, 120-122, 124-125
DMP-30 accelerator, 90, 91, 108-109, 111
Dodecenyl succinic anhydride, hardener,
108, 111
"Dodging" prints, 308, 318-320
Drift (see also movement)
294, 307, 309 (Fig. 34)
Durcupan, 110, 123
Durcupan ACM, 108
E
Elastin stains, 236, 245 (Fig. 29)
Embedding (see also particular plastics)
82-135