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The development of chemical xationtechniques for electron microscô‘py hâä“lëaätô
kable advances in our understanding of
Cells. ‘The
suc—
cessively, of osmium tetr0Xid@
xatiÿesg‘epbw resms
oÎf
hyde xation has generated -a Ïcdndëcein present methods WhiCh‘is'
jiiëtiëd
by ancillary techniques of
di‘ractioh, polarization
isolati0n studies. However, în
eggs,
certain plant cells, certainprot020à, '
inie‘
,caohical techmquesoften result
in very poorly xed
must be
results are obtained. The criteria for
X‘ation,‘ however, canhardlÿfbe dened Within
the eld of
s1mple
seen depending upon
ma&ræ>ptæ; osmolaritÿ}lèëthbfîät‘iô;
temperature, etc. One is thus faœd With the dilèmma that one adjusts variables until a
morphology is obtained which is “acceptable” to othenorphologistS-. in the
components such ‘as mitochondria, golgî, endoplasmic,reticum, etc.,’resemb‘le those seen
in “well—xed” material.
it has lt}îèÿthorough disad—
«
vantage of creating “schools” of
xation _and'ign‘ofingïthe
of Xati0hï
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i.e., the deliberate and reproduciblè creation
WhlCh
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Our work in rapid *ee'zigÎbegan asasearchforatechmqueot‘locmg at=cells Wh10h
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