TIŒRMAL DEGRADA‘IÏON OF VIRUSES
‘
117
of thermal degradation that3arè “identical except for changesistaæ; changes in composition, etc. i.e. dried suspensions of virus vs. liquid suspensions of virus; virus suspensions
with additives‘vs. virus suspensions Without additive, etc.
‘
,
’
,
The reaction constants or rates of degradation (kfs)
of preparatiosnf viruses (equation4
B) can be usedfor determining the
heat of activation ,.(ÆH*) and the-entropy of activati0n (AS*)by«substitüti‘ons in and
simultàn‘eous' solution-of equation 8.Free energy, can be Calculated by'"using the—"values
abo'veæ in“: eq11'a‘ti0n ?9.
obtained» are shown in‘ Table: 4.1 «;
=
,
€
,
TABLE 4. “T1ËmqDÿNmc,_
qu0”m On'_ÏDR1ED PŒDÆÀnONS
‘
OF SUSPENSIONS OF MEASLES VIRUS ÏN. MEDIÙM_19_9 DLDS”PARIŒ, DAVIS ADD'IVES
Tem‘êäm
TÊpÎŒ
AH*
AS*
MH
;
(°C)
,
.
:ÇÇal"ï°l‘?/°Ç?
,5Ça1/11}01°)
‘
,
'
,
,
,
16.8; 24.0
.
.
.
.
.
‘.
.
.
”20.4“
{f‘î’î
f* ”43.073
”"’—83:51
[ 16.8; 32.8
.
.
.
.
.
.
.
.
"24.8
“
29;4ë$“‘“
16.8; 42.0
.
.
.
.
.
.
.
.
29.4
33.0? ' _
24.0; 32.8
.
.
.
.
.
.
.
.
28.4
‘i7,7‘71
'
"
'
16.0“
'
18253
24.0; 42.0
.
.
.
.
.
‘.
.
.
33.0
22,061
_
-
12.8
18144
32.8; 42.0
.
.
.
.
.
.
.
.
_
37.4
26,413
_
Î26.6
‘
»
,
—.
DriedkSus1‘a'æsîoris
.:;sgç-xgë- -;
;...
'
28.0; 36.0
.
.
.
.
.
.
.
.
32.0
22,743
13.2
"18,715
28.0; 45.0 . . . . .". .. .
‘
'
36.5
]…
—113’18:860
36.0; 45.0 '.
.
.
f
…40.5
8;0971
..:
and proteins bythennälùæÿaôn 1sassocrated,m general,
With large poSitiVe7Values of
large values ofAH*cannot
accounted for in:-terms of ’a ‘
single
values
obtained are duetothebreaking'ofmanÿ0heimcalbonds‘,mostprobablymsegfhydmgen ,
_
Whenïsui'cie‘nt— numbers of
moleculeofprotemmno -
rmlyto1tsnat1veconguraüon,thecollapseofstructure resultmgmalargg
},
increase inættop'y. ÊAlthough..theheatofact1vaüon(AH*)appearstObethe1mportant
‘
f“
valuef0r TAS*
-Ï*i ;.
‘nuc1äeâcidäÇDNAä Thesestud1esfürmshed additional—evidencesthatite‘2a’C‘cfual,«mecham3m
‘
117
of thermal degradation that3arè “identical except for changesistaæ; changes in composition, etc. i.e. dried suspensions of virus vs. liquid suspensions of virus; virus suspensions
with additives‘vs. virus suspensions Without additive, etc.
‘
,
’
,
The reaction constants or rates of degradation (kfs)
of preparatiosnf viruses (equation4
B) can be usedfor determining the
heat of activation ,.(ÆH*) and the-entropy of activati0n (AS*)by«substitüti‘ons in and
simultàn‘eous' solution-of equation 8.Free energy, can be Calculated by'"using the—"values
abo'veæ in“: eq11'a‘ti0n ?9.
obtained» are shown in‘ Table: 4.1 «;
=
,
€
,
TABLE 4. “T1ËmqDÿNmc,_
qu0”m On'_ÏDR1ED PŒDÆÀnONS
‘
OF SUSPENSIONS OF MEASLES VIRUS ÏN. MEDIÙM_19_9 DLDS”PARIŒ, DAVIS ADD'IVES
Tem‘êäm
TÊpÎŒ
AH*
AS*
MH
;
(°C)
,
.
:ÇÇal"ï°l‘?/°Ç?
,5Ça1/11}01°)
‘
,
'
,
,
,
16.8; 24.0
.
.
.
.
.
‘.
.
.
”20.4“
{f‘î’î
f* ”43.073
”"’—83:51
[ 16.8; 32.8
.
.
.
.
.
.
.
.
"24.8
“
29;4ë$“‘“
16.8; 42.0
.
.
.
.
.
.
.
.
29.4
33.0? ' _
24.0; 32.8
.
.
.
.
.
.
.
.
28.4
‘i7,7‘71
'
"
'
16.0“
'
18253
24.0; 42.0
.
.
.
.
.
‘.
.
.
33.0
22,061
_
-
12.8
18144
32.8; 42.0
.
.
.
.
.
.
.
.
_
37.4
26,413
_
Î26.6
‘
»
,
—.
DriedkSus1‘a'æsîoris
.:;sgç-xgë- -;
;...
'
28.0; 36.0
.
.
.
.
.
.
.
.
32.0
22,743
13.2
"18,715
28.0; 45.0 . . . . .". .. .
‘
'
36.5
]…
—113’18:860
36.0; 45.0 '.
.
.
f
…40.5
8;0971
..:
and proteins bythennälùæÿaôn 1sassocrated,m general,
With large poSitiVe7Values of
large values ofAH*cannot
accounted for in:-terms of ’a ‘
single
values
obtained are duetothebreaking'ofmanÿ0heimcalbonds‘,mostprobablymsegfhydmgen ,
_
Whenïsui'cie‘nt— numbers of
moleculeofprotemmno -
rmlyto1tsnat1veconguraüon,thecollapseofstructure resultmgmalargg
},
increase inættop'y. ÊAlthough..theheatofact1vaüon(AH*)appearstObethe1mportant
‘
f“
valuef0r TAS*
-Ï*i ;.
‘nuc1äeâcidäÇDNAä Thesestud1esfürmshed additional—evidencesthatite‘2a’C‘cfual,«mecham3m