100
FREEZE—‘DRYING‘
_
.TAÈLE
SURVIVAL IMMEDÏATELY AFTER; ÈRYING s. CEREVISIAE
…
_
__
‘
‘
“
IN DIFFERENT
DR_YING
MEDIA
‘
‘
‘
”
'
‘Perœntage survival immediately '
’
_
‘
»
_
"
after drying at :
.
Suspending meghum
___________
«
.
—12.5 °C
—-25 °C
—35° c
ÎDistiiled
.
.
.
.
.
.
.
.
.
‘1
19
_
-18
_,
.
.’
.
._
_
71
70
85
SucroselZS/
.
.
63 '_
72
_
'
PeptonelO%
63
‘
‘ë3
‘
‘PVPIO%................
4
50
49
Glucose 7.5 %
PeptoneS%
70
100
85
PVP 10 %
_
Sucrose 10% —
‘
‘î‘
'
_
Nag1utamateZ%
.
.
.
.
.,
.
.
.
.
r71x]
;
*
685
,
_
—
74
PVP 10%
…
_
Though thé results varÿ‘ ii1 defail, the gehefal trend is for survival to improve a_s_the
of=..dr_yipg gçt_s lower.
two:_
glucose_and sucrqse, and_ peptone,
all show considérable. Pf°tective
how WÇ11:P- VP proteets
againstlfreeZ_ging…injurya tel?
a diving.proteçvç
comparativelÿpoor»
'
_
BQW
prçyi9uslybæn
advocated as drying media.
'
usedsmgly
…
:
- _
“
Another organism we “have used is astram0fP-Ÿe”dom0naSpyacyanea
_ us
”“bÿ*Dr.’Ashwood
smith.
Unde‘r
% sucro‘se at—- 35 °C,
.;
a-‘Survivä1’0f
25 % reS'u1ted,“ Wh1Ch d'r01‘3ped to_ 15*%
'Hçwevè‘r, in 10 % peptone,
.
thesurV1vai
añd"ihaWig‘—Was”47'% and
43 %. ”This appears to be
anorgamsmwhmhisvery sènsitiVe ”to freezmghutfa1yres1stant to drÿing.
'
,
pïçqæèti@n “against both f‘çezîng
'
_
anddrymgmwryWeareatpresemmvesügætügtheEcolphage ÎT4 “Whiä' is“yery
_
sens1üvetofreezmgandagamthhtmsorgamsm10/peptonegwesremarkab1eprotect…
”
agamstfreezmg1mury
.
—anoaŒdsW5haVÔbeenœStlgpuœSlgleam10301dsfŒpIotecüveacüonSome
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