400
380
Excitation Wavelength, nm
360
340
320
300
280
260
300
350
400
450
500
550
600
300
350
400
450
Emission Wavelength, nm
500
550
600
300
350
400
450
Emission Wavelength, nm
500
550
600
300
350
400
450
500
550
600
240
400
380
Excitation Wavelength, nm
360
340
320
300
280
260
240
400
380
360
340
320
300
280
260
240
400
H
N
OH
H 3 C
380
360
340
320
300
280
260
240
(a)
(b)
(d)
(c)
Plate 3. Excitation–emission spectra for (a) bovine serum albumin, (b) indole, (c) cresol, and (d)
rhodendron tannin.