64
Biospherical Instruments in San Diego, California (Fig. 2; courtesy M.M. Tilzer). Our effort
to expand our sets of Chlorophyll a measurements to the detection of other chlorophylls and
carotenoids was stimulated by G.W. Hallegraeff, then at Amsterdam University, who
routinely used thin-layer chromatography, albeit not to eventually relate pigment
.80r-----------------------------------~
450
110m depth
500
550
600
wavelength (nm)
o
1
650
700
15m depth
Figure 3. Absorption spectra (in acetone) of Chi a, Chi b, and the picoplanktonic Prochlorophyte's
"divinyl-Chl a" and "divinyl- Chi b", both red-shifted. 2-d thin-layer chromatograms are also shown
(samples taken near surface and at 110 m, at the depth of the deep chlorophyll maximum in the central
tropical Atlantic, November 1977). 1. Origin (olive-brown) 2. Butanoyloxyfucox (yellow). 3. Fucoxanthin
(yellow). 4. Hexanoyloxy-fucoxanthin (yellow). 5. Unknown. 6. Unknown (yellow-orange). 7. DivinylChi b (brown-green). 8. Chi b (olive-green). 9. Divinyl-Chl a (green). 10. Chi a (blue-green). 11.
Zeaxanthin. 12. Carotene (orange). 13. Secondary carotenoid (bright yellow). 14. Secondary carotenoid
(pink).
concentrations to wavelength-dependent absorption and photophysiological properties of
phyto-plankton, which was our objective, but merely to study succession in the taxonomic
composition of natural freshwater lake populations (Hallegraeff, 1977).
Biospherical Instruments in San Diego, California (Fig. 2; courtesy M.M. Tilzer). Our effort
to expand our sets of Chlorophyll a measurements to the detection of other chlorophylls and
carotenoids was stimulated by G.W. Hallegraeff, then at Amsterdam University, who
routinely used thin-layer chromatography, albeit not to eventually relate pigment
.80r-----------------------------------~
450
110m depth
500
550
600
wavelength (nm)
o
1
650
700
15m depth
Figure 3. Absorption spectra (in acetone) of Chi a, Chi b, and the picoplanktonic Prochlorophyte's
"divinyl-Chl a" and "divinyl- Chi b", both red-shifted. 2-d thin-layer chromatograms are also shown
(samples taken near surface and at 110 m, at the depth of the deep chlorophyll maximum in the central
tropical Atlantic, November 1977). 1. Origin (olive-brown) 2. Butanoyloxyfucox (yellow). 3. Fucoxanthin
(yellow). 4. Hexanoyloxy-fucoxanthin (yellow). 5. Unknown. 6. Unknown (yellow-orange). 7. DivinylChi b (brown-green). 8. Chi b (olive-green). 9. Divinyl-Chl a (green). 10. Chi a (blue-green). 11.
Zeaxanthin. 12. Carotene (orange). 13. Secondary carotenoid (bright yellow). 14. Secondary carotenoid
(pink).
concentrations to wavelength-dependent absorption and photophysiological properties of
phyto-plankton, which was our objective, but merely to study succession in the taxonomic
composition of natural freshwater lake populations (Hallegraeff, 1977).