18
Materials and Methods
75° 30' W
73° 3Q' W
71 6 30'W
69° 3Q' W
676 3Q' W
Fig. 201. Zooplankton sampling sites during the R/V Cariboo Cruise in the Straits of Magellan in FebruaryMarch 1991
A1-A2
antennae 1-2
B1-B2
basal segments 1-2
P1-P4
swimming legs 1-4
Ri
endopod
Re
exopod
Ri1-Ri3
endopod segments (joints) 1-3
Rel-Re3
exopod segments (joints) 1-3
Th5
fifth thorax somite
All specimen measurements were made
under a dissecting microscope with an ocular
micrometer. The cephalothorax length is
considered to be the distance between the
anterior tip of the forehead and the posterior
margin of the intersegmental membrane lying
between the last metasomal somite and the
genital somite. The abdomen length is the
distance between the mid-dorsal anterior
margin of the genital somite and the distal
end of the caudal ramus. These lengths were
used to determine both body length and proportional lengths of the cephalothorax and
abdomen.
Parts were dissected from the animals and
were permanently mounted in glycerine.
Drawings of the body and appendages were
made with the use of a camera lucida
mounted on an Axiophot Zeiss microscope.
Original ink figures were then processed by a
computer graphic system (Apple Macintosh
Quadra with 20 MB RAM and 128 MB
magneto-optic disk). The images were
scanned through a UMAX 600 DP! scanner
and then proportionally reduced and paged
through an Aldus Free Hand drawing
program.
2.3 Scanning Electron Micrographs
Preserved specimens were washed twice in
distilled water and then left in distilled water
for another 24h. The next day, they were
Materials and Methods
75° 30' W
73° 3Q' W
71 6 30'W
69° 3Q' W
676 3Q' W
Fig. 201. Zooplankton sampling sites during the R/V Cariboo Cruise in the Straits of Magellan in FebruaryMarch 1991
A1-A2
antennae 1-2
B1-B2
basal segments 1-2
P1-P4
swimming legs 1-4
Ri
endopod
Re
exopod
Ri1-Ri3
endopod segments (joints) 1-3
Rel-Re3
exopod segments (joints) 1-3
Th5
fifth thorax somite
All specimen measurements were made
under a dissecting microscope with an ocular
micrometer. The cephalothorax length is
considered to be the distance between the
anterior tip of the forehead and the posterior
margin of the intersegmental membrane lying
between the last metasomal somite and the
genital somite. The abdomen length is the
distance between the mid-dorsal anterior
margin of the genital somite and the distal
end of the caudal ramus. These lengths were
used to determine both body length and proportional lengths of the cephalothorax and
abdomen.
Parts were dissected from the animals and
were permanently mounted in glycerine.
Drawings of the body and appendages were
made with the use of a camera lucida
mounted on an Axiophot Zeiss microscope.
Original ink figures were then processed by a
computer graphic system (Apple Macintosh
Quadra with 20 MB RAM and 128 MB
magneto-optic disk). The images were
scanned through a UMAX 600 DP! scanner
and then proportionally reduced and paged
through an Aldus Free Hand drawing
program.
2.3 Scanning Electron Micrographs
Preserved specimens were washed twice in
distilled water and then left in distilled water
for another 24h. The next day, they were