492
Subject Index
Orientation 263
Orientation of repeat variants 467
OUo's solution 99, 128
P
PAC clones 303
Pachytene chromosome 330
Pachytene nucleus 338
Padlock probes 24
Painting probes 403, 447
- Partial painting probes 403
Pallister-Killian syndrome 97
Paracentric inversion 419
Paraffin-embedded 148, 149
- Paraffin embedded tissue 162
PCR 373,374
Pellet 121
Peripheral blood 73,90,410
Peripheral blood lymphocytes 75
Peroxidase system 18
Philadelphia chromosome 31, 34
Phosphate buffer 392
Photomultiplier tube 347
Photon accumulation 349
PI 258
Pixel 54
- Pixel shift 57,63
Placenta 155
Polymerase chain reaction (PCR) 474
Polypeptide nucleic acids 24
- PNA 24
Polyploidy 155
Polystyrene materials 260
Positional cloning 38
Positively charged slides 150
Post fIxation 257
PostflXation 420
Post hybridzation washes 103, 133,316,
400,417
Post-hybridization 118
POU5Fl 479
Prader-Willi syndrome 30
PreamplifIcation 394, 396
Preannealing 295
Predigestion 447
Preimplantation genetic diagnostic
(PGD) 31
Prenatal 402
Prenatal diagnosis 363
Preparation of high molecular weight
(HMW) DNA 234, 235
Preparation of
- Buccal smears 101
- Urinary cells 102
Preparing high quality slides is one of
the most critical steps 447
Preparing specimen slides 151
Pre-sporulation medium 331
Pretreatment 97, 143, 293, 414, 420
Primary fIbroblast cultures 315
Primed in situ labelling (PRINS) 14
- PRINS-CGH 470
- PRINS-FISH 469,470
- PRINS-painting 470
- Quantitative PRINS 465
Primer extension 476
Probe complexity 475
Probe specifIty 421
Probes 240,372,416
- Cloning vector-specifIc 241
- labeling via random priming 242
- Whole chromosome painting
probe 372, 383
- Locus-specifIc probes 372, 376
- Preparation of 375
- Fluorochrome labeling scheme 376
- Hybridization and detection of 377
Proteolytic digestion 9
Proteolytic enzyme 399
- Pepsin 129, 399
- Proteinase K
Proto-oncogen 157
Pseudocolor 349
Pseudomosaicism 116
Pulsed fIeld gel electrophoresis (PFGE)
234,235,249,373
PurifIcation 397
Q
Quadriperm trays 99, 128
Quantitative analysis 354
Quantitative detection reaction 465
Quantitative staining 463
R
Radiation 408
Radiation cytogenetics 291
Subject Index
Orientation 263
Orientation of repeat variants 467
OUo's solution 99, 128
P
PAC clones 303
Pachytene chromosome 330
Pachytene nucleus 338
Padlock probes 24
Painting probes 403, 447
- Partial painting probes 403
Pallister-Killian syndrome 97
Paracentric inversion 419
Paraffin-embedded 148, 149
- Paraffin embedded tissue 162
PCR 373,374
Pellet 121
Peripheral blood 73,90,410
Peripheral blood lymphocytes 75
Peroxidase system 18
Philadelphia chromosome 31, 34
Phosphate buffer 392
Photomultiplier tube 347
Photon accumulation 349
PI 258
Pixel 54
- Pixel shift 57,63
Placenta 155
Polymerase chain reaction (PCR) 474
Polypeptide nucleic acids 24
- PNA 24
Polyploidy 155
Polystyrene materials 260
Positional cloning 38
Positively charged slides 150
Post fIxation 257
PostflXation 420
Post hybridzation washes 103, 133,316,
400,417
Post-hybridization 118
POU5Fl 479
Prader-Willi syndrome 30
PreamplifIcation 394, 396
Preannealing 295
Predigestion 447
Preimplantation genetic diagnostic
(PGD) 31
Prenatal 402
Prenatal diagnosis 363
Preparation of high molecular weight
(HMW) DNA 234, 235
Preparation of
- Buccal smears 101
- Urinary cells 102
Preparing high quality slides is one of
the most critical steps 447
Preparing specimen slides 151
Pre-sporulation medium 331
Pretreatment 97, 143, 293, 414, 420
Primary fIbroblast cultures 315
Primed in situ labelling (PRINS) 14
- PRINS-CGH 470
- PRINS-FISH 469,470
- PRINS-painting 470
- Quantitative PRINS 465
Primer extension 476
Probe complexity 475
Probe specifIty 421
Probes 240,372,416
- Cloning vector-specifIc 241
- labeling via random priming 242
- Whole chromosome painting
probe 372, 383
- Locus-specifIc probes 372, 376
- Preparation of 375
- Fluorochrome labeling scheme 376
- Hybridization and detection of 377
Proteolytic digestion 9
Proteolytic enzyme 399
- Pepsin 129, 399
- Proteinase K
Proto-oncogen 157
Pseudocolor 349
Pseudomosaicism 116
Pulsed fIeld gel electrophoresis (PFGE)
234,235,249,373
PurifIcation 397
Q
Quadriperm trays 99, 128
Quantitative analysis 354
Quantitative detection reaction 465
Quantitative staining 463
R
Radiation 408
Radiation cytogenetics 291