Ligation step 25
Light emitting diode (LED) 348
Lipsol 331,336
Locked nucleic acids 24
- LNA 24
Long range PCR 338
Lymphocytes 73, 144,381,382,383
Lymphomas 155, 157
M
MAC 39
- morphology, antibody, chromosome
39
Magnification 53
Mammalian ancestor 311
Marker chromosome 393
Maternal cell contamination 124
MCLl 479
Meningioma 178
Mental retardation 32
Metaphase production 204
Metaphase spreads 206, 369, 370
- analysis 380
- Sky analysis of 381, 383
MFISH 63
Microbiology 37
Micro-cloning 388
Microdeletion 82
Microdeletion syndromes 29
Microdissected Cells 185
Microdissection 13, 189, 200, 388
Microgel 282, 284
Micrometric system 348
Mineral oil 393
Mixed base oligonucleotide primed PCR
241
Modifiying Enzymes 273, 275
Monosomy 22 178
Monosomy 22ql1.2
(DiGeorge syndrome) 107
Morphological structure 349
Mosaicism 30,97, 104, 109, 116, 156
Mounting 210
Mounting media 411
Mounting solution 123
Mouse embryonic fibroblasts 205
Mouse metaphase 204
mRNA FISH 171
Multi color banding (mBand) 63,405
Multicolor FISH 408
Multicolor PRINS 468, 469
Multifluor FISH 19
Multi-lobulated nuclei 124
Multiple band filter 58
Mutagens 135
Myotonic dystrophy 466
N
Natural nucleic acids 5
Neutrophils 124
Nick translate 423
Nick translation 14, 294, 325
No hybridization signals 459
No staining/high background staining
459
Nonneoplastic tissue 198
Normal controls 104
Normalized ration 478
Nuchal translucency 115
Nuclear morphology 124
Nuclear spreading 335
Nuclease (NU) 273
- Nuclease Bal 31 276
- Nuclease degradation 396
nucleoids 336
Nucleotide analogues 266
Nucleus extraction 176
NU-FISH 272,273
- FISH 273
- FISH after NU 276
Number of cells 447
Numerical aperture (N. A.) 52, 58
Numerical chromosomal aberrations 97
o
Objectives 443
Oligonucleotide probes 463
Oligonucleotides 11
On-chip integration 60
Oncogene 481
On-screen focussing 62
Optical aberrations 68
Optical path 58
Optical resolution 51
Optical resolution limit 52
Optimal pepsin concentration and
incubation duration 448
Oral mucosa 98
Subject Index
491