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ILSE CHUDOBA and GABRIELE SENGER
Fig. 6. A Gallery of the six color channels captured with a single band pass filter each together with the resulting mBA D pattern. The prof1Ies represent the fluorescent intensities
along the chromosome. B, C Examples for chromosomes 5 at different stages of condensation which show the same mBA D pattern resolution
bridized together. Due to the overlapping of probes next to each other, a
multitude of different fluorescence intensity ratios is obtained (Fig. 6). A
specific feature of the ISIS/mFISH software from MetaSystems (Germany),
calculates the fluorescence intensity ratios within defined regions and assigns a false color. These calculations lead to the characteristic color banding (Fig. 6). Repeated hybridizations proved that this procedure is highly
reproducible. Moreover, comparing investigations revealed that the banding pattern is largely independent of chromosome condensation and the
same banding pattern can be obtained on chromosomes of different
lengths (Fig. 6).