310
HARRY SCHERTHAN
tool to elucidate karyotypic rearrangements in primate evolution (for review see Wienberg and Stanyon 1997). However, the application of comparative chromosome painting to species combinations spanning different mammalian orders failed initially. Since it was likely that the concentration of informative (conserved) DNA molecules contributing to a paint
signal will diminish with increasing evolutionary distance of two species
under investigation, it was supposed that only highly concentrated probes
prepare highly concentrated
paint pr be
~
denature
obtain metapha e preads
from pecies f choice
~
denature
..... ./"--- __0
7'
hybridize for >4 day. at 37°C
peJform tluore. cent detection
Scheme I. Outline of the strategy applied in Zoo-FISH mapping. As compared to standard
chromosome painting, extended hybridization time and highly concentrated DNA probes
are requisite to achieve labeling at segments of conserved synteny
HARRY SCHERTHAN
tool to elucidate karyotypic rearrangements in primate evolution (for review see Wienberg and Stanyon 1997). However, the application of comparative chromosome painting to species combinations spanning different mammalian orders failed initially. Since it was likely that the concentration of informative (conserved) DNA molecules contributing to a paint
signal will diminish with increasing evolutionary distance of two species
under investigation, it was supposed that only highly concentrated probes
prepare highly concentrated
paint pr be
~
denature
obtain metapha e preads
from pecies f choice
~
denature
..... ./"--- __0
7'
hybridize for >4 day. at 37°C
peJform tluore. cent detection
Scheme I. Outline of the strategy applied in Zoo-FISH mapping. As compared to standard
chromosome painting, extended hybridization time and highly concentrated DNA probes
are requisite to achieve labeling at segments of conserved synteny