23 Detection of Radiosensitivity by Means of Three-Color FISH
299
dose dependance of radiation induced aberrations. The avarage number
of breaks indicates a moderate increase in in vitro radiosensitivity (see
Figure 2). Occasional overlaps of aberration frequencies were observed
only between AT-homozygotes and AT-heterozygotes. However, these
two groups can be differentiated by the quality of aberrations, that is
by the number of complex aberrations (data not shown). Complex aberrations are observed in a higher frequency in patients with increased
radiosensitivity. AT-heterozygotes show less complex aberrations than
AT-homozygote gene carriers.
Thus, using the three-color-FISH approach, two independent evaluation criteria are available to detect radiosensitivity in patients. Within a
few days, an estimation of individual radiosensitivity can be achieved. In
conclusion the three-color-FISH technique can be proposed as a predictive assay for detecting radiosensitive patients.
Acknowledgements. The author thanks Dr. M. Stumm, Magdeburg, and Dr. T. Dork, Hannover, for providing AT homozygote and heterozygote lyrnphoblastoid cell lines.
References
Barrios, L, Mir6, R, Caballin, MR, Fuster, C, Guedea, F, Subias, A, Egozcue, J (1989)
Cytogenetic effects of Radiotherapy. Breakpoint Distribution in induced chromosome aberrations. Cancer Genet Cytogenet 41, 61-70
Cigarran, S, Barrios, L, Barquinero, J, F, Caballin, MR, Ribas, M, Egozcue, J (1998)
Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations, analysed by painting. Int JRadiat
BioI 74, 449-455.
Gebhart, E, Neubauer, S, Schmitt, G, Birkenhake, S, Dunst J(1996) Use of a three-colour chromosome-in situ-suppression (CISS) technique for the cytogenetic detection
of past radiation exposure. Radiat Res 145, 47-52.
Greulich, KM, Kreja L, Heinze, B, Rhein, AP, Weier, HG, Bruckner, M, Fuchs P, Molls,
M (2000) Rapid detection of radiation-induced chromosomal aberrations in lymphocytes and hematopoietic progenitor cells by mFISH. Mutat Res 452, 73-81.
Lichter, P, Cremer, T, Borden, J, Manuelidis, L, Ward, DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80, 224-234.
Natarajan, AT, Vyas, RC, Darroudi, F, Vermeulen, S (1992) Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by
in situ hybridization using chromosome-specific DNA libraries. Int JRadiat BioI 61,
199-203.
Neubauer, S, Gebhart, E, Schmitt, G, Birkenhake, S, Dunst J (1996) Is chromosome in
situ suppression (CISS) hybridization suited as a predictive test for intrinsic radiosensitivity in cancer patients? Int J Oncol 8, 707-712.
299
dose dependance of radiation induced aberrations. The avarage number
of breaks indicates a moderate increase in in vitro radiosensitivity (see
Figure 2). Occasional overlaps of aberration frequencies were observed
only between AT-homozygotes and AT-heterozygotes. However, these
two groups can be differentiated by the quality of aberrations, that is
by the number of complex aberrations (data not shown). Complex aberrations are observed in a higher frequency in patients with increased
radiosensitivity. AT-heterozygotes show less complex aberrations than
AT-homozygote gene carriers.
Thus, using the three-color-FISH approach, two independent evaluation criteria are available to detect radiosensitivity in patients. Within a
few days, an estimation of individual radiosensitivity can be achieved. In
conclusion the three-color-FISH technique can be proposed as a predictive assay for detecting radiosensitive patients.
Acknowledgements. The author thanks Dr. M. Stumm, Magdeburg, and Dr. T. Dork, Hannover, for providing AT homozygote and heterozygote lyrnphoblastoid cell lines.
References
Barrios, L, Mir6, R, Caballin, MR, Fuster, C, Guedea, F, Subias, A, Egozcue, J (1989)
Cytogenetic effects of Radiotherapy. Breakpoint Distribution in induced chromosome aberrations. Cancer Genet Cytogenet 41, 61-70
Cigarran, S, Barrios, L, Barquinero, J, F, Caballin, MR, Ribas, M, Egozcue, J (1998)
Relationship between the DNA content of human chromosomes and their involvement in radiation-induced structural aberrations, analysed by painting. Int JRadiat
BioI 74, 449-455.
Gebhart, E, Neubauer, S, Schmitt, G, Birkenhake, S, Dunst J(1996) Use of a three-colour chromosome-in situ-suppression (CISS) technique for the cytogenetic detection
of past radiation exposure. Radiat Res 145, 47-52.
Greulich, KM, Kreja L, Heinze, B, Rhein, AP, Weier, HG, Bruckner, M, Fuchs P, Molls,
M (2000) Rapid detection of radiation-induced chromosomal aberrations in lymphocytes and hematopoietic progenitor cells by mFISH. Mutat Res 452, 73-81.
Lichter, P, Cremer, T, Borden, J, Manuelidis, L, Ward, DC (1988) Delineation of individual human chromosomes in metaphase and interphase cells by in situ suppression hybridization using recombinant DNA libraries. Hum Genet 80, 224-234.
Natarajan, AT, Vyas, RC, Darroudi, F, Vermeulen, S (1992) Frequencies of X-ray-induced chromosome translocations in human peripheral lymphocytes as detected by
in situ hybridization using chromosome-specific DNA libraries. Int JRadiat BioI 61,
199-203.
Neubauer, S, Gebhart, E, Schmitt, G, Birkenhake, S, Dunst J (1996) Is chromosome in
situ suppression (CISS) hybridization suited as a predictive test for intrinsic radiosensitivity in cancer patients? Int J Oncol 8, 707-712.