290
losE LUIS FERNANDEZ, VICENTE GOYANES, and IAIME GOSALVEZ
~.~ References
Fairbain OW, Olive PL, O'Neill KL (1995) The comet assay: a comprehensive review.
Mutation Res 339:37-59
Fernandez IL, Goyanes VI, Ramiro-Oiaz I, Gosalvez I (1998) Application ofFISH for in
situ detection and quantification of ONA breakage. Cytogenet Cell Genet 82:251-256
Rydberg B (1975) The rate of strand separation in alkali of irradiated mammalian cells.
Radiat Res 61:274-287
leukocyte after 30 s of unwinding and hybridisation with a whole genome probe. DAPI
counterstaining is shown in the upper right-hand corner. g Dual hybridisation with a whole
genome probe (red) and a telomeric probe (yellow-green), in unirradiated (top) and 20 Gy
irradiated (below) Chinese hamster Don cells. DNA breakage is simultaneously detected in the
whole genome and within the interstitial telomeric sequence areas, in the same cell. DAPI
counterstaining is presented on the left side. h Upper right-hand corner shows the signal
obtained in the alphoid DXZl locus after irradiation with 20 Gy of X-rays. The signal is
composed of dispersed spots, without continuity among them. The extended chromatin
fibre from a nucleus hybridised with a whole genome probe reveals that the size of these
spots is bigger than those making up the continuous fibre-FISH signal
losE LUIS FERNANDEZ, VICENTE GOYANES, and IAIME GOSALVEZ
~.~ References
Fairbain OW, Olive PL, O'Neill KL (1995) The comet assay: a comprehensive review.
Mutation Res 339:37-59
Fernandez IL, Goyanes VI, Ramiro-Oiaz I, Gosalvez I (1998) Application ofFISH for in
situ detection and quantification of ONA breakage. Cytogenet Cell Genet 82:251-256
Rydberg B (1975) The rate of strand separation in alkali of irradiated mammalian cells.
Radiat Res 61:274-287
leukocyte after 30 s of unwinding and hybridisation with a whole genome probe. DAPI
counterstaining is shown in the upper right-hand corner. g Dual hybridisation with a whole
genome probe (red) and a telomeric probe (yellow-green), in unirradiated (top) and 20 Gy
irradiated (below) Chinese hamster Don cells. DNA breakage is simultaneously detected in the
whole genome and within the interstitial telomeric sequence areas, in the same cell. DAPI
counterstaining is presented on the left side. h Upper right-hand corner shows the signal
obtained in the alphoid DXZl locus after irradiation with 20 Gy of X-rays. The signal is
composed of dispersed spots, without continuity among them. The extended chromatin
fibre from a nucleus hybridised with a whole genome probe reveals that the size of these
spots is bigger than those making up the continuous fibre-FISH signal