13 Combined in Situ Nick Translation and mRNA FISH for Simultaneous Detection
175
After blocking with bovine serum albumin (5% in 4xSSC), specific biotin
is detected with Cy3labeled streptavidin (1:150 in 4xSSC). After washing,
the tissue sections are counterstained with antifade solution containing
DAPI (1 Ilg/ml).
HE staining
Unprocessed photographs or digital images are taken from sections with
strong positive signals. In a following step, the same slide is stained routinely with hematoxylin and eosin. The same fields of vision are re-examined and photographed with a bright-field microscope for histomorphological analysis.
References
Bosari S, Moneghini L, Graziani D, Lee AK, Murray J}, Coggi G, Viale G (1995) Bcl-2
oncoprotein in colorectal hyperplastic polyps, adenomas, and adenocarcinomas.
Hum-Pathol 26(5): 534-540
Hell K, Lorenzen J, Fischer R, Hausmann ML (1995) Hodgkin cells accumulate mRNA
for bcl-2. Laboratory Investigation 73(4):492
Kikuchi Y, Dinjens WN, Bosman FT (1997) Proliferation and apoptosis in proliferative
lesions of the colon and rectum. Virchows-Arch. 431(2): 111-117
Mosnier JF, Perret AG, Vindimian M, Dumollard JM, Balique JG, Perpoint B, Boucheron S (1996) An immunohistochemical study of the simultaneous expression ofbcl-2
and p53 oncoproteins in epithelial tumors of the colon and rectum. Arch-PatholLab-Med. 120(7): 654-659
Mueller J, Mueller E, Hoepner I, Jutting J, Bethke B, Stolte M, Hofler H (1996) Expression of bcl-2 and p53 in de novo and ex-adenoma colon carcinoma: a comparative
immunohistochemical study. J-Pathol. 180(3): 259-265
Watson AJ, Merritt AJ, Jones LS, Askew IN, Anderson E, Becciolini A, Balzi M, Potten
CS, Hickman JA (1996) Evidence of reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. Br-J-Cancer. 73(8): 889-895
175
After blocking with bovine serum albumin (5% in 4xSSC), specific biotin
is detected with Cy3labeled streptavidin (1:150 in 4xSSC). After washing,
the tissue sections are counterstained with antifade solution containing
DAPI (1 Ilg/ml).
HE staining
Unprocessed photographs or digital images are taken from sections with
strong positive signals. In a following step, the same slide is stained routinely with hematoxylin and eosin. The same fields of vision are re-examined and photographed with a bright-field microscope for histomorphological analysis.
References
Bosari S, Moneghini L, Graziani D, Lee AK, Murray J}, Coggi G, Viale G (1995) Bcl-2
oncoprotein in colorectal hyperplastic polyps, adenomas, and adenocarcinomas.
Hum-Pathol 26(5): 534-540
Hell K, Lorenzen J, Fischer R, Hausmann ML (1995) Hodgkin cells accumulate mRNA
for bcl-2. Laboratory Investigation 73(4):492
Kikuchi Y, Dinjens WN, Bosman FT (1997) Proliferation and apoptosis in proliferative
lesions of the colon and rectum. Virchows-Arch. 431(2): 111-117
Mosnier JF, Perret AG, Vindimian M, Dumollard JM, Balique JG, Perpoint B, Boucheron S (1996) An immunohistochemical study of the simultaneous expression ofbcl-2
and p53 oncoproteins in epithelial tumors of the colon and rectum. Arch-PatholLab-Med. 120(7): 654-659
Mueller J, Mueller E, Hoepner I, Jutting J, Bethke B, Stolte M, Hofler H (1996) Expression of bcl-2 and p53 in de novo and ex-adenoma colon carcinoma: a comparative
immunohistochemical study. J-Pathol. 180(3): 259-265
Watson AJ, Merritt AJ, Jones LS, Askew IN, Anderson E, Becciolini A, Balzi M, Potten
CS, Hickman JA (1996) Evidence of reciprocity of bcl-2 and p53 expression in human colorectal adenomas and carcinomas. Br-J-Cancer. 73(8): 889-895