174
NORBERT HECKMANN
slide wilh Ii sue sC(;tion
•
deparaflinizalion
posl-fixing
proteinase K-digesl
slide wilh lissue scelion
o
prcdcnatur31ion
apoplosis dcIC(;lion
slide with lissue sC(;lion and
oligonucleotides
~
denaturation
hybridization
incubation
washing
dctcction
simultaneous demonstration of
apoplosis and
genes expression
DNA oligonucleolide
bioLinylalion using
Icrminallransferasc
biotinyluted oligonucleotidcs
•
prcdcnaturation
Fig. 1. Technique for simultaneous demonstration of apoptosis and gene expression