Antarctic Fish Immunoglobulins: Structure and Antibody Specificity
183
+20°C
+4'C
_2°C
1800
1200
350
1600
300
1400
1000
I 1200
f
250
~ 1000
~
800
200
~ 800
j
600
i
150
600
§
400
400
200
200
50
50
100
150
200
250
50
100
150
200
250
50
100
150
200
250
plasma dilution
plasma dilution
plasma dilution
Fig. 6. ELISA analysis: effects of temperature on the reaction of T. bernacchii plasma Ig
(-_-_-) with PdSP antigens. D. labrax plasma Ig (-e-e-) was used as negative control
The antigenic protein components of different nematode extracts were
compared, the PdSP 40 kDa antigen was identified as extracellular globin
and the PdSP 54 kDa antigen was purified. Finally, PdSP specific N
coriiceps antibodies were isolated and found heterogeneous in isoelectric
point.
Acknowledgments
This research was supported by a Grant from the Italian National Programme for
Antarctic Research. We thank Prof. G. di Prisco, Institute of Protein Biochemistry
and Enzymology, CNR, Naples, for providing us with nematodes and fish plasma
and secretions and Dr. S. Mattiucci, Institute of Parasitology, University "La
Sapienza," Rome, for the identification of nematode larvae.
References
1. Wilson MR, Warr GW (1992) Fish immunoglobulins and the genes that
encode them. Annual Rev ofFish Diseases 201-221
2. Warr GW (1995) The immunoglobulin genes of fish. Dev Comp Immunol
19: 1-12
3. Warr GW, Middleton DL, Miller NW, Clem LW, Wilson MR (1991) An
additional family of VH sequences in the channel catfish. Eur J Immunogen
18: 393-397
4. Bengten E, Stromberg S, Pilstrom L (1994) Immunoglobulin VH regions in
Atlantic cod (Gadus morhua L.): their diversity and relationship to VH
families from other species. Dev Comp Annual 18: 109-122
5. Roman T, Charlemagne J (1994) The immunoglobulin repertoire of the
rainbow trout (Oncorhynchus mykiss): defmition of nine IgH-V families.
Immunogenetics 40: 210-216
6. Coscia MR, Oreste U (1997) Immunoglobulins from Antarctic fish: structure
and antibody specificity. Proceedings of the third meeting on "Antarctic
Biology". Camerino University Press, pp 61-67
183
+20°C
+4'C
_2°C
1800
1200
350
1600
300
1400
1000
I 1200
f
250
~ 1000
~
800
200
~ 800
j
600
i
150
600
§
400
400
200
200
50
50
100
150
200
250
50
100
150
200
250
50
100
150
200
250
plasma dilution
plasma dilution
plasma dilution
Fig. 6. ELISA analysis: effects of temperature on the reaction of T. bernacchii plasma Ig
(-_-_-) with PdSP antigens. D. labrax plasma Ig (-e-e-) was used as negative control
The antigenic protein components of different nematode extracts were
compared, the PdSP 40 kDa antigen was identified as extracellular globin
and the PdSP 54 kDa antigen was purified. Finally, PdSP specific N
coriiceps antibodies were isolated and found heterogeneous in isoelectric
point.
Acknowledgments
This research was supported by a Grant from the Italian National Programme for
Antarctic Research. We thank Prof. G. di Prisco, Institute of Protein Biochemistry
and Enzymology, CNR, Naples, for providing us with nematodes and fish plasma
and secretions and Dr. S. Mattiucci, Institute of Parasitology, University "La
Sapienza," Rome, for the identification of nematode larvae.
References
1. Wilson MR, Warr GW (1992) Fish immunoglobulins and the genes that
encode them. Annual Rev ofFish Diseases 201-221
2. Warr GW (1995) The immunoglobulin genes of fish. Dev Comp Immunol
19: 1-12
3. Warr GW, Middleton DL, Miller NW, Clem LW, Wilson MR (1991) An
additional family of VH sequences in the channel catfish. Eur J Immunogen
18: 393-397
4. Bengten E, Stromberg S, Pilstrom L (1994) Immunoglobulin VH regions in
Atlantic cod (Gadus morhua L.): their diversity and relationship to VH
families from other species. Dev Comp Annual 18: 109-122
5. Roman T, Charlemagne J (1994) The immunoglobulin repertoire of the
rainbow trout (Oncorhynchus mykiss): defmition of nine IgH-V families.
Immunogenetics 40: 210-216
6. Coscia MR, Oreste U (1997) Immunoglobulins from Antarctic fish: structure
and antibody specificity. Proceedings of the third meeting on "Antarctic
Biology". Camerino University Press, pp 61-67