158
V. Carginale et al.
Fig. 7. Predicted secondary structures of the 5'-leader sequences of the MT mRNAs from
icefish (A) and T bernacchii (B). The arrow indicates the position of the start codon AUG
Possible secondary structures of the 5'-leader sequences from C.
hamatus and T. bernacchii mRNAs are depicted in Fig. 7. These
structures were determined by using the program MFOLD designed by
the University of Wisconsin Genetics Computer Group. As shown in Fig.
7, the 5'-leader sequence of the C. hamatus mRNA (A) displays an
additional stem loop structure which is absent in the 5' UTR of the T
bernacchii mRNA (B).
The expression of MT transcripts was assessed by Northern blotting of
total liver RNA using C. rastrospinosus MT cDNA as probe. The results
depicted in Fig. 8 show that the amounts of mRNA in icefish are
markedly higher than those in the red-blooded fish.
V. Carginale et al.
Fig. 7. Predicted secondary structures of the 5'-leader sequences of the MT mRNAs from
icefish (A) and T bernacchii (B). The arrow indicates the position of the start codon AUG
Possible secondary structures of the 5'-leader sequences from C.
hamatus and T. bernacchii mRNAs are depicted in Fig. 7. These
structures were determined by using the program MFOLD designed by
the University of Wisconsin Genetics Computer Group. As shown in Fig.
7, the 5'-leader sequence of the C. hamatus mRNA (A) displays an
additional stem loop structure which is absent in the 5' UTR of the T
bernacchii mRNA (B).
The expression of MT transcripts was assessed by Northern blotting of
total liver RNA using C. rastrospinosus MT cDNA as probe. The results
depicted in Fig. 8 show that the amounts of mRNA in icefish are
markedly higher than those in the red-blooded fish.